六点挤压式核桃破壳机的正交试验研究

为了优化自主设计的六点挤压式核桃破壳机的最佳参数,选择含水率,挤压行程,破壳速度三个因素做正交实验,最终由试验结果可得,六点挤压式核桃破壳机的破壳最优条件是核桃的挤压行程9mm,含水率20%,破壳速率1s/个,且经过试验验证得出最佳破壳综合指标在0.972左右。     

Adiponectin‐impaired adipocyte differentiation negatively regulates fat deposition in chicken

Adiponectin is a protein hormone secreted exclusively by adipocytes that plays an important role in the modulation of glucose and lipid metabolism. To investigate the effect of adiponectin on lipid metabolism in chicken, rosiglitazone (agonist of adiponectin) and dexamethasone (inhibitor of adiponectin) were used to treat 23‐day‐old broilers in vivo. To verify the functionality of adiponectin on fat deposition, chicken pre‐adipocytes were cultured in the medium containing 10 μg/ml adiponectin. Serum adiponectin and lipids and fat distribution were analysed. Oil Red O staining was used to determine lipid deposition in adipocytes. The expression levels of adiponectin, adiponectin receptors (AdipoR) and lipid metabolism–related genes in different tissues and pre‐adipocytes were measured using real‐time PCR, and the abundance of lipid metabolism–related proteins was measured by Western blot. Rosiglitazone increased serum adiponectin concentration and the expression levels of adiponectin and adiponectin receptor 1 (AdipoR1) in tissues and significantly decreased levels of serum lipids and fat deposition. Rosiglitazone significantly increased the expression levels of adipose triglyceride lipase (ATGL) and AdipoR1 and decreased the expression levels of fatty acid synthase (FAS). Dexamethasone had the converse effects compared with rosiglitazone. Oil red O staining results showed a marked decrease in fat deposition in cells treated with adiponectin. In adipocytes, adiponectin could decrease the expression levels of CCAAT/enhancer‐binding protein α (C/EBPα) and FAS and increased the expression levels of ATGL and AdipoR1. These results indicate that adiponectin has a remarkable effect on impairment of adipocyte differentiation, which contributes to the negative regulation of fat deposition in chicken.     

Leucine markedly regulates pancreatic exocrine secretion in goats

Four goats (30.1 ± 1.3 kg) with common bile duct re‐entrant catheter and duodenal catheter were used to evaluate the effects of duodenal leucine infusion on pancreatic exocrine secretion and plasma parameters with two 4 × 4 Latin square design experiments. In the long‐term infusion experiment, goats were fed twice daily [700 g/day, dry matter (DM) basis] at 8:00 and 18:00 hours and were duodenally infused with 0, 3, 6, 9 g/day leucine for 14 days. Pancreatic juice and jugular blood samples were collected over 1‐h intervals for 6 h daily from d 11 to 14 days to encompass a 24‐h day. In the short‐term experiment, goats were infused leucine for 10 h continuously at the same infusion rate with Experiment 1 after feed deprivation for 24 h repeated every 10 days. Pancreatic juice and blood samples were collected at 0, 1, 2, 4, 6, 8 and 10 h of infusion. The results showed that the long‐term leucine infusion did not affect pancreatic juice secretion, protein output, trypsin and lipase secretion and plasma insulin concentration, but linearly increased α‐amylase secretion. No changes in pancreatic protein and lipase secretion were observed in the short‐term infusion. Pancreatic juice and α‐amylase secretion responded quadratically, with the greatest values observed in the 3 and 6 g/day leucine respectively. Trypsin secretion linearly decreased, while plasma insulin concentration increased linearly with increased leucine infusion. The results demonstrated that duodenal leucine infusion dose and time dependently regulated pancreatic enzyme secretion not associated with the change in plasma insulin concentration.     

人类活动对河岸带植被氮磷生态化学计量特征的影响——以汾河临汾段为例

[目的]探究自然河岸带和强人工干扰河岸带土壤—植被系统氮、磷生态化学计量的变化规律,为该区域的河岸带修复和环境保护提供科学依据.[方法]利用相关分析和偏冗余分析等方法,以汾河临汾段典型河岸带为例,探讨自然河岸带及强人工干扰河岸带土壤—植被系统氮、磷含量的时空分布特性及其影响因素.[结果]河岸带植被总氮(TN),总磷(T...     

牛精液冷冻保护剂研究进展

牛的人工授精技术是目前应用最为广泛的动物繁殖技术之一,对牛的遗传改良做出了巨大贡献。但是,在冷冻一解冻过程中仍然有大约40％～50％的精子失去活性,限制良种公牛种用性能的发挥。论文在分析精子冷冻保存原理的基础上,阐述了渗透性、非渗透性冷冻保护剂以及低密度脂蛋白对牛精子的冷冻保护作用,以期为开发新型冷冻保护剂的研究提供一定参考,进一步提升牛冷冻精液的质量。     

陕西省生猪规模养殖成本调查分析

通过对陕西省生猪规模养殖场户生产数据的分析,研究生猪规模养殖成本的构成,对控制生猪养殖成本提供依据,从成本投入的角度分析陕西适宜的生猪养殖规模,揭示规模化和专业化是生猪养殖的发展方向,提出推进规模化养殖的建议.     

Establishment and characterization of a dairy goat mammary epithelial cell line with human telomerase (hT‐MECs)

Although research on dairy goat mammary gland have referred extensively to molecular mechanisms, research on lines of dairy goat mammary epithelial cells (MECs) are still rare. This paper sought to establish an immortal MEC line by stable transfection of human telomerase. MECs from a lactating (45 days post‐parturition) Xinong Saanen dairy goat were cultured purely and subsequently transfected with a plasmid carrying the sequence of human telomerase. Immortalized MECs by human telomerase (hT‐MECs) exhibited a typical cobblestone morphology and activity and expression levels of telomerase resembled that of MCF‐7 cells. hT‐MECs on passage 42 grew vigorously and ‘S’ sigmoid curves of growth were observed. Moreover, hT‐MECs maintained a normal chromosome modal number of 2n = 60, keratin 8 and epithelial membrane antigen (EMA) were evidently expressed, and beta‐casein protein was synthesized and secreted. Beta‐casein expression was enhanced by prolactin (P < 0.05). Lipid droplets were found in hT‐MECs, and messenger RNA levels of PPARG, SREBP, FASN, ACC and SCD in hT‐MECs (passage 40) were similar to MECs (passage 7). In conclusion, the obtained hT‐MEC line retained a normal morphology, growth characteristics, cytogenetics and secretory characteristics as primary MECs. Hence, it can be a representative model cell line, for molecular and functional analysis, of dairy goat MECs for an extended period of time.     

羧甲基纤维素钠对壤砂土水分运动及水力参数的影响

科学使用羧甲基纤维素钠(CMC)实现保水控盐对于滨海壤砂盐碱土改良具有重要意义,而明晰CMC对滨海壤砂土水分运动规律的影响是科学使用CMC的重要基础。为研究施加CMC滨海壤砂土水分运动规律,本文通过开展一维垂直土柱积水入渗试验,探索不同CMC施量(0、0.1、0.2、0.4、0.6 g?kg-1) 对壤砂土入渗特性、水分分布和土壤水力参数的影响。结果表明,施用CMC土壤的最终累积入渗量增加了4.90％～15.17％、达到预设湿润锋深度的入渗时间增加了61.90%～604.73%;Philip入渗模型参数吸渗率S和Green-Ampt模型参数KsSf均随CMC施量的增加而减少,吸渗率S和平均土壤水扩散率与CMC施量之间的数学关系分别可用二次多项式和指数函数来表示;CMC增强了土壤的持水能力,土壤剖面含水量提高了0.72%～3.74%;CMC通过改变土壤结构影响了土壤水力参数,滞留含水率θr、饱和含水率θs及进气吸力倒数α均与CMC施量呈正相关关系,而与饱和导水率Ks和形状系数n呈反比关系。通过对变异系数CV的分析发现,CMC对饱和导水率Ks和进气吸力倒数α影响表现为中等差异,对滞留含水率θr、饱和含水率θs和形状系数n表现为弱差异。研究结果揭示了CMC对滨海壤砂土减渗保水的内在机理,为滨海盐碱地的改良提供了理论参考。     

基于成本视角的水质水量双向调节生态补偿量研究

[目的]以黄河流域陕西片区为实例进行水质水量双向调节的生态补偿量研究,为解决黄河流域上下游生态权益分配矛盾,实现流域区域间公平发展提供理论依据。[方法]同时考虑成本、价值和奖惩3个方面,以基本补偿标准为基础,采用水质改良系数法和水量贡献度法,构建基于水质水量的补偿标准测算模型开展研究。[结果]黄河流域陕西片区生态补偿量的基本补偿标准为13.74亿元;基于水质、水量调节值分别为-5.66和6.84亿元;2018年陕西省获得生态补偿金额为14.92亿元。[结论]相比以往的测算模型,该模型的测算结果更符合实际情况,同时也更适应于外部环境要素的变化。     

植被恢复和覆土厚度对砒砂岩区土壤水分及养分的影响

[目的]研究植被恢复对砒砂岩区土壤水分和养分的影响状况,明确对于砒砂岩地区土壤保水保肥效果最佳的植被恢复类型,为砒砂岩区的生态修复和区域水土流失治理提供理论依据。[方法]选取准格尔旗暖水乡裸露砒砂岩向覆土砒砂岩过渡区域,以黄土—砒砂岩交界带不同人工恢复植被和自然恢复草地的土壤为研究对象,测定土壤水分和养分含量,分析不同植被恢复和覆土厚度对砒砂岩区土壤水分和养分特征的影响(其中天然草地作为对照)。[结果](1)裸露砒砂岩向覆土砒砂岩过渡区域土壤水分含量主要受黄土厚度影响,黄土的持水性能优于砒砂岩土壤,养分含量主要受植被恢复类型影响;(2)研究区土壤各指标均属于中等变异程度,其中土壤水分、碳、氮含量自北向南随覆土厚度增加逐渐增加,磷素分布则相反;(3)对比天然草地,沙棘和油松恢复对砒砂岩区土壤碳、氮含量的提升效果最好,土壤有机质含量分别提高了43.12%和34.27%,全氮提高了78.95%和42.11%,铵态氮提高25.64%和46.15%,硝态氮提高69.44%和42.22%。其中油松恢复下土壤水分含量高于天然草地54.55%,但人工植被恢复后的土壤磷素水平并没有提升。[结论]黄土较砒...