Effects of Wnt/β-catenin signal pathway on asthma airway remodeling

AIM: To explore the effect of Wnt/β-catenin signaling pathway in airway smooth muscle cells (ASMC) on asthmatic airway remodeling.METHODS: The asthmatic airway remodeling model in rats was established and the ASMC was isolated and cultured. The protein expression of β-catenin, glycogen synthase kinase-3β (GSK-3β), c-Myc and cyclin D1 in the ASMC was determined by Western blot. After depressing the interaction between β-catenin and p300/CBP, the cell activity was measured by CCK-8 assay and the change of cell cycle distribution was analyzed by flow cytometry. Meanwhile, the protein expression of c-Myc and cyclin D1 in the ASMC was determined by Western blot after inhibiting P38 mitogen-activated protein kinase (MAPK) activity.RESULTS: The protein levels of β-catenin, c-Myc and cyclin D1 were significantly increased in asthma group while the protein level of GSK-3β was decreased in the same group (P<0.05). After depressing the interaction between β-catenin and p300/CBP, the cell activity of ASMC was decreased in asthma group compared with control group (P<0.05), and the change of the cell cycle distribution in asthma group was also more obvious (P<0.05). After inhibiting P38 MAPK activity, the protein levels of c-Myc and cyclin D1 were all decreased compared with control group in ASMC asthma and control rats (P<0.05).CONCLUSION: Wnt/β-catenin signaling pathway may participates in airway remodeling in asthma by increasing the protein expression of c-Myc and cyclin D1, reacting with the P38 MAPK signaling pathway and regulating the growth of ASMC.     

稻鱼系统中不同沟型边际弥补效果及经济效益分析

稻田养鱼在中国由来已久,并已经发展出各种模式。在沟坑模式中,作为鱼群庇护所的沟和坑会占用一定面积的水田区域,致使水稻立植面积减少,但沟坑的设置同时导致水稻群体边际区域的增加。本试验就沟坑边际效应导致的水稻增产效应对因立植面积减少而引起的产量减少的弥补效果进行了试验研究和测定计算,同时对环沟、十字沟、直沟3种沟型下水稻产量、田鱼产量及经济收益进行了比较分析,筛选出适合试验区域的稻鱼共生模式中的最佳沟坑模式。随机区组田间试验结果表明,沟际边1行单蔸粒重的边际效应值平均可达52.45%;沟坑边际效应弥补效果也较为显著,平均达80%左右。且不同沟型弥补效果不一:环沟弥补效果最佳,达95.89%,几乎可弥补沟坑占地损失;十字沟次之,为85.58%;直沟最差,仅可弥补58.02%。各类沟型的水稻稻谷产量及田鱼产量差异均不显著,而产出收益以十字沟表现最佳。研究认为十字沟为稻田养鱼模式下的最优沟型。     

江西省近46年蒸发皿蒸发量变化特征及其影响因素分析

利用江西省79个常规气象站1960-2005年的观测资料,综合分析了蒸发皿年蒸发量的变化特征及其与相关环境因子包括日照百分率、云量、气温(日平均温度、日较差、最高温度、最低温度)、10m风速、饱和水汽压差、年降水量和相对湿度的相关关系,试图找出蒸发皿年蒸发量变化的主要原因。结果表明:(1)江西省西部及中部年蒸发量较小;南部及鄱阳湖周边地区年蒸发量较大。在过去46a中,蒸发皿年蒸发量呈显著下降趋势,其速率为-4.57mm/a;79站中,有68站蒸发皿年蒸发量下降趋势显著;(2)以日照百分率为代表的能量供给因子与蒸发皿年蒸发量的相关系数最大,其次为动力因子(10m风速),以相对湿度为代表的湿度因子相对较小,三者的完全相关系数分别为0.37、0.33、0.23;(3)空间上,整个江西省受日照百分率影响均比较大,风速、相对湿度对江西省的影响在鄱阳湖附近、西南部分地区更大,日照百分率、风速的降低以及相对湿度的增加导致了蒸发皿蒸发量的减小;(4)利用日照百分率、风速、相对湿度拟合的蒸发皿年蒸发量多元回归方程,能够反映该地区平均年蒸发量的情况,精度较高。     

Adenosine promotes bFGF protein and bFGF mRNA expression in human umbilical vein endothelial cells in vitro

AIM: To investigate the influence of adenosine on human umbilical vein endothelial cells (HUVEC) bFGF protein production and bFGF mRNA expression. METHODS: Immunohistochemistry staining was performed to detect bFGF protein. RT-PCR was performed to detect bFGF mRNA expression. RESULTS: Immunohistochemistry study demonstrated that there was only a small amount of bFGF positive cells and the color was weak in control group (without adenosine). In groups treated with 10-4 mol/L and 10-6 mol/L adenosine, bFGF protein was significantly higher than that in control group (P<0.05). In 10-8 mol/L and 10-10 mol/L adenosine groups, there were no significant differences compared with control group (P>0.05). RT-PCR showed that in 10-4 mol/L and 10-6 mol/L adenosine groups, bFGF mRNA expression was higher than that in control group (P<0.05), while the difference between 10-8 mol/L adenosine group and control group was not significant (P>0.05). CONCLUSION: Adenosine may promote HUVEC proliferation and angiogenesis partly through inducing bFGF expression.     

基于分形信息维数的流域地貌形态与侵蚀产沙关系

流域地貌形态的科学准确量化是建立具有广泛适用性的流域尺度土壤侵蚀预报模型的关键科学问题之一，分形理论的提出为流域地貌形态特征的定量描述开辟了新的思路。本文基于分形理论，利用GIS技术和多元回归统计方法，以黄土高原丘陵沟壑区第一副区的岔巴沟流域为例，对该流域以分形信息维数为量化指标的流域地貌形态与降雨侵蚀产沙耦合关系进行了初步探讨。研究表明，引入地貌形态分形信息维数的岔巴沟流域降雨侵蚀产沙与地貌形态耦合关系模型可将岔巴沟各支流域差异明显的水沙关系简化为统一的数学表达，消除了由于地貌形态等下垫面因素导致的空间变异性；基于该耦合关系模型的西庄和蛇家沟流域次降雨输沙模数预测值和观测值相对误差较小、总体吻合较好；将地貌形态分形信息维数作为流域土壤侵蚀预报模型中地貌形态因子量化指标不仅可行，而且可靠。     

反刍动物埃立克体荧光定量PCR检测技术的建立和应用

为快速、准确地检测反刍动物埃立克体,本研究以反刍动物埃立克体pCS20为靶基因设计特异性引物和探针,建立了TaqMan和Eva Green荧光定量PCR方法,对其反应的特异性、敏感性和重复性进行了分析,并与OIE推荐的套式PCR方法一起对临床样品进行检测。结果显示,本方法特异性强,与牛巴贝斯虫、牛双芽巴贝斯虫、环形泰勒虫、犬埃立克体、牛埃立克体、马埃立克体和立氏埃立克体无交叉反应;TaqMan和Eva Green荧光定量PCR对pCS20质粒标准品的最低检测限分别为17.4拷贝·μL^-1和1.74拷贝·μL^-1,标准曲线相关系数大于0.99,组内和组间CV均小于1.5%。对420只钝眼蜱样本的检测显示,TaqMan和Eva Green荧光定量PCR的检出率分别为25.48%和29.29%,与套式PCR检测方法相比,敏感性更高。本研究为反刍动物埃立克体的检测和流行病学调查提供了一种快速、准确的检测方法。     

Nitric oxide production,NOS activity and expression in pulmonary arterioles of rats with chronic hypoxic hepercapnic pulmonary hypertension

AIM: To clarify the role of nitric oxide (NO) system in development of chronic hypoxic hypercapnic pulmonary hepertension. METHODS: Male Sprague-Dawley rats were randomly divided into control group and hypoxic hypercapnic group. NO content of plasma was determined, constitutive nitric oxide synthase (cNOS) and inducible nitric oxide synthase (iNOS) were examined using the technique of immunohistochemistry, expression of cNOS mRNA and iNOS mRNA of arteriole were detected by in situ hybridization. RESULTS: Plasma NO concentration, cNOS activity and cNOS mRNA expression in arteriole of chronic hypoxic hypecapnic group were significantly lower than that of control group (P<0.01); activity of iNOS and expression of iNOS mRNA in arteriole showed significantly higher compared with control. CONCLUSION: The disturbance of NO production and NOS expression in arteriole are involved in hypoxic hypercapnic pulmonary hepertension.     

施硒对大蒜产量和含硒量的影响

  以土壤施硒(0. 1 g·m^-2) 研究大蒜对硒的吸收、转化及硒对大蒜产量的影响。结果表明, 4 个大蒜品种吸收硒的能力为: 应县大蒜> 忻州大蒜> 榆次大蒜和太谷大蒜。以土壤施硒(0. 01、0. 1、1、10 g·m^-2) 、叶面施硒(10、100、1 000 mg·kg^-1) 和硒浸种10、100 mg·kg^-1) 3 种方式处理应县大蒜, 结果随着硒处理浓度的提高, 大蒜的产量先升后降,大蒜含硒量随着硒处理浓度的提高而提高, 但无机硒转化成有机硒的比率下降。