白屈菜CmFAD2基因的克隆及植物转化载体构建

通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正"V"型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。     

校园环境噪声监测与评价--以华中师范大学为例

环境噪声是环境质量评价的重要指标。通过噪声监测实验,记录了华中师范大学校园内的噪声污染数据,根据国家标准评估了校园环境噪声状况。试验采用网络剖分法,分别于昼间7:30~10:00和夜间22:00~22:55两个时间段对等效声级进行了测定。实验结果表明:昼间噪声污染更为严重,平均噪声超标率达到26%,夜间噪声污染相对较轻,但噪声超标率也达到19%。校园内不同功能区的噪声来源不同,工程施工产生的噪声是主要的污染源,影响比重达到58%。根据实验结果,校园声环境质量一般,需要对现状提出合理的改进建议。     

京津唐早熟区夏玉米品种推广现状及育种思考

京津唐区位于东华北春玉米区和黄淮海夏玉米区之间,由于气候的特殊性,玉米品种类型相对单一;现在越来越多的公司关注该生态区玉米生产推广现状和玉米品种选育情况。对该区域的气候特殊性和生产主推品种进行了简单归纳总结,为该区域的新品种选育和推广作参考。     

贵州省食用菌产业现状与可持续性发展分析

笔者分析了贵州省食用菌产业特点与产业优势,认为贵州食用菌产业在自然条件、交通条件以及政策条件等方面均具有较大优势;全省食用菌规模逐渐扩大,品牌效应凸显。进一步总结了贵州省食用菌产业存在的问题,即缺乏行业规范,产品结构较为单一,行业人才匮乏。继而提出了贵州省食用菌产业可持续性发展建议,即加大食用菌产业的开发程度,加强科技支撑及专业人才培养,政府统筹,建立行业标准,重视菌渣的综合开发利用,以实现本省食用菌产业的后发赶超和可持续性发展。     

气吸式覆膜精量油菜播种机排种性能试验与优化设计

针对目前高寒地油菜播种机普遍存在的重播漏播率高、人工劳动强度大、生长期缺水等问题，研究设计了一种气吸式精量播种机，一次性完成膜上播种、施肥、滴水灌溉、覆膜。分析了播种机主要结构、工作原理，对其关键部件进行结构设计，以排种盘转速、气吸室负压、排种盘型孔直径为试验因素，利用软件优化分析获得合理参数组合。结果表明：播种机排种盘转速197 r·min^-1、气吸室负压2.5 kPa、排种盘型孔直径1.2 mm时，田间播种合格率为93%、重播率3%、漏播率1%，播种质量较好。田间试验表明上述参数组合下排种器工作性能满足油菜播种机工作性能要求，该研究为油菜精密播种机的研究和设计提供了理论和技术参数。     

The M43 domain-containing metalloprotease RcMEP1 in Rhizoctonia cerealis is a pathogenicity factor during the fungus infection to wheat

Wheat(Triticum aestivum L.) is an important staple crop for global human. The necrotrophic fungus Rhizoctonia cerealis is the causal pathogen of sharp eyespot, a devastating disease of wheat. Herein, we identified RcMEP1, a zinc metalloproteaseencoding gene from R. cerealis genomic sequences, and characterized its pathogenesis function. RcMEP1 expressed at markedly-high levels during R. cerealis infection process to wheat. The predicted protein RcMEP1 comprises of 287 amino acid residues and contains a signal peptide and a M43 metalloprotease domain harboring the active site motif(HEVGHWLGLYH). The assays of Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana leaves indicated that RcMEP1 is an apoplastic elicitor of cell death, and that the predicted signal peptide functions and is required for secretion and cell death-induction. The purified RcMEP1 protein and its M43 domain peptide were individually able to induce plant cell death and H2 O2 accumulation, and to inhibit expression of host chitinases when infiltrated into wheat and N. benthamiana leaves, while the M43 domain-deleting peptide and negative control lacked the capacity. Moreover, compared with the control pretreatment, the purified RcMEP1 protein or its M43-domain peptide resulted in enhanced pathogenesis in the inoculated wheat, whereas the M43 domain-deleting peptide failed. These results suggest that RcMEP1 acted as an important pathogenicity factor during R. cerealis infection to wheat and that its signal peptide and M43 domain are required for the secretion and pathogenesis of RcMEP1. This study provides insights into pathogenesis role of M43 domain-containing metalloproteases during R. cerealis infection to wheat.     

Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.     

IWOS系统在配网故障处理中的应用

随着我国经济社会的不断发展,全社会用电量不断上升,对电网的可靠性、安全性和电能质量也提出越来越高的要求。由于历史原因,配网建设欠账较多,总体基础薄弱,发展落后于主网,区域、城乡之间发展不均衡,水平差异大,存在明显短板。随着电动汽车、分布式电源、微电网、储能装置的快速发展,对配电网的安全性、经济性、互动性、适应性提出越来越高的要求。在国家电网公司建设“大运行”体系、推进地调县调一体化进程背景下,建设安全智能的配网自动化系统成为当务之急,但现有配网线路大多存在着技术、设备落后等问题,自动化水平不高,难以直接建成大规模的配网自动化系统。     

Effect of dietary calcium or phosphorus deficiency on bone development and related calcium or phosphorus metabolic utilization parameters of broilers from 22 to 42 days of age

This experiment was conducted to investigate the effect of dietary calcium (Ca) or phosphorus (P) deficiency on bone development and related Ca or P metabolic utilization parameters of broilers from 22 to 42 days of age based on our previous study, which indicated that dietary Ca or P deficiency impaired the bone development by regulating related Ca or P metabolic utilization parameters of broilers from 1 to 21 days of age. A total of 504 one-day-old Arbor Acres male broilers were randomly assigned to 1 of 4 treatments with 7 replicates in a completely randomized design, and fed the normal control and Ca- or P-deficient diets from 1 to 21 days of age. At 22 days of age, the broilers were further fed the normal control diet (0.90% Ca+0.35% non-phytate P (NPP)), the P-deficient diet (0.90% Ca+0.18% NPP), the Ca-deficient diet (0.30% Ca+0.35% NPP) or the Ca and P-deficient diet (0.30% Ca+0.18% NPP), respectively. The results showed that dietary Ca or P deficiency decreased (P<0.05) tibia bone mineral density (BMD), bone breaking strength (BBS), ash content, tibia ash Ca content and serum P content on days 28 and 42, but increased (P<0.05) tibia alkaline phosphatase (ALP) activity of broilers on day 42 compared with the control group. Furthermore, the broilers fed the P-deficient diet had the lowest (P<0.05) tibia BMD, BBS, ash content, serum P content and the highest (P<0.05) serum Ca content on day 28 compared with those fed the Ca-deficient or Ca and P-deficient diets. The results from the present study indicated that the bone development and related Ca or P metabolic utilization parameters of broilers were the most sensitive to dietary P deficiency, followed by dietary Ca deficiency or Ca and P-deficiency; dietary Ca or P deficiency impaired the bone development possibly by regulating serum Ca and P contents as well as tibia Ca content and ALP activity of broilers from 22 to 42 days of age.