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We describe the spatial epidemiology of Varroa destructor infestation among honey bee apiaries in the greater Auckland area of the North Island of New Zealand. The study population was comprised of 641 apiaries located within the boundaries of the study area on 11 April 2000. Cases were those members of the study population declared Varroa-infested on the basis of testing conducted between April and June 2000. The odds of Varroa was highest in apiaries in the area surrounding transport and storage facilities in the vicinity of Auckland International Airport. A mixed-effects geostatistical model, accounting for spatial extra-binomial variation in Varroa prevalence, showed a 17% reduction in the odds of an apiary being Varroa infested for each kilometre increase in the squared distance from the likely site of incursion (95% Bayesian credible interval 7–28%). The pattern of spatially autocorrelated risk that remained after controlling for the effect of distance from the likely incursion site identified areas thought to be ‘secondary’ foci of Varroa infestation initiated by beekeeper-assisted movement of infested bees. Targeted investigations within these identified areas indicated that the maximum rate of local spread of Varroa was in the order of 12 km/year (interquartile range 10–15 km/year). 相似文献
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桃果实有毛/无毛性状的SCAR标记 总被引:11,自引:4,他引:11
以桃品种‘京玉’和‘美味’的正反交69株F1 群体为试材, 利用RAPD技术扩增出了与桃果实有毛/无毛性状(G/ g) 连锁的2 258 bp的多态性片段, 经克隆、测序后, 根据获得的序列重新设计了两对引物进行SCAR转化。引物对BFP94 /BFP95在有毛和无毛个体中均扩增出了2 258 bp的片段, RAPD显示的多态性消失。利用引物对BFP96 /BFP98成功将RAPD标记转化成了SCAR标记, 并命名为SCP2022258。该标记仅在果实有毛的个体中出现, 与有毛/无毛性状的连锁距离为718 cM, 且扩增稳定, 为桃果实有毛/无毛性状育种的分子标记辅助选择奠定了基础。 相似文献
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坪用野牛草种质资源搜集及品种选育研究进展 总被引:3,自引:0,他引:3
野牛草是一种抗逆性强、管理粗放的草坪植物,非常适合我国一些缺水城市水土保持和园林绿化的需要,但也存在绿期较短、颜色较浅、雄株坪用景观不好等缺点.近年来,通过对野牛草种质资源进行鉴定和评价,从中选择了大量优良的种质材料,使其坪用缺点逐步改善,并利用有性杂交和生物技术等育种手段培育出了许多优良坪用品种. 相似文献
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Verbeylen Goedele De Bruyn Luc Adriaensen Frank Matthysen Erik 《Landscape Ecology》2003,18(8):791-805
In determining isolation effects in fragmented populations, the landscape matrix is not often considered. Usually simple distance
measures are used to quantify degree of isolation. We tested the effect of the matrix on the presence of red squirrels in
354 wooded patches in the Brussels Region, by comparing several isolation measures. These were 1) distance to the nearest
source patch, 2) the Hanski-measure (a combination of distance to and size of all possible sources), 3) effective distances
calculated from different least cost models using the ArcView grid extension ‘Cost Distance’ (a combination of distance and
resistance of the landscape, with different resistances for different landscape types) and 4) some combinations of the Hanski-measure
and the effective distances. Size and quality of the target patches were always included in the tests of the predictive power
of different isolation measures on squirrel presence/absence. All variables examined (patch size, quality and isolation) significantly
influenced squirrel presence. Models using the effective distances gave the best results. Models including the Hanski-measure
improved significantly when Euclidean distance was replaced by effective distance, showing that parameterisation of matrix
resistance added significant additional explanatory power when modelling squirrel presence.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
60.
Wanyoike Mary Wanjiru Kang Zhensheng Heinrich Buchenauer 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(8):803-810
Cytological studies were carried out to elucidate the importance of cell wall degrading enzymes (CWDE) during infection of wheat spikes by Fusarium graminearum. Scanning electron micrographs revealed that at 6–24 hours after inoculation (hai) of single spikelets with macroconidia of F. graminearum, the fungus germinated by forming several germ tubes and developed a dense hyphal network in the cavity of the spikelet. At 24–36hai, the fungus formed infection hyphae which invaded the ovary and inner surface of the lemma and palea. Transmission electron microscopical studies revealed that the fungus extended inter- and intracellularly in the ovary, lemma and rachis and caused considerable damage and alterations to the host cell walls. In different tissues of healthy and F. graminearum-infected wheat spikes the cell wall components cellulose, xylan and pectin were localized by means of enzyme-gold and immuno-gold labelling techniques. Localization of cellulose, xylan and pectin showed that host cell walls which were in direct contact with the pathogen surface had reduced gold labelling compared to considerable higher labelling densities of walls distant from the pathogen–host interface or in non-colonized tissues. The reduced gold labelling densities in the infected host cell walls indicate that these polysaccharide degrading enzymes might be important pathogenicity factors of F. graminearum during infection of wheat spikes. The results revealed that, infection and colonization of wheat spikes by F. graminearum and reactions of infected host tissue were similar to those reported for F. culmorum. 相似文献