肾上腺皮质肿瘤良恶性鉴别方法探究

肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）是一种常见的肿瘤,存在于人体的内分泌系统中,并且是引发肾上腺皮质癌（Adrcnocortical Carcinoma,ACC）进而导致死亡的主要原因.经典的＂Weiss系统＂在诊断明确性上存在种种无法解决的问题,但随着科技水平的发展,人们开始探索新的途径来鉴别ACT的良恶性,于是开始有了影像学和分子生物学技术等特异性的检查方法,从而进一步增强了诊断的明确性.现通过对比的方式,探究肾上腺皮质肿瘤（Adrenocortical Tumor,ACT）影像学检查和分子生物学技术检查的结果,从而能更准确地鉴别其良恶性.     

Nanostructured liquid crystalline particle assisted delivery of 2,4-dichlorophenoxyacetic acid to weeds,crops and model plants

Routine agricultural practices are heavily dependent on the use of surfactants, many of which are toxic to humans and detrimental to the environment. In proof of concept work we have previously shown the potential of nanostructured liquid crystalline particles (NLCP) to safely interact with plant leaf cuticular surfaces with minimal impact on epicuticular waxes. Here we demonstrate the use of NLCP to effectively deliver the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) to plant leaves in laboratory and field studies. In the laboratory, the physiological stress responses of lupin, Lupinus angustifolius (L.) (Fabaceae) towards NLCP spray applications were shown to be much reduced in comparison with application of two common surfactants. Phytotoxicity assays of 2,4-D loaded NLCP were used to validate the herbicidal effects on Arabidopsis thaliana (L.) Heynth. (Brassicaceae) and established a similarity with that of surfactant assisted 2,4-D delivery when tested at a concentration of 0.1%. Field trials were conducted to test the efficacy of NLCP-assisted delivery of 2,4-D in comparison with commercial surfactants for the control of the invasive weed wild radish, Raphanus raphanistrum (L.) (Brassicaceae), in wheat, Triticum aestivum (L.) (Poaceae) crop fields. Compared against Estercide 800, a commercially available 2,4-D formulation, NLCP assisted delivery of 2,4-D was effective at low concentrations of 0.03% and 0.06%. The crop yield remained similar for all the tested concentrations and formulations of 2,4-D loaded NLCP and Estercide 800. This is the first report to directly show that, as an alternative to conventional methods, NLCP can be used under both laboratory and field conditions to successfully delivery an agrochemical.     

犬冠状病毒基因疫苗表达载体的构建及其免疫原性研究

以pVAX1为载体首次构建了犬冠状病毒病基因的3种真核表达质粒。首先将犬冠状病毒大熊猫株（CCVDXMV）纤突蛋白（S）、膜蛋白（M）和核蛋白（N）基因进行了克隆和序列测定。将测序后的质粒pTS、pTM和pTN分别双酶切，回收目的基因片段并将其定向克隆到真核表达质粒pVAX1中得到重组质粒pVAXS、pVAXM和pVAXN。将这3种真核表达质粒通过脂质体介导法转染MDCK细胞，通过RT—PCR法进行转录水平的检测，并用间接ELISA法检测目的蛋白的表达情况。结果在pVAXS、pVAXM、pVAXN转染MDCK细胞36h后就可检测到目的基因的转录；在转染72h后可检测到3种目的蛋白的表达。动物免疫试验表明，3种真核表达载体能有效地诱导机体产生细胞免疫与体液免疫应答，这为CCV基因疫苗的研究奠定了良好的基础。     

基于RS和GIS的吉林省城乡居民地区划特征分析

探讨吉林省的城乡居民地的空间格局对于城镇建设和相关基础设施建设无疑具有很大的参考价值。本文以TM为数据源提取了吉林省居民地空间分布信息。在GIS技术支撑下,结合区划理论和聚落地理学说,分析了吉林省城乡居民地的空间格局。结果表明,吉林省城乡居民地空间分布区划为三个区,分别是东部区、中部区和西部区。中部区城镇居民地和农村居民地密集,且城镇化程度最高;东、西部区城镇居民地景观指数无明显差别,但农村居民地的景观指数明显有差别。从居民地分布类型来看,城镇居民地与农村居民地在各区内都呈随机分布,但其指示参数最近邻点指数还是体现出明显的区划差异。     

《艺文类聚》辑录文献方法述略

《艺文类聚》辑录文献的基本原则是“以类相从”,这是对前代图书编辑成果的继承。具体方法有“子目标题法”和“篇题法”2种。     

松嫩平原碱水水域对虾体重生长速度与Ca~(2+)、Mg~(2+)的关系

以盐碱化湖泊和池塘水进行凡纳滨对虾(Litopenaeus vannamei Boone,1931)幼虾生长试验,探讨碱水环境对虾体重生长速度与Ca2 、Mg2 、Ca2 Mg2 质量浓度及(1/2Ca2 )/(1/2 Mg2 )的关系,为碱水水域养殖提供科学依据.结果表明,在碱度为10.32～38.50 mmol/L、盐度1.02～3.34g/L、pH 8.5～9.5的水环境中,体长为10～20 mm的幼虾饲养27 d的体重生长速度为(0.95 0.18)mg/d,存活率(47.5±14.5)%.不同水环境存活率差异显著(P＜0.01),体重生长速度差别不明显(P＞0.05).体重生长速度与环境因子问的相关性不明显.通过驯化提高幼虾对水环境的综合适应能力,是碱水水域养殖对虾的重要途径.     

Isocyanate curing of novolac-type ligno-phenol–formaldehyde resins

This work proposes the use of a diisocyanate as curing agent for novolac-type lignin–phenol–formaldehyde (LPF) resins, instead of typical hexamethylene–tetraamine (HMTA), with the aim of overcoming the loss of reactivity of lignophenolic resins resulting from the introduction of lignin. Curing LPF resins with methylene–diisocyanate (MDI) and HMTA has been investigated through Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC) and rheological analysis. The analysis of experimental results shows that the proposed curing reaction with MDI gives similar results to those for HMTA-cured systems. However, MDI curing is faster than HMTA one and proceeds easier at lower temperatures.