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121.
实验性慢性氟中毒家兔肝、肾动态超微病理学研究 总被引:1,自引:0,他引:1
24只20~30日龄体重0.7~1.2kg新西兰白兔随机分为实验组15只(每日每只随饮水摄取NaF45mg/kg体重)及健康对照组9只(饮用自来水).两组在相同饲养管理条件下实验观察.分别在实验第30,60.90d时迫杀,对肝和肾进行超微病理学研究.结果表明,肝和肾实质细胞膜系统损伤明显,胶原原纤维再生增多,且有再生障碍及再生后的损伤性变化. 相似文献
122.
Olivier M Aggarwal A Allen J Almendras AA Bajorek ES Beasley EM Brady SD Bushard JM Bustos VI Chu A Chung TR De Witte A Denys ME Dominguez R Fang NY Foster BD Freudenberg RW Hadley D Hamilton LR Jeffrey TJ Kelly L Lazzeroni L Levy MR Lewis SC Liu X Lopez FJ Louie B Marquis JP Martinez RA Matsuura MK Misherghi NS Norton JA Olshen A Perkins SM Perou AJ Piercy C Piercy M Qin F Reif T Sheppard K Shokoohi V Smick GA Sun WL Stewart EA Fernando J Tejeda Tran NM Trejo T Vo NT Yan SC Zierten DL Zhao S 《Science (New York, N.Y.)》2001,291(5507):1298-1302
We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases. 相似文献
123.
粗糙集神经网络在昆虫总科阶元分类学上的应用 总被引:2,自引:0,他引:2
为研究粗糙集和神经网络相结合方法的实践性,以及昆虫的数学形态特征在总科阶元上作为分类特征的可行性、可靠性和重要性。从总科角度对鳞翅目(Lepidoptera)和鞘翅目(Coleoptera)5个总科23种虫体图像中提取的昆虫面积、周长等11项数学形态特征进行粗糙集神经网络分析。结果表明:在总科阶元上,11项特征的可靠性大小为,面积、亮斑数>周长、横轴长、形状参数、圆形性、似圆度、偏心率>纵轴长、叶状性、球状性,与赵汗青等的统计分析结果中属性特征的重要性大多数一致;神经网络模式识别结果与传统分类结果完全一致。应用粗糙集理论进行昆虫数学形态特征分类结果准确;在昆虫总科阶元分类上粗糙集神经网络较统计学方法具有优势。 相似文献
124.
培养条件对嗜水气单胞菌胞外蛋白酶合成与分泌的影响 总被引:5,自引:0,他引:5
对体外培养嗜水气单胞菌的胞外蛋白酶 (ECPase)产生条件进行了优化。结果发现 ,其产量与碳源、氮源、培养温度、培养基初始pH值等有关。蔗糖能促进蛋白酶的产生 ,NH4 + 抑制蛋白酶的产生。其最佳产酶条件为 :pH 7 5的0 0 2mol·L-1KC1,0 0 6mol·L-1K2 HPO4 ,5g·L-1的蔗糖和 5g·L-1的胰蛋白胨水 ,2 8℃ ,15 0r·min-1摇床培养 6 5h。在此条件下酶活性可达 2 0 8U·mL-1。 相似文献
125.
Ferguson AD McKeever BM Xu S Wisniewski D Miller DK Yamin TT Spencer RH Chu L Ujjainwalla F Cunningham BR Evans JF Becker JW 《Science (New York, N.Y.)》2007,317(5837):510-512
Leukotrienes are proinflammatory products of arachidonic acid oxidation by 5-lipoxygenase that have been shown to be involved in respiratory and cardiovascular diseases. The integral membrane protein FLAP is essential for leukotriene biosynthesis. We describe the x-ray crystal structures of human FLAP in complex with two leukotriene biosynthesis inhibitors at 4.0 and 4.2 angstrom resolution, respectively. The structures show that inhibitors bind in membrane-embedded pockets of FLAP, which suggests how these inhibitors prevent arachidonic acid from binding to FLAP and subsequently being transferred to 5-lipoxygenase, thereby preventing leukotriene biosynthesis. This structural information provides a platform for the development of therapeutics for respiratory and cardiovascular diseases. 相似文献
126.
Chu FL Pirastru L Popovic R Sleno L 《Journal of agricultural and food chemistry》2011,59(7):3004-3013
Carotenoids have potent antioxidant activity as well as therapeutic value, and their formation has been seen to be induced in algae by stress, including high-salt culture conditions. A differential profiling of carotenoids was conducted using a targeted metabolomics approach with accurate mass data generated by liquid chromatography-electrospray-time-of-flight (LC-ESI-TOF) mass spectrometry followed by postacquisition filtering based on isotope patterns and mass defects to detect carotenoids up-regulated in Scenedesmus sp. exposed to high-salt conditions. Algal cultures treated with high concentrations of sodium acetate or sodium chloride were found to cause an increase in various carotenoids. On the basis of differential analysis, astaxanthin and canthaxanthin increased upon salt treatment. Astaxanthin, in its free form and as fatty acid esters, was seen to increase in Scenedesmus sp. using accurate mass MS. A few other carotenoid compounds increased upon salt treatment, including echinenone and adonirubin, involved in the pathway of astaxanthin biosynthesis from β-carotene, as well as isomers of astaxanthin and canthaxanthin. A time course study of acetate treatment was done to observe the time-dependent up-regulation of carotenogenesis. 相似文献
127.
猪粪麦秆不同比例混合厌氧发酵特性试验 总被引:12,自引:0,他引:12
以猪粪、麦秆为原料,研究了35℃下二者按不同比例混合对厌氧消化产沼气的影响,分析了消化过程中日产气量、累积产气量、甲烷含量、原料去除率、pH值以及氨态氮质量浓度的变化。结果表明,猪粪与麦秆配比(干物质量比)1∶1时产气量最大,为383.0mL/g,是麦秆单独发酵产气量(231.8mL/g)的1.6倍;混合原料(猪粪和麦秆配比分别为1∶1、2∶1、3∶1)的VS去除率均在37%以上,比麦秆提高12.0%~26.9%;添加猪粪可提高发酵液中氨态氮含量,较麦秆提高35.6%~64.8%。因此,合理调控粪秆混合厌氧发酵的比例,能提高秸秆的产气率和利用率。 相似文献
128.
基于雷诺时均Navier-Stokes方程和标准k-ε湍流模型,利用Fluent软件对文丘里施肥器的内部流动进行了数值模拟,并对数值计算方法的正确性进行了验证.模拟分析了喉管进口直径为4,5,6 mm,以及出口直径与进口直径之比λ为1.0,1.1,1.2,1.3和1.4时15种结构方案的吸肥性能.结果表明:喉管直径对喉管及扩散段内流动特性、吸肥性能和效率均有一定影响,但出口直径与进口直径之比λ对文丘里施肥器性能的影响要明显甚于喉管进口直径.根据效率最高原则,喉管直径比应为1.2~1.3.建立了λ=1.2或1.3时文丘里施肥器吸肥效率与流量比之间的回归模型,并应用回归模型预测了吸肥效率最大值.喉管进口直径4 mm的文丘里施肥器当直径比为1.3时吸肥效率可达到15.5%,与直径比为1.0相比,前者的扩散段内部流动平顺、无漩涡出现,且相同横截面的平均湍动能值要小.该研究结果为文丘里施肥器的优化设计提供了一定的理论参考. 相似文献
129.
本研究利用217个微卫星标记和336个SNPs标记对德国镜鲤F2代68个个体基因组DNA进行基因型检测。其中507个标记共组成62个连锁群,覆盖基因组总长度为2805.85cM,标记问平均距离为6-31cM;利用软件MapQTL4.0采用区间作图法对体重性状进行QTL定位分析。研究结果共检测到14个与体重性状有关的QTLs,分布于9个连锁群。其中BIV-5-J有最大的LOD值,为4.46;BIV—I-1的LOD值最小,为2.25。单个QTL平均解释表型变异介于14.10%~45.50%之间,其中贡献率大于20%的主效QTLs有9个。通过BLASTX与斑马鱼蛋白质序列数据库进行序列比对,找到了与斑马鱼酰基辅酶A脱氢酶蛋白、胰淀粉酶仅2蛋白、Apoebprotein和甘油醛-3-磷酸脱氢酶蛋白同源的分子标记。本研究结果对分子标记辅助育种具有重要应用价值。 相似文献
130.
Lin TY Chen CY Chien SC Hsiao WW Chu FH Li WH Lin CC Shaw JF Wang SY 《Journal of agricultural and food chemistry》2011,59(14):7626-7635
Antrodia cinnamomea is a precious edible fungus endemic to Taiwan that has long been used as a folk remedy for health promotion and for treating various diseases. In this study, an index of 13 representative metabolites from the ethanol extract of A. cinnamomea fruiting body was established for use in quality evaluation. Most of the index compounds selected, particularly the ergostane-type triterpenoids and polyacetylenes, possess good anti-inflammation activity. A comparison of the metabolite profiles of different ethanol extracts from A. cinnamomea strains showed silmilar metabolites when the strains were grown on the original host wood (Cinnamomum kanehirai) and harvested after the same culture time period (9 months). Furthermore, the amounts of typical ergostane-type triterpenoids in A. cinnamomea increased with culture age. Culture substrates also influenced metabolite synthesis; with the same culture age, A. cinnamomea grown on the original host wood produced a richer array of metabolites than A. cinnamomea cultured on other wood species. We conclude that analysis of a fixed group of compounds including triterpenoids, benzolics, and polyacetylenes constitutes a suitable, reliable system to evaluate the quality of ethanol extract from A. cinnamomea fruiting bodies. The evaluation system established in this study may provide a platform for analysis of the products of A. cinnamomea. 相似文献