Characteristics in the secretion of liver TNF-α induced by infusion of LPS into veins in goats

AIM: To study the role of liver in immune regulation in experimental endotoxemia. METHODS: 17 castrated male goats were subjected to simultaneously installing catheters in jugular, hepatic and portal veins by surgery. Four days later, lipopolysaccharide (LPS) was infused in term of three groups as followings: In group ①, LPS of 20 EU (endotoxin unit, EU)·kg^-1 was infused into portal vein; In group ②, LPS of 20 EU·kg^-1 was infused into jugular vein and LPS of 1 500 EU·kg^-1 infused into jugular vein in group ③. Before and after infusion, blood samples were collected from the three veins through the catheters for 8 h.The plasma levels of TNF-α were measured by RIA. RESULTS: In group ①, the plasma TNF-α levels of hepatic and portal vein rose to peak value at 5 h, but that of the jugular vein did not changed. In group ②, the plasma TNF-α levels in hepatic vein rose to peak value at 3 h. The TNF-α levels of jugular vein rose to peak value at 1 h and the one in portal vein enhanced continuously between 0-8 h. In group ③, the plasma TNF-α levels in jugular, hepatic and portal vein rose to significant peaks at 1 h simultaneously. CONCLUSION: During experimental endotoxemia,liver showed different dynamic characteristics in TNF-α secretion according to the pathway and doses of LPS delivery.     

3,5-二硝基水杨酸法与酶法测定甜玉米还原糖和蔗糖含量的比较

针对目前测定甜玉米还原糖和蔗糖含量的3,5-二硝基水杨酸法和酶法进行比较研究,分析两者在育种中的应用。结果显示:酶法的测量准确度比3,5-二硝基水杨酸法高,且其稳定性和重现性都优于3,5-二硝基水杨酸法。通过两种方法对10个甜玉米杂交种种子的还原糖和蔗糖含量的测定,证实了两种方法在甜玉米还原糖和蔗糖含量的检测上是一致的。从育种实践考虑,酶法适于样品数较小的检测,3,5-二硝基水杨酸法更适合于大批量样品的检测。     

新型鸭呼肠孤病毒DH13株结构蛋白σB的原核表达及免疫原性分析

本研究旨在利用原核表达系统表达新型鸭呼肠孤病毒（NDRV） DH13株重组σB蛋白,并检测其免疫原性,为NDRV基因工程疫苗研制等提供物质材料。采用RT-PCR方法扩增获得NDRV σB基因,构建原核表达重组质粒pET-30a-σB和pET-32a-σB,将2个重组质粒转化大肠杆菌BL21（DE3）感受态细胞,利用IPTG诱导获得相应的重组蛋白,通过SDS-PAGE分析重组蛋白的表达形式。纯化无His的σB蛋白,并以此蛋白作为免疫原免疫新西兰白兔,制备兔源多克隆抗体。利用Ni²⁺柱亲和层析法纯化含His的σB蛋白,以此蛋白作为检测原,通过间接ELISA方法测定兔源多克隆抗体效价;Western blotting鉴定多克隆抗体与重组蛋白的特异性识别能力。结果显示,PCR扩增获得大小约为1 100 bp的σB基因片段。SDS-PAGE结果显示,高效表达出了2种重组σB蛋白,大小分别约为41和46 ku,均以包涵体形式存在。间接ELISA结果显示,制备的多克隆抗体效价达1∶204 800,能特异性地识别原核表达的重组蛋白,表明重组无His的σB蛋白具有良好的免疫原性。Western blotting特异性鉴定结果显示,含His的σB蛋白和无His的σB蛋白均与制备的兔源多克隆抗体发生特异性反应,表明原核表达得到的重组σB蛋白具备良好的反应原性。本试验利用原核表达系统成功地表达出了重组σB蛋白,并证实了重组NDRV σB蛋白具有良好的免疫原性及反应原性,该结果将有助于后续对NDRV σB蛋白生物学功能的鉴定、NDRV诊断用抗原的制备及NDRV新型疫苗的研制。     

佛山科技学院北院校园景观现状分析及改造构想

在实地调查的基础上，对校园景观现状进行总体分析，包括总体布局和空间组织、建筑景观、植物景观、水体景观、景观小品等方面，并对其景观改造设计提出初步构想。以期对我国高校建设中老校区改造设计有一定的借鉴意义。     

极香罗勒芳香油成分GC-MS分析

采用超临界CO2萃取技术,对极香罗勒芳香油进行了萃取,并利用气相色谱—质谱法(GC-MS)对芳香油成分进行了鉴定。结果表明:芳香油的主要成分为萜类物质共34种,占总芳香油的46.47%;脂肪酸类3种,占总芳香油的34.61%;芳香族醚类化合物有3种,总芳香油的7.84%;酯类有2种,占总芳香油的3.91%;烃类2种,占总芳香油的1.86%。芳香油中相对含量最高的是亚麻酸,占总芳香油的27.69%;其次是芳樟醇,占总芳香油的12.96%;其它含量较高的成分依次是(+)表-双环倍半水芹烯(7.53%)、m-丁香酚(5.88%)。     

药剂浸种对菜心增产效果的研究初报

采用OPAL HA202和“保得”对菜心进行浸种处理,可以促进菜心地上部分生长,增加干物质的积累,提早抽薹,有明显的增产作用,其中以OPAL HA202 200倍液处理增产效果最为显著。     

Experimental Pathological Study of Acute African Swine Fever

To study the clinicopathology and histopathology of African swine fever (ASF), and to explore the internal relationship between pathological changes and disease occurrence and development and its pathological mechanism, 13 Landrace pigs with bodyweight about 20 kg were intramuscular injected with African swine fever virus (ASFV), strain Pig/HLJ/18 at a dose of 10²HAD₅₀·mL^-1. During the experiment, all the dead pigs were systematically dissected and sampled, paraffin sections were produced, and haematoxylin-eosin staining was performed. Clinicopathological evaluation standards for acute ASF were established, then pathological lesions (classification variables) were expressed by counting frequency and percentage, and the lesion degree (continuous variables) was graded and scored according to different pathological changes of various tissues and organs. The results showed that all infected pigs were in line with the clinical characteristics of ASF, including acute, febrile and highly infectious, with a 100% incidence rate and 100% mortality. The dead pigs showed typical characteristics of septicemia, cadavers prone to corruption, blood clotting adverse or hemolysis, rigor mortis incomplete. The main pathological lesions were hemorrhagic necrotizing lymphadenitis, acute inflammatory splenomegaly (septic spleen), cerebral edema, pulmonary edema and lung consolidation et al. The spleen and lymphonodus are the target organs attacked by ASFV, with the most significant lesions, the earliest occurrence time, the longest duration and the highest frequency. The most prominent pathological changes are blood circulation disorders, including multiple pathological manifestations such as edema, hyperemia, congestion, hemorrhage, infarction, disseminated intravascular coagulation (DIC), and the most important characteristics are hemorrhagic lesions. The inflammatory reaction of lymphocytic exudation caused by ASFV runs through the whole process, especially in the middle and later stages of the course. The results suggest that the main pathological process of acute African swine fever is a typical immune/inflammatory cascade reaction and severe systemic blood circulation disorder, which resulted in the high incidence rate and high mortality rate of acute ASF.     

滇南小耳猪与巴马小型猪SLA-DQA基因多态分析

对滇南小耳猪和巴马小型猪的SLA-DQA基因的部分内含子1、完整的外显子2和部分内含子2共341bp片段进行了PCR-RFLP酶切分型,结果表明：2品种经EcoR Ⅰ酶切后BB基因型频率（0.468 8）高于AB型（0.375 0）,AA型最低（0.156 3）,B为优势基因（0.656 3）,A为劣势基因（0.343 7）.经AluⅠ酶切后,滇南小耳猪MM基因型频率（0.500 0）高于MN型（0.321 4）和NN型（0.178 6）;而巴马猪则以MN基因型频率（0.485 3）高于MM型（0.338 2）和NN型（0.176 5）;2个品种中M等位基因（0.604 2）频率高于N等位基因（0.395 8）.经分析表明,2猪种在两酶切位点上各分型已达Hardy-Weinberg平衡.巴马小型猪和滇南小耳猪中分别存在9种和7种PCR-RFLP组合基因型,其中BBMN为优势组合基因型,AAMN为劣势组合基因型;AAMM组合基因型在2品种间差异显著（P＜0.05）.遗传多态参数分析表明：SLA-DQA基因外显子2的两酶切位点在2猪种间均表现为中度多态,AluⅠ的基因多样性略高于EcoRⅠ,巴马小型猪杂合性略高于滇南小耳猪,2品种间遗传距离为0.000 4.     

广东部分地区中小型猪场猪伪狂犬病野毒感染的血清学调查

采用阻断ELISA法,对广东省9个地区经猪伪狂犬基因缺失苗免疫的中小型猪场2005年-2006上半年送检的375份血清进行猪伪狂犬病野毒感染的血清学检测。结果表明,有9个地区猪场血清呈阳性,其中阳性血清174份,平均阳性率为46.4%,最高阳性率达65.0%,提示该地区中小型猪场有猪伪狂犬病野毒感染。     

猪弓形体病的血清学调查

采用间接血凝试验(IHA)对广东省部分地区猪场送检的269份血清进行猪弓形体病的血清学检测,结果表明有61份血清阳性,平均阳性率为22.7%,最高阳性率为71.4%,该地区猪场有猪弓形体病感染。