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31.
To elucidate the structure of the network of the water retention mechanism of fish-meat gel, the microscopic relationship between the water content and viscoelasticity was investigated, and the specific surface area (S) where the water molecules are adsorbed was also measured. The gel structures of fish-meat, actomyosin (AM), and myosin (M) were investigated. In the fish-meat gel, as the water content increased, the number of network chains (??) decreased and the molecular weight between the cross-linking points (M e) increased. At 10?% salinity and approximately 79?% moisture, ?? decreased to a minimum and M e began to increase remarkably. In addition, when the moisture increased to 78?%, S increased about 1.5 times and the elasticity decreased significantly, indicating that this level of water content is the limit for sufficiently forming a gel structure in fish-meat gel. In the AM and M gels, M e and S were similar to those observed in the fish-meat gels. When the moisture content increased, they also increased, regardless of the salt concentration. This result suggests that the size of the network was enlarged or that the network chains were cleaved. 相似文献
32.
Takai S Son WG Lee DS Madarame H Seki I Yamatoda N Kimura A Kakuda T Sasaki Y Tsubaki S Lim YK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(12):1313-1317
Rhodococcus equi was isolated from fecal and soil samples from four native Jeju horse farms and six Thoroughbred farms in Jeju, Korea. The isolates were examined for the presence of virulence-associated 15-17-kDa antigens (VapA) by colony blotting, using the monoclonal antibody 10G5, and for the gene encoding VapA by PCR. R. equi was isolated from all 36 soil samples collected from the 10 farms with between 5.0 x 10(2) and 7.5 x 10(4) colony-forming units (cfu) per gram of soil, and from 37 of 40 fecal samples with between 5.0 x 10(1) and 1.1 x 10 (5) cfu per gram of feces. Virulent R. equi was isolated from seven farms and appeared in 2.0% of isolates (10 of 508). Of the 10 virulent isolates, four contained a 90-kb type II plasmid, which has been found in isolates from the Kiso native horses of Japan, and the other six contained a new variant, which did not display the EcoRI and EcoT22I digestion patterns of the 10 representative plasmids already reported (85-kb types I, II, III, and IV; 87-kb types I and II; 90-kb types I, II, III, and IV). We designated the new variant as the "90-kb type V" plasmid, because its EcoRI digestion pattern is similar to that of the 90-kb type II plasmid. This is the first report of the prevalence of virulent R. equi in Jeju, Korea. The same virulence plasmid type is found in both Korean and Japanese isolates, providing insight into the origin, ancestry, and dispersal of native horses in Korea and Japan. 相似文献
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Takuma Aoki Hiroo Madarame Keisuke Sugimoto Hiroshi Sunahara Yoko Fujii Eiichi Kanai Tetsuro Ito 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):745-748
We report the case of a 4-year-old, castrated 4.2-kg Scottish fold cat with recurrent epistaxis that was unresponsive to medical therapy. Diathermocoagulation of the nasal mucosa with a diode laser controlled the epistaxis and there was no significant recurrence of epistaxis during 1 year of follow-up. 相似文献
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丁壮 Shinji TAKAI Hiroo MADARAME 常爽 黄海楠 霍晓伟 高明华 谭忠田 高双成 Fumiko HATORI Yukako SASAKI Tsutomu KAKUDA Shiro TSUBAKI 《中国兽医学报》2008,28(1):40-44
对中国内蒙古自治区饲育马土壤环境中马红球菌的分布知之甚少;为此,在中国内蒙古通辽近郊、内蒙古南部锡林郭勒盟大草原、内蒙古东部呼伦贝尔大草原等马场共收集了108份土壤样品进行了马红球菌的检测。结果,锡林郭勒盟大草原和呼伦贝尔大草原的马红球菌分离率为25.9%~30.0%,通辽近郊土壤中分离率高达82.3%。这说明在通辽近郊马红球菌的分离菌数是草原土壤中马红球菌分离菌数的10倍。应用PCR技术检测了488株的毒力相关基因,相对分子质量分别为15000~17000(VapA)和20000(VapB)。所有的分离株都未检出毒力相关基因。这些无毒力分离株的质粒资料显示,各种大小的潜在质粒的发生率为13.3%~21.5%。本研究结果截然不同于我们新近对蒙古国的调查结论:马红球菌在蒙古国乌兰巴托的马群中不存在。造成这一差异的原因可能在于蒙古国的游牧生活和内蒙古的非游牧生活。 相似文献
35.
Results of the preparation and characterization of homopolymers of 2-methacryloyloxyethyl isocyanate (MOI) and co-polymers of MOI with styrene synthesized by radical polymerization are shown. The polymers were significantly effective as additives for paper dry and wet strength. Dry strength can be increased by 75% and wet strength retention of sheets can reach 30% based on the increased dry strength and about 50% based on the dry strength of untreated sheet. In terms of sheet wet strength improvement, based on isocyanate group consumption, copolymers containing less isocyanate obtain a better effect than homopolymers. Furthermore, base paper containing a small amount of polyallylamine hydrochloride was used for polymer treatment and produced more wet strength improvement. 相似文献
36.
Kawarai S Hashizaki K Kitao S Nagano S Madarame H Neo S Ishikawa T Furuichi M Hisasue M Tsuchiya R Tsujimoto H Yamada T 《Veterinary immunology and immunopathology》2006,113(1-2):30-36
A primary cultured cell line named CHKS was established from a hepatocellular carcinoma (HCC) of a dog showing a high level of serum alpha-fetoprotein (AFP). CHKS secreted a 66 KDD AFP into the growth medium regardless of the presence or absence of fetal bovine serum (FBS). Cloning CHKS with limiting dilution produced 4 clones, CHKS-1, -2, -3, and -4, which secreted 826, 471, 70, and less than 10 ng/ml, respectively, of AFP into the culture medium. In culture, these cell lines were similar in morphology and proliferation pattern to epithelial cells and positive to periodic acid-Schiff (PAS) staining. The presence of mRNA for canine albumin was demonstrated by nested PCR. The doubling times of the clone cell lines were 21, 45, 36, and 35 h, saturation densities 34, 18, 22, and 24 x 10(4)/cm(2), and plating efficiencies 18, 45, 46, and 45%, respectively. Chromosome analysis of these cell lines showed near triploidy. These results show that CHKS and its clones have hepatic cell functions and are useful for carcinogenetic and clinical studies of canine HCC. 相似文献
37.
Rupture process of the 2004 Sumatra-Andaman earthquake 总被引:5,自引:0,他引:5
Ammon CJ Ji C Thio HK Robinson D Ni S Hjorleifsdottir V Kanamori H Lay T Das S Helmberger D Ichinose G Polet J Wald D 《Science (New York, N.Y.)》2005,308(5725):1133-1139
The 26 December 2004 Sumatra-Andaman earthquake initiated slowly, with small slip and a slow rupture speed for the first 40 to 60 seconds. Then the rupture expanded at a speed of about 2.5 kilometers per second toward the north northwest, extending 1200 to 1300 kilometers along the Andaman trough. Peak displacements reached approximately 15 meters along a 600-kilometer segment of the plate boundary offshore of northwestern Sumatra and the southern Nicobar islands. Slip was less in the northern 400 to 500 kilometers of the aftershock zone, and at least some slip in that region may have occurred on a time scale beyond the seismic band. 相似文献
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39.
QUANTITATIVE ESTERIFICATION OF ROSIN BY REACTION OF SODIUM ROSINATE WITH ALKYL HALIDES 总被引:1,自引:0,他引:1
应用松香钠盐和卤代烃的反应在110℃于N-甲基吡咯酮中定量合成了各种松香酯。依卤代烃结构的差异,酯化时间在30~2400min之间 相似文献
40.
The potential for earthquake early warning in southern California 总被引:11,自引:0,他引:11
Earthquake mitigation efforts in the United States currently use long-term probabilistic hazard assessments and rapid post-earthquake notification to reduce the potential damage of earthquakes. Here we present the seismological design for and demonstrate the feasibility of a short-term hazard warning system. Using data from past earthquakes, we show that our Earthquake Alarm System (ElarmS) could, with current TriNet instrumentation, issue a warning a few to tens of seconds ahead of damaging ground motion. The system uses the frequency content of the P-wave arrival to determine earthquake magnitude, an approach that allows magnitude determination before any damaging ground motion occurs. 相似文献