关于地理空间数据整合与持续高效更新存在的问题分析

文章针对当前地理空间数据整合与持续高效更新所存在的问题,在地理空间数据更新中引入了“数据同化”这一思想和概念,对持续高效更新存在的问题及“地理空间数据同化”的涵义和关键技术进行了论述.     

Atmospheric humidity and genotype are key determinants of the diurnal stomatal conductance pattern

Great understanding of the genotypic difference in diurnal stomatal conductance (g_s) pattern and the key determinants of the pattern is important for saving water by adopting cultivars appropriately. Fifteen wheat genotypes were studied under different soil conditions and various meteorological conditions with pot cultivation in rain shelter for two years. Genotype and air humidity were found to be key determinants of diurnal g_s pattern. All genotypes under low relative humidity (LRH) and most genotypes under high relative humidity (HRH) displayed a gradual decline pattern from morning through the afternoon. Under moderate relative humidity (MRH), all genotypes present a single-peak curve pattern, but they differed in peak time, which may lead to unreliable g_s comparison between genotypes and get ostensible contrasting materials. The stomatal conductance was significantly different among genotypes under LRH and the increased g_s magnitude is also significantly different when it was compared between LRH and HRH. The present results provide new thinking for selecting and adopting appropriate cultivars with specific stomata traits for the area with various meteorological conditions.     

Land surface temperature retrieval for arid regions based on Landsat-8 TIRS data:a case study in Shihezi,Northwest China

Scientific interest in geophysical information about land surface temperature （LST） is ever increasing, as such information provides a base for a large number of applications, including environmental and agricultural monitoring. Therefore, the research of LST retrieval has become a hot topic. Recent availability of Landsat-8 satel- lite imagery provides a new data source for LST retrieval. Hence, exploring an adaptive method with reliable ac- curacy seems to be essential. In this study, basing on features of Landsat-8 TIRS thermal infrared channels, we re-calculated parameters in the atmospheric transmittance empirical models of the existing split-window algorithm, and estimated the ground emissivity with the help of the land cover classification map of the study area. Further- more, a split-window algorithm was rebuilt by virtual of the estimation model of the updated atmospheric transmit- tance and the ground emissivity, and then a remote sensing retrieval for the LST of Shihezi city in Xinjiang Uygur autonomous region of Northwest China was conducted on the basis of this modified algorithm. Finally, precision validation of the new model was implemented by using the MODIS LST products. The results showed that the LST retrieval from Landsat-8 TIRS data based on our algorithm has a higher credibility, and the retrieved LST is more consistent with the MODIS LST products. This indicated that the modified algorithm is suitable for retrieving LST with competitive accuracy. With higher resolutions, Landsat-8 TIRS data may provide more accurate observation for LST retrieval.     

Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells

AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G₀/G₁ phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells.     

基于SHAW模型的冬小麦近地面层气温模拟

利用一维多层水热耦合SHAW（The simultaneous heat and water）模型,在田间实验的基础上,模拟河南省商丘地区2015年冬小麦拔节后近地面层0~40 cm垂直方向上的每小时气温变化特征。结果表明,冬小麦近地面层气温模拟整体效果较好,其中48%模拟的绝对误差低于1℃,75%模拟的绝对误差低于2℃,不同高度上模型效率ME均大于0.94;夜晚气温的模拟效果优于白天的模拟效果,白天11:00—14:00气温被过低估计,并随着近地面层高度的增加,模拟值误差越大;近地面层内3种气温特征值模拟效果的优劣依次为：日平均气温、日最低气温、日最高气温,其中,日平均气温模拟值与实测值基本吻合,日最低气温被略微高估,日最高气温被过低估计。此外,SHAW模型在冬小麦拔节后6个生育期的模拟效果均存在差异,拔节期、灌浆期和乳熟期模拟效果较好,孕穗期和开花期次之,抽穗期模拟效果相对较差。     

豫东地区能源型甘薯品种的筛选

为选育适合豫东地区自然条件的高产高淀粉型甘薯品种,在河南省商丘市进行了生产试验,并测定各品种的鲜薯产量、薯干产量、干物质量、淀粉率及主要病虫害抗性。结果表明:徐薯22、商薯19和金玉3种能源生产专用型甘薯品种能更好地适应当地条件,其淀粉含量比当地主栽品种豫薯9号提高10%左右,产量提高5%～10%。大力发展能源型甘薯种植能使农民增收,使用生物质能源可有效减轻大气污染,经济效益、社会效益和生态效益明显。     

砂壤土强筋小麦应用杀菌剂对产量和品质的影响

运用田间小区试验方法,在豫东平原砂壤土生态类型区麦田,初步探讨了杀菌剂对强筋小麦产量及品质的影响。试验结果表明,使用三唑酮、多菌灵、灭菌丹、烯唑醇及病虫灵等5种杀菌剂,强筋小麦千粒重提高0.8～1.9g,产量增加147.6～471.3kg/hm2,平均增产5.4%。于小麦抽穗后喷洒1次杀菌剂效果较好,增加喷洒次数以后对强筋小麦品质有不良影响。不同生育期应用杀菌剂,基本上不影响强筋小麦的品质,其应用效果主要取决于杀菌剂种类及喷洒次数。既使强筋小麦增产,又基本不影响其品质指标的杀菌剂为三唑酮和烯唑醇,其中又以三唑酮的增产效应最为突出,一般增产8%以上,千粒重提高2g左右,生产中可以考虑优先选用。     

猪丁型冠状病毒在悬浮培养猪肾细胞LLC-PK1上的增殖特性分析

旨在分析猪丁型冠状病毒(porcine deltacoronavirus,PDCoV)在悬浮培养的猪肾细胞LLC-PK1上的增殖特性,为PDCoV灭活疫苗的规模化生产提供细胞材料。采用逐步降血清法优化LLC-PK1细胞悬浮培养工艺;利用有限稀释法筛选PDCoV适应性细胞株;利用间接免疫荧光法鉴定PDCoV对LLC-PK1细胞的感染性;分别对PDCoV接种LLC-PK1悬浮细胞的初始密度、MOI、收毒时间、TPCK胰酶浓度等参数进行优化,确定最佳悬浮培养条件。成功筛选出可高效增殖PDCoV的单克隆悬浮细胞株LLC-PK1Sa,且利用其增殖的PDCoV可特异性的感染LLC-PK1细胞;PDCoV按MOI为10^-3接种于密度为2×10⁶ cells·mL^-1的LLC-PK1Sa细胞,当TPCK胰酶终浓度达到7.5 μg·mL^-1时,接毒后48 h收获的病毒液滴度最高。本研究首次实现了PDCoV在LLC-PK1Sa悬浮细胞中的高效增殖,并对悬浮培养条件进行了初步优化,可为PDCoV灭活疫苗的规模化生产提供理论参考。     

Isolation,Identification, 16S rRNA Gene Sequencing and Genetic Evolution Analysis of Aeromonas aeruginosa from Duck

【Objective】 The experiment was aimed to study the pathogenic bacteria of acute death of ducklings in a duck farm in Henan province and its phylogenetic status.【Method】 The liver and spleen of dead ducks in the diseased duck farm were collected.The bacterial morphology, Gram-staining, biochemical characteristics, sequence analysis of 16S rRNA gene, drug sensitivity test and pathogenicity test were carried out.【Results】 The isolated bacteria grew on blood agar medium with smooth, convex, milky white and α-hemolytic Gram-positive cocci.Biochemical test results showed that the isolated bacteria were positive for maltose, sucrose, raffinose, nitrate reduction reaction, MP-VP test, urea, lysine decarboxylase, ornithine decarboxylase and aescin, and negative for xylose, lactose, glucose, sorbitol, hydrogen sulfide, citrate, peptone, mannitol, phenylalanine and methyl red.The results of 16S rRNA gene sequencing and BLAST comparison results showed that the 16S rRNA gene sequence of the isolated strain was 94.8%-99.9% similar to that of Aerococcus viridans published on NCBI, and the similarity with strains Mnlv1, Mnlv2 and W66 was the highest, up to 99.9%.The similarity with strain GXBl-1 was the lowest, which was 94.8%.Phylogenetic tree analysis showed that the isolated bacteria belonged to the same genus and group on the same branch as strains of Aerococcus viridans FL09, 15MS and Mnlv2 etc., and had the closest genetic relationship with FL09.The results of drug sensitivity showed that the isolated strain was sensitive to fosfomycin, ampicillin and amoxicillin, moderately sensitive to penicillin, neomycin and amikacin, it was resistant to rifampicin, neomycin, bacitracin, erythromycin and enrofloxacin.The results of pathogenicity test showed that the strain was lethal and increased with the increase of inoculation concentration.【Conclusion】 This study determined that the pathogen causing the acute death of ducklings in a duck farm in Henan province was Aerococcus viridans, which provided a reference basis for clinical diagnosis and treatment of the diseases caused by this bacteria.     

复合农林业系统碳贮量研究进展

CO2作为一种主要温室气体,其固存与排放对全球气候变化有重要影响。本文以复合农林业系统为研究对象,介绍了农林复合类型中林木、农作物、凋落物、土壤碳贮量的估算方法,并指出目前碳贮量研究方面的问题和发展方向,为碳循环研究提供部分基础研究数据和理论支持。