棉花铃重AFLP标记的初步研究

本文以陆地棉铃重近等基因系为材料(大铃≥5.4g,小铃≤1.7g),构建大小铃两个近等基因池.利用AFLP标记技术对两基因池进行分析,64对引物组合共产生3840条稳定、清晰扩增带.五条扩增带在两基因池间呈现多态性,其中四条差异带出现在大铃基因池,编号分别为LB-1、LB-2、LB-3和LB-4;一条出现在小铃基因池,编号为SB-1.模板浓度梯度分析表明,在一定范围内,酶切模板浓度对AFLP结果影响较小.利用稍作修改的CTAB法提取的棉花DNA可用于AFLP分析.     

伊犁马体尺年龄对其竞赛性能影响的研究

对伊梨马体尺、年龄与不同赛程成绩关系的研究表明:体高对各赛程影响极显著(P<0.01),但随赛程增长,影响逐渐减弱;胸围和管 围对各赛程影响显著(P<0.05);年龄对长赛程影响显著(P<0.05)。     

高盐环境下Cr(VI)/亚硫酸盐体系氧化降解效能研究

鉴于亚硫酸盐处理含Cr(VI)废水时会产生氧化性极强的硫酸根自由基和羟基自由基，使得亚硫酸盐的消耗量增加，为提高亚硫酸盐的利用率并将产生的活性自由基加以利用，选取了常见的偶氮染料酸性橙7(AO7)作为目标有机污染物用以捕获产生的活性自由基，以达到含Cr(VI)废水和有机污染废水协同处理的目的。通过紫外可见吸收光谱法和显色法测定AO7、Cr(VI)和亚硫酸盐等各反应物的浓度变化，采用自由基捕获方法鉴定反应中的活性氧化物种。结果表明，Cr(VI)/亚硫酸盐体系能够在降解AO7的同时，将Cr(VI)转化为Cr(III), S(IV)氧化为硫酸根离子。在[Na2SO3]0=0.5 mM、[Cr6+]0=0.1 mM、pH=3.0条件下，Cr(VI)/亚硫酸盐体系对AO7的降解效果最好，反应60 min后，AO7降解率和Cr(VI)的还原率分别为86.1%和82.0%，且氯离子的加入几乎无影响。在反应体系中加入0.1 mM乙醇或叔丁醇后，AO7的降解受到不同程度影响，在Cl?= 0/300 mM条件下，AO7的降解率分别变为56.6%/71.7%和80.7%/81.5%，证明该体系中的主要氧化物为SO4?? 和?OH，且起主要作用的是SO4??。AO7降解实验、可吸附卤代物(AOX)和气相色谱-质谱联用(GC-MS)结果表明，Cr(VI)/亚硫酸盐体系在高盐环境下能保持较高的降解效率，且不会增加氯代二次产物的生成，这种新型的氧化降解体系有望在高盐工业废水处理中得到应用。     

自由基化学改性氧化石墨烯对亚甲基蓝的吸附性能

氧化石墨烯（Graphene Oxide,GO）拥有较大的比表面积和较高的稳定性，可用来吸附水体中的多种污染物，其潜在功能稳定性具有规模化应用前景。考虑到GO在高级氧化体系中结构和形貌会发生改变，探究了经UV/H2O2和UV/过硫酸盐(Persulfate, PS)产生强氧化性自由基的体系处理后所得GOs对亚甲基蓝(Methylene Blue, MB)的吸附性能。氧化性自由基体系光源为300 W中压汞灯，30 mL GO储备液(1 mg/mL)在2种氧化体系下反应1、2、4 h后制得GOs。实验考察了不同反应条件对GOs吸附动力学的影响，傅里叶变换红外光谱(FTIR)研究了GOs氧化前后表面官能团的变化。结果显示，经UV/H2O2, UV/PS氧化1 h后，GOs表面的含氧官能团数量开始明显减少；吸附动力学过程更符合准二级动力学模型（R2>0.999）；吸附热力学过程更符合Langmuir模型，MB饱和吸附量(经UV/H2O2和UV/PS氧化1、2、4 h后分别记G1-1, G1-2, G1-3, G2-1, G2-2, G2-3)依次为580.26, 591.80, 598.63, 521.77, 554.91, 568.00 mg/g。研究表明，GO可以快速吸附较大浓度范围内的MB，且对低浓度染料表现出更好的去除效果；GO对MB的吸附量随着pH值的增加逐渐增大，且在碱性条件下的增加得更明显；经氧化处理后，GOs的吸附性能随着氧化时间的增长，性能减弱，尤其在UV/PS体系中更为显著。     

Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1

AIM: To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS: The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS: Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION: Compared to independent culture, ASC reduces the resting ROS production by co-cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.     

Effects of diabetes on the development of heart failure in streptozotocin-induced diabetic rat model with acute myocardial infarction

AIM: To evaluate the time course and effect of diabetes on the development of heart failure (HF) in poorly controlled streptozotocin (STZ) -induced diabetic rat model for 70 d with acute myocardial infarction (AMI) in vivo. METHODS: All SD rats were randomized into four groups. Diabetes were induced by a single intraperitoneal injection of STZ (65 mg/kg), and 70 d later after the induction, AMI models were made with the ligation of left anterior descending coronary artery. The time course of diabetic effects on the development of heart failure in rats before and after AMI was observed. The survival rate of the rats and ultrastructure change of myocardium, the hemodynamics, the extent of the myocardial fibrosis, and the cardiac hypertrophy were also determined. RESULTS: After the ligation of left anterior descending coronary artery, the diabetic rats showed worse LV function and accelerated left ventricular (LV) remodeling compared with the non-diabetic ones. Myocardial fibrosis in both diabetic and non-diabetic rats subjected to AMI was similar in the early phase, while it was quite different after 1 month. CONCLUSION: Heart failure progression is accelerated in diabetic rat with AMI.     

唐山市板栗红蜘蛛防治试验

板栗红蜘蛛是唐山市板栗生产上的主要害虫.根据板栗红蜘蛛发生消长规律,采用烟雾机喷雾施药,研究了聚乙烯醇、刹死倍和3种杀螨剂对红蜘蛛越冬卵和发生期螨的防治效果.结果表明:对越冬卵的防效聚乙烯醇40倍液+5度石硫合剂在栗树休眠期可达86.53%;刹死倍200倍液在栗树芽萌动期达77.9%;尼索朗对发生期螨的防效达80.6%,均可在板栗产区推广应用.     

高校信息化建设和管理模式的探讨

本文针对高校信息化建设和管理中存在的问题以及笔者在高校信息化建设和管理中的亲身感受,在认真思考的基础上,提出了建设和管理统一化的设想.