浅论加强我国农村饮用水安全保护——基于法律保障的视角

近年来我国的水环境持续恶化,尤其农村的饮用水安全问题令人堪忧。文章从我国农村饮用水安全的现状以及存在的主要问题出发,着重探析造成现阶段我国农村饮用水安全种种问题的根源,基于法律保障的视角,注重法律制度诸项功能在具体操作层面上的充分发挥,指出我国农村饮用水安全保护的重要意义,提出合理的建议,希望能够对进一步探索和研究有所启示。     

凤县休闲农业发展现状及存在问题与对策

近年来,凤县转型发展休闲农业取得了巨大成功,对其发展历程进行深入研究,可以进一步促进凤县休闲农业规范快速发展,同时为我国休闲农业发展提供范例参考。详细阐述了凤县休闲农业发展的现状,对凤县发展休闲农业的优势进行了分析,就存在的问题重点从加强政府引导、理顺管理体制、制定规划标准及加大扶持力度4个方面阐明了凤县休闲农业发展的新举措,以期为凤县在休闲农业发展中获取更大的竞争优势提供参考。     

解决夏季母猪“饥而不饿”的营养招数

夏季来临，温度是影响猪群健康的第一要素，尤其对于母猪，在高温下其对营养的吸收利用及代谢均会发生改变，导致母猪代谢不畅而不愿采食，这将严重影响猪群健康及繁殖成绩。文章将从营养调节角度分析，并结合生产实践来解决这一问题，以期为猪场夏季母猪健康养殖提供借鉴和参考。     

Effect of eleutheroside B/E on proliferation and expression of PPARγ and TGF-β1 in HBZY-1 cells cultured with high glucose

AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression.     

Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.     

苗盘烟雾消毒方法对烤烟苗盘处理效果研究

为了验证苗盘烟雾消毒方法对烤烟苗盘的处理效果和安全性,试验设计了常规高锰酸钾消毒和苗盘烟雾消毒2种方法,结果表明:苗盘烟雾消毒方法省工省时,药剂用量和耗水量少,对烤烟苗盘有着较好的消毒效果,且对烟苗生长没有负面作用。     

荷斯坦犊牛日粮蛋白质消化代谢的研究

本文旨在研究荷斯坦犊牛日粮蛋白质消化模式的转变、转化效率和代谢特点。选用3头2日龄的荷斯坦公犊进行消化试验和氮平衡试验。试验结果表明,在犊牛1~6月龄期间氮的消化率平均为70.39±6.86,氮的生物学价值平均为51.31±6.04,氮的总利用率平均为36.48±7.24。犊牛日沉积氮量20.80g,每千克增重需氮量44.26g,或日沉积蛋白质130g,每千克增重需蛋白质量279.94g,小于成年牛的需求。