施肥和灌溉对蔬菜品质影响的研究进展

综述了蔬菜品质的内涵及施肥、灌溉对蔬菜品质影响的研究进展。综合前人研究结果可以看出,适量增加施肥量通常可改善蔬菜商品品质,并可提高蔬菜产品中蛋白质、游离氨基酸、有机酸等营养物质指标的含量,但过量施肥则常导致总糖、可溶性糖、抗坏血酸等营养成分含量的降低,并可导致卫生品质及风味品质的降低。合理灌溉可以有效提高蔬菜各种品质指标,但对于许多果菜类而言,适当控制浇水量则有利于营养品质的提高。通常情况下,施肥与灌溉对蔬菜产品品质的影响结果受土壤基础肥力、肥料种类及其配比、蔬菜种类、产品成熟度等因素的影响较大。     

Ca~(2+)-ATPase抑制剂对采后草莓呼吸速率和花青苷含量的影响

用Ca2+-ATPase抑制剂钒酸钠(SO)和曙红(EB)浸泡处理转红期草莓果实(cv.Darselect)后表明,2者能提高果实呼吸速率,迅速促进外层果肉的花青苷生成,增加其可溶性糖含量,髓部果肉的花青苷生成和可溶性糖的变化较慢,而对果实的失重率影响不大。因此,Ca2+-ATPase抑制剂具有促进果实成熟衰老的作用。     

套袋梨园病虫害综合防治

套袋梨园应侧重于对枝干、叶部病虫害及人袋害虫的防治。选择高效、低毒、低残留、低药害的农药种类,同时要避免用药种类单一,以防病虫产生抗药性。在防治方法上,应坚持“综合防治”,本着“预防为主,治疗为辅”的原则。要注意保护天敌,选用对天敌无害的生物农药,在天敌发生高峰期不用药。强化农业措施防治、生物防治和人工防治。注意“三个结合”,即杀菌剂和杀虫剂相结合,防病虫药剂与叶面施肥相结合,药剂与助剂(多功能植物增效剂、展着剂等)相结合。     

淮北地区荷兰豆高产栽培技术要点

荷兰豆系采收嫩荚的食用荚豌豆,风味独特,色泽鲜绿,深受国际市场的欢迎.我地从1992年开始引进试种,现已形成近2万亩的生产规模.经技术人员的不断探索,已掌握一套行之有效适宜淮北地区的高产栽培技术,现将有关技术要点介绍如下：     

Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

早熟大果鲜食枣品种‘七月鲜''

‘七月鲜’是从合阳县农家品种中选出的早熟优良品种，比中熟品种‘梨枣’早熟20d以上；果大，整齐，平均单果质量29．8g，可溶性固形物含量28．9％，可食率97．8％，味甜，肉质细，极宜鲜食，不易裂果，早果性强，抗缩果病，节间短，树体矮小，适宜矮化密植和设施栽培。     

Cloning of p15INK4b related gene CCRG and expression analysis in the renal carcinoma

AIM and METHODS:To analysis the factor that involved in renal carcinogenesis, we used the bait gene AK001518 to screen GenBank. To understand the relationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Northern Blot experiments. Then we examined CCRG expression level in renal carcinogenesis. RESULTS:Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK001518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically decreased when p15 gene was over-expressed. CCRG expression level was much higher in tumor tissues and cells than normal tissues and cells. CONCLUSION:The novel gene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.     

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.     

番木瓜环斑病毒株系的分子生物学方法鉴定

 以PRSV株系特异性引物对PRSV的PRSV126(PRSV日本分离物)、Ys、Vb和Sm等株系进行RT-PCR方法鉴定,引物PR21/PR22能把Ys从Vb和Sm中鉴定出来,PR300/PR301则能把Vb从Ys、Sm和PRSV126中鉴定出来;用限制性内切酶Hae Ⅱ、Sau3A I和Hinf I对PRSV的PRSV126、Ys、Vb和Sm等株系进行单酶切RT-PCR-RFLP分析,Hinf I能把PRSV126与Ys、Vb和Sm鉴别开来,Sau3A I能把Ys与Vb和Sm鉴别开来,Hae Ⅱ则能把Ys与PRSV126、Vb和Sm鉴别开来;以P1/P2为引物,对Vb、Ys和Sm株系进行RT-PCR-RFLP-SSCP分析,结果能一次把三者较好地区别开来。