Rat urotensin-II-induced main pulmonary artery contractions are mediated by MAPK

AIM: To investigate rat Urotensin-II(rat U-II)-induced vasoconstriction of rat main pulmonary arteries and the role of mitogen-activated protein kinase(MAPK). METHODS: The main pulmonary artery was dissected from the male Sprague-Dawley rats and artery ring width was 3-4 mm. Concentration-response curves were generated to rat U-II(0.03 nmol/L-30 nmol/L).Inhibitor of MAPK, PD 98059(0.1 μmol/L-10 μmol/L) were added into the medium after rat U-II(30 nmol/L)induced vasoconstriction had reached plateau to construct the relaxant concentration-response curves and their EC₅₀ and E_max. RESULTS：Rat U-II was a potent vasoconstrictor of isolated rat main pulmonary arteries [EC₅₀=7.95±0.40, E_max=(14.28±6.34)% of the response to 60 mmol/L KCl]; PD 98059 caused concentration-dependent relaxations of rat U-II precontracted arteries [EC₅₀=5.91±0.45, E_max=(81.39±13.65)%]. CONCLUSION: Rat U-II was a potent vasoconstrictor of rat main pulmonary arteries and this response was mediated through MAPK.     

营养和环境条件对杏鲍菇菌丝生长的影响

研究了杏鲍菇菌丝生长的营养需求和环境条件，结果表明，适宜杏鲍菇菌丝生长的最佳碳源是葡萄糖、最佳氮源是黄豆粉，最佳碳氮比是60：1，最佳无机盐是硫酸钙。杏鲍菇菌丝生长的适宜温度为25℃，培养料最适含量为75%，最适pH为5.4。     

猴头菌优良菌株筛选研究初报

通过对六个猴头菌菌株菌丝生长情况，子实体农艺性状和产量的比较试验发现，HN、RT和猴杰2号综合性状较优，适合新疆阿拉尔地区代料栽培。     

Effect of protein kinase C on the expression of myocardial HSP70 mRNA during myocardic ischemic preconditioning in rabbits

AIM:To study the relationship between myocardial HSP70 and PKC during myocardial ischemic preconditioning(IPC). METHODS: PKC inhibitor, polymyxin B(PMB) and PKC activator, phorbol 12-myristate 13-acetate(PMA) were applied to the models of myocardial ischemia/reperfusion in vivo and in vitro in rabbits, respectively, and the ventricular functions, PLA₂ in the serum, and the expression of mycardial HSP70 mRNA were examined. RESULTS:IPC decreased PLA₂ activity, improved the left ventricular function and increased the expression of myocardial HSP70 mRNA. Howerer, all these effects of IPC could be blocked by PMB. Interestingly, PMA mimicked IPC with attenuating the injuries of cardial myocytes and increasing myocardial HSP70 mRNA expression. CONCLUSION:PKC is involved in the myocardial HSP70 expression in case of ischemic preconditioning.     

几种菌根型食用菌的菌根合成

本文简要介绍 6种共生型菌根食 (药 )用菌在相应树苗上完成菌根合成 ,并成功地长出子实体的事例 ,说明菌根食用菌的发展途径 ;就有关菌根合成的方法及技术作一初步介绍 ;对菌根合成技术中应注意的关键问题进行探讨 ;对菌根型食用菌今后的研究与发展道路提出了建议。     

巴楚蘑菇孢子弹射与收集方法的研究

巴楚蘑菇在新疆塔里木盆地极其炎热干燥气候条件下，其孢子与一般子囊菌的一样可向空气中弹射，但主要是以耳片在水中弹射孢子的方式进行孢子释放，孢子随水进行传播。孢子收集时将耳片放入水中诱导耳片释放孢子，可比较容易地收集到无菌孢子。     

结核分枝杆菌分泌蛋白ESAT-6、CFP10基因的克隆、鉴定及其原核表达

以人型结核分枝杆菌 H37RV株基因组 DNA为模板 ,应用 PCR法对 ESAT- 6和 CFP10基因进行扩增 ,产物经纯化后与载体 PMD18- T连接、转化及酶切鉴定 ,亚克隆到原核表达载体 PGEX- 6 P- 1,构建原核重组表达质粒 ,转化入大肠杆菌 BL2 1中 ,以 1mmol/L IPTG诱导 ,进行 SDS- PAGE电泳。结果表明 ,ESAT- 6和 CFP10基因表达的融合蛋白相对分子质量分别为 32 0 0 0和 36 0 0 0 ,与实测相符。重组结核杆菌分泌蛋白 ESAT- 6和 CFP10的成功表达为结核病诊断及重组疫苗的构建打下了基础     

大肠杆菌新型菌毛(F1987)的基因定位

对动物产肠毒素性大肠杆菌 (ETEC)的一种新型菌毛 (F1987)进行了相应基因的定位研究 ,通过对表达该新型菌毛 (F1987)相应 ETEC菌株的培养、质粒提取、对大肠杆菌受体菌株 DH5α的转化及阳性转化子筛选与相应菌毛表达的检定等 ,初步表明该新型菌毛 (F1987)的基因定位于质粒上     

昆虫抗菌肽生物学活性及其应用前景

昆虫抗菌肽是昆虫免疫后存在于血淋巴中的一类活性肽 ,具有广谱的抗菌、抗病毒、抑制肿瘤的生物活性 ,具有很高的应用潜力。本文主要介绍昆虫抗菌肽的类型、生理活性、基因的克隆与表达及在动物科学中的应用前景     

猪胸膜肺炎放线杆菌外膜蛋白(OMP)5′末端保守区基因的克隆、表达及其免疫活性研究

以猪胸膜肺炎放线杆菌血清7型25-4株基因组DNA为模板,PCR方法扩增外膜蛋白(OMP)5′末端保守区基因片段(OMPc),酶切及核苷酸序列分析鉴定后,与原核表达载体质粒pGEX-6P-1进行连接,构建成重组表达载体pGEX-OM-Pc,转入大肠杆菌BL21中,以IPTG进行诱导,SDS-PAGE电泳分析发现,转化了重组质粒的菌株所表达的融合蛋白相对分子量为34 kD,与实际预测相符,命名为GST-OMPc.GST亲和层析柱进行纯化,ELISA方法对纯化蛋白进行检测.结果表明:纯化蛋白GST-OMPc能够与兔抗猪胸膜肺炎放线杆菌血清7型的阳性血清反应.OMPc蛋白的成功表达为其功能的研究打下基础.