带有GFP的小鼠Sox2基因逆转录病毒载体的构建及其检测

为了建立体细胞重编程过程中检测Sox2基因表达变化的方法,本研究利用克隆获得的小鼠Sox2基因与逆转录病毒载体pMX—IRES—GFP连接,构建带有报告基因GFP的逆转录病毒载体,将其转染293GP细胞后获得假病毒上清。将获得假病毒上清侵染小鼠成纤维细胞（NIH3T3细胞）后观察GFP的表达和检测Sox2转录量的变化。其结果,成功构建带有GFP的Sox2基因逆转录病毒载体pMX—Sox2-IRES—GFP;将构建的载体转染后293细胞后获得能侵染NIH3T3细胞的假病毒上清,且与未侵染的NIH3T3细胞相比,侵染后的NIH3T3细胞的Sox2表达量提高近10倍。本研究为开展在体细胞重编程过程中Sox2表达与重编程效率的相关性提供依据．     

复合氧化法降解染料活性艳蓝P-3R的研究

以配制的活性艳蓝P-3R染料废水为研究对象,研究了复合氧化法O3/H2O2降解染料活性艳蓝P-3R的效果,并分析了反应时间、H2O2投加量、H2O2投加方式、pH值等各种因素对处理效果的影响。结果表明:O3/H2O2比单独O3反应的效果要好;反应时间越长,脱色率和TOC去除率也越大;H2O2投加量对氧化反应的影响很大,存在一个最佳投加量;总量相同的H2O2一次投加优于多次投加;pH值越大,去除率也越大;Na2CO3作为自由基清除剂在一定程度上抑制了O3/H2O2氧化。     

发展新能源汽车及其强化节能减排的思考

指出了大力推动新能源汽车发展将是我国汽车工业的大势所趋,分析了发展新能源汽车的原因、挑战和方向,探讨了发展新能源汽车对推进节能减排事业的意义。     

The role of toll-like receptors in the pathogenesis of Streptococcus suis

Streptococcus suis is an important agent of swine and human meningitis. Sequence type (ST) 7 emerged in China and was responsible for the human epidemic caused by S. suis in 2005. The virulence of S. suis ST7 is greater than the wild type pathogenic S. suis, ST1; however, the mechanisms for this increased pathogenicity are unknown. The aim of this study was to determine the role of different toll-like receptors (TLRs) involved in regulating the host response to the S. suis infection and to speculate on differing mechanisms used by ST7 strains to induce disease. Here we compared two ST7 strains isolated in the 2005 Sichuan outbreak to two ST1 strains. Our data show TLR2, 6 and 9 are involved in the recognition of heat-killed S. suis independent of the ST type. We found the TLR-dependent cytokine production differed between the two types of strains using whole cell lysate proteins. TLR6 played a greater role in cytokine production induced by the whole cell lysate proteins from the ST7 strain than in that induced by the ST1 strain lysates. The data suggest that mechanisms of inflammation induced by S. suis strains differ where this will be useful in designing efficient strategies in combating streptococcal toxic shock-like syndrome caused by the S. suis ST7 strains.     

毛竹低产低效林的经营策略研究

毛竹是南方竹产区山区农户主要经济收入来源和当地农业经济的支柱产业之一.当前,毛竹低产低效林面积依然很大,占竹林总面积的60％左右.该文以农户访谈和二手资料收集等方法,了解掌握毛竹低产低效林改造的技术状况和运行机制,从毛竹培育技术、农户对技术采用和扩散的特征分析出发,提出了毛竹低产低效林的经营策略建议.     

Sesquiterpenoids from Carpesium divaricatum and their cytotoxic activity

In the course of searching for cytotoxic terpenoids from medicinal plants in China, two new eudesmane sesquiterpenoids, 5α-hydroxy-13-methoxy-7αH,11αH-eudesm-4(15)-en-12,8β-lactone (1) and 1β-hydroxy-7αH,11αH-eudesm-4(15)-en-12,8β-lactone (2), along with fourteen known sesquiterpenoids were isolated from the whole plant of Carpesium divaricatum. The structures of new compounds were determined using spectroscopic methods, including IR, HRESIMS, and 1D and 2D NMR spectroscopy. The cytotoxicity of selected sesquiterpene lactones against human oral epidermoid carcinoma (KB), human breast cancer (MCF-7) and human hepatoma (HepG-2) cells was also evaluated by MTT method.     

饲料营养价值评定方法进展

饲料营养价值评定是动物营养研究的基本方法手段,也是建立饲料数据库,为工业饲料产品的研发和生产、畜禽养殖提供基本参数的主要手段。随着分析检验和动物试验设备装备水平的提高、试验方法的进步,饲料营养价值评定方法得到了进一步的改进。文章对饲料营养价值评定采用的新方法与传统方法进行综述。     

石河子垦区猪场五种病原混合感染的血清学调查

为了解石河子垦区规模猪场猪圆环病毒2型(PCV-2)与猪肺炎支原体(Mhp)、胸膜肺炎放线杆菌(App)、副猪嗜血杆菌(Hps)、猪链球菌2型(SS2)混合感染情况,采集垦区16个猪场420份血清进行了PCV-2与Mhp、App、Hps、SS2混合感染的血清学调查.结果显示,猪场PCV-2感染率达100％ (16/16),PCV-2感染阳性率为80.95％(340/420),PCV-2与Mhp、App、Hps、SS2双重混合感染率分别达53.82％(183/340)、43.82％(149/340)、64.42％(219/340)、45.1％(158/340);三重混合感染率为15％～36.47％,四重混合感染为13.24％～25.88％,五重混合感染为10.59％.调查结果表明垦区规模猪场PCV-2感染及其与Mhp、App、Hps、SS2混合感染情况普遍存在.     

酵母多糖对环磷酰胺所致免疫损伤大鼠的拮抗作用

为研究酵母多糖(YP)对环磷酰胺(CTX)所致免疫损伤大鼠的拮抗作用,本实验将80只雄性SD大鼠随机分为YP(50 mg/kg)+CTX组、YP(100 mg/kg)+CTX组、YP(200 mg/kg)+CTX组、CTX对照组和正常对照组。YP+CTX组按剂量灌胃并称重,CTX和正常对照组则灌胃给予等量生理盐水,连续给药10 d;在第8 d、9 d,除正常对照组外,其余4组腹腔注射CTX 100 mg/kg。第11 d采血及对相关的免疫器官组织进行检测。结果显示,YP(100 mg/kg)组平均日增重和饲料报酬比正常对照组显著增加(p<0.05);胸腺指数,血清中IgA、IgG、表皮生长因子(EGF)、碱性磷酸酶(AKP)含量及空肠SIgA水平显著(p<0.05)或极显著(p<0.01)高于CTX对照组;结肠壁中前列腺素E2(PGE2)含量与CTX对照组相比显著降低(p<0.05)。以上结果表明,YP能提高大鼠的生长性能;YP对CTX所致免疫损伤具有一定的拮抗保护作用,其中100 mg/kg剂量的YP效果最为显著。