MlNAC2基因的克隆及其在南荻根部的表达模式

克隆南荻MINAC2基因的c DNA序列,该序列全长为933 bp,编码产物含310个氨基酸残基,其蛋白质理论相对分子质量为34 427.8,等电点(p I)为5.85,不稳定系数为34.84,为稳定蛋白,具有保守的NAM基序,无信号肽和跨膜结构域,可推测其为亲水性蛋白。亚细胞定位试验结果表明,Ml NAC2定位于细胞核,可能在细胞核内行使功能。转录激活试验结果表明,Ml NAC2是一个转录因子蛋白,且转录激活域位于C端。荧光实时定量PCR分析结果表明,高盐、干旱、脱落酸、茉莉酸甲酯和机械伤害均能诱导Ml NAC2基因在南荻根部的表达上调,而低温处理时表达下调。     

土壤速效氮、磷、钾含量空间变异特征及其影响因子

利用地统计学和GIS相结合的方法,探讨了双流县土壤速效氮、磷、钾含量空间变异特征及其影响因子。结果表明:1) 土壤速效氮和速效钾含量具有强烈的空间相关性,相关距离分别达38754 m和56187 m,结构性因子是影响其空间变异的主要因子;土壤速效磷含量具有中等空间相关性,相关距离为24210 m,其空间变异受结构性因子和随机性因子共同影响。2) 土壤速效氮含量主要由北向南逐渐降低;速效磷含量主要在东北向西南及东南向西北方向上逐渐降低,而速效钾含量主要由东南向西北逐渐降低。3) 土壤速效氮含量在不同土壤类型及地形地貌间呈极显著差异;土壤速效磷含量在不同成土母质及地形地貌间呈显著或极显著差异;土壤速效钾含量在不同成土母质间呈极显著差异。土壤速效氮、磷、钾含量高值区单位面积化肥施用量明显高于低值区。     

Effects of elevated CO2 on the development and physiological metabolic activities of Nilaparvata lugens in response to the infection of Trichoderma asperellum

Trichoderma spp. are widely used in agriculture as biofungicides. In this study, effects of elevated CO₂ on the growth and enzyme activities of Nilaparvata lugens infected by T. asperellum were studied. The results showed that fecundity of N. lugens adversely decreased under elevated CO₂ relative to ambient CO₂ irrespective of infection with T. asperellum. The contents of protein and alkaline phosphatase significantly decreased in N. lugens nymphs with T. asperellum infection under elevated CO₂ relative to ambient CO_2. Significantly higher activities of carboxylesterase and acetylcholinesterase were observed in N. lugens infested with T. asperellum under elevated CO₂ compared with ambient CO₂. While significantly lower catalase and glutathione S-transferase were observed in N. lugens nymphs infested by T. asperellum under elevated CO₂ relative to ambient CO₂. Measuring the antioxidases and detoxification enzymes in N. lugens infected with T. asperellum can partly explain the response of fitness parameters of N. lugens under elevated CO₂.     

A molecular diagnosis method using real-time PCR for quantification and detection of Fusarium oxysporum f. sp. cubense race 4

The Fusarium genus causes devastating plant diseases worldwide, in which Fusarium oxysporum is the most serious crop pathogen. Disease monitoring is the basis of integrated pest management of any disease. The lack of rapid, accurate, and reliable device to detect and identify plant pathogens is one of the main limitations in integrated disease management. This study describes an efficient and quantifiable diagnosis method for the specific detection of F. oxysporum f. sp. cubense (Foc) race 4 in field-infected banana. With the optimized PCR parameters using the SCAR (sequence characterized amplified region) primers FocSc-1/FocSc-2 and a real-time PCR strategy, the developed method showed high reproducibility and was very sensitive to detect extremely low quantities of Foc genomic DNA (gDNA). We also found that Foc gDNA in severely symptomatic banana pseudostems and leaves were 6946-fold and 26.69-fold more than in those of mild-symptomatic banana, respectively.     

Molecular characterization and phylogenetic analysis of Citrus viroid V isolates from Pakistan

Citrus viroid V (CVd-V) was recently characterized and belongs to the genus Apscaviroid within the family Pospiviroidae. 334 CVd-V isolates were identified from Punjab, Pakistan, where CVd-V had not been reported. A total of 68 independent cDNA clones were sequenced from 11 infected trees of different cultivars, ranging from 292 to 295 nucleotides. The nucleotide diversity estimated from the nucleotide distances of the CVd-V Pakistan population was similar to that reported from other countries. Based on genetic diversity and phylogenetic analysis, two main CVd-V groups were identified indicating that Pakistan might be one of the geographic origins of CVd-V worldwide. We demonstrated that this viroid has not emerged recently and it is more widespread than previously expected.     

基于光能利用率模型估算大兴安岭北部火烧迹地生产力

以大兴安岭火后不同强度(轻度、中度、重度)及未火烧区森林为研究对象,利用光能利用率模型和ETM+影像,结合植被资料,估算了火烧结束12 a后(1999年8月)过火区的针叶林、阔叶林、针阔混交林的净初级生产力(Net primary productivity,NP,P),分析了1987年"5.6"大火不同强度火烧对森林分布和生产力的影响。结果表明:轻度和中度过火区林地面积的比重与未过火区相近,且NP,P比未过火区高;重度过火区部分林地的比重降低,森林退化成非林地,但林地NP,P与未过火区相近。     

施肥对麻疯树幼树生长与叶片养分动态的影响

[目的]探讨麻疯树成熟叶片N、P2O5、K2O、Ca、Mg、Fe、Mn、Cu、Zn、B共10种营养元素含量的动态变化和相关性,为麻疯树科学施肥提供参考依据.[方法]选取长势一致的麻疯树进行每株0、0.2、0.4、0.6kg的复合肥施肥处理,在每月的中下旬取植株成熟叶片,测定其营养元素的动态变化特征,分析其相互关系.[结果]麻疯树叶片营养元素含量高低顺序为：K2O＞N＞Ca＞ Mg＞P2O5＞Mn＞Fe＞Zn＞B＞Cu.不同施肥处理对叶片营养元素含量有一定影响,其中N、K2O、Ca、Mn、Fe、Zn、B元素含量受施肥影响比较大,而P2O5、Mg、Cu受影响比较小.麻疯树的株高、总新梢生长量、地茎粗随着施肥量的增加而增加,果枝率随着施肥量的增加先增加后降低,果枝率最高的是施肥0.4 kg/株处理.[结论]施肥能促进麻疯树的生长,0.4 kg/株/年（15-15-15复合肥）时最利于麻疯树果枝的形成.麻疯树叶片的营养元素受施肥的影响,养分元素间有协同和拮抗作用,生产上要根据麻疯树的营养需求规律和需肥特性进行平衡施肥.     

拉美解决粮食问题的典范——以委内瑞拉粮食主权运动为考察对象

粮食主权作为一种宗旨,正成为发展中国家解决粮食问题的指导思想,委内瑞拉查维斯政府在土地改革基础上推行的粮食主权运动是最早实践粮食主权宗旨的国家之一。通过对委内瑞拉粮食主权运动推行的背景、过程、措施的分析阐述,认为拉美各国只要在政府层面制定一个清晰的规划和农业发展战略,能够适应粮食市场的发展规律,提高小农的生产力水平,同时能最大限度地减少发达国家廉价粮食的冲击就可以真正解决粮食匮乏所带来社会、经济等问题。     

厌氧发酵生物技术处理果蔬废弃物分析及展望——基于北京新发地农产品批发市场的调查

新发地作为北京市交易规模最大的农产品专业批发市场,其产生的垃圾（主要为果蔬垃圾）日益增加,已经严重影响附近居民的生活。通过对新发地市场实地调查,分析该地垃圾（主要为果蔬垃圾）的产生数量、成分和目前的处理方式,充分验证处理新发地垃圾的急迫性和重要性,以及厌氧发酵处理的可行性和优越性;此外,对新发地垃圾进行批式厌氧发酵产气试验,在添加底物为3gVS／L、污泥负荷（F／M）为0．5下,经过35d的发酵反应,累计产气量达701mL／gVs,挥发性固体去除率为80％,实验表明新发地垃圾产气潜力大,为实际解决新发地垃圾问题提供了理论保障．     

农户参与农地股份合作制意愿的影响因素研究——基于湖北省三汊镇的农户调查

农地股份合作制作为一种新的土地流转方式,在实践过程中极大地显示了其制度的优越性。通过对湖北省三汊镇农户的入户调查,采用Logistic模型,实证分析了影响农户土地入股意愿的内部因素,并加以规范分析找出了影响农户入股意愿的主要外部因素。研究结果表明,农户入股意愿主要受户主的职业、家庭主要收入来源和家庭人口数量等内部因素和劳保福利、完善的社保等外部因素的影响,并据此提出了相应的促进农地股份合作制的建议。最后,对其前景进行了展望。