全文获取类型
收费全文 | 178篇 |
免费 | 10篇 |
专业分类
林业 | 25篇 |
农学 | 1篇 |
23篇 | |
综合类 | 11篇 |
农作物 | 7篇 |
水产渔业 | 9篇 |
畜牧兽医 | 104篇 |
园艺 | 5篇 |
植物保护 | 3篇 |
出版年
2021年 | 5篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 9篇 |
2016年 | 3篇 |
2015年 | 5篇 |
2014年 | 5篇 |
2013年 | 15篇 |
2012年 | 16篇 |
2011年 | 21篇 |
2010年 | 6篇 |
2009年 | 9篇 |
2008年 | 7篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 9篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1966年 | 2篇 |
1963年 | 1篇 |
排序方式: 共有188条查询结果,搜索用时 15 毫秒
101.
Robert J. Brosnan Fabíola B. Fukushima Trung L. Pham 《Veterinary anaesthesia and analgesia》2017,44(3):577-588
Objective
N-butane and n-pentane can both produce general anesthesia. Both compounds potentiate γ-aminobutyric acid type A (GABAA) receptor function, but only butane inhibits N-methyl-d-aspartate (NMDA) receptors. It was hypothesized that butane and pentane would exhibit anesthetic synergy due to their different actions on ligand-gated ion channels.Study design
Prospective experimental study.Animals
A total of four Xenopus laevis frogs and 43 Sprague–Dawley rats.Methods
Alkane concentrations for all studies were determined via gas chromatography. Using a Xenopus oocyte expression model, standard two-electrode voltage clamp techniques were used to measure NMDA and GABAA receptor responses in vitro as a function of butane and pentane concentrations relevant to anesthesia. The minimum alveolar concentrations (MAC) of butane and pentane were measured separately in rats, and then pentane MAC was measured during coadministration of 0.25, 0.50 or 0.75 times MAC of butane. An isobole with 95% confidence intervals was constructed using regression analysis. A sum of butane and pentane that was statistically less than the lower-end confidence bound isobole indicated a synergistic interaction.Results
Both butane and pentane dose-dependently potentiated GABAA receptor currents over the study concentration range. Butane dose-dependently inhibited NMDA receptor currents, but pentane did not modulate NMDA receptors. Butane and pentane MAC in rats was 39.4 ± 0.7 and 13.7 ± 0.4 %, respectively. A small but significant (p < 0.03) synergistic anesthetic effect with pentane was observed during administration of either 0.50 or 0.75 × MAC butane.Conclusions
Butane and pentane show synergistic anesthetic effects in vivo consistent with their different in vitro receptor effects.Clinical relevance
Findings support the relevance of NMDA receptors in mediating anesthetic actions for some, but not all, inhaled agents. 相似文献102.
T. Tamamoto K. Ohno M. Takahashi K. Fukushima H. Kanemoto Y. Fujino H. Tsujimoto 《Veterinary and comparative oncology》2017,15(1):1-5
In this study, plasma MMP‐9 activity was evaluated in cats with lymphoma. Plasma samples were obtained from 26 cats with lymphoma before treatment. From 13 of the included 26 cats, plasma samples were obtained 4 weeks after the initiation of treatment. Plasma samples were also obtained from 10 healthy cats as a control. Plasma MMP‐9 activity was examined by gelatin zymography and semi‐quantitative value (arbitrary unit; a.u.) for each sample was calculated. Relatively high levels of MMP‐9 were observed in cats with lymphoma compared with those in healthy control cats. MMP‐9 quantification through zymography showed significantly higher activity in cats with lymphoma (median, 0.63 a.u.; range, 0.23–3.24 a.u.) than in healthy controls (0.22 a.u.; 0.12–0.46 a.u.; P < 0.01). MMP‐9 activities were significantly different before (0.73 a.u.; 0.30–3.24 a.u.) and after treatment (0.50 a.u.; 0.14–1.32 a.u.; P = 0.017). Measuring plasma MMP‐9 activity in cats with lymphoma may become an appropriate monitoring tool for feline lymphoma. 相似文献
103.
104.
Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China
Kariwa H Zhong CB Araki K Yoshimatsu K Lokugamage K Lokugamage N Murphy ME Mizutani T Arikawa J Fukushima H Xiong H Jiehua C Takashima I 《The Japanese journal of veterinary research》2001,49(2):105-114
Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China. 相似文献
105.
A competitive enzyme‐linked immunosorbent assay (ELISA) has been developed for the detection of the insecticide flucythrinate in environmental and food samples. Two types of haptens, the acid moiety that is the hydrolyzed product of flucythrinate, and the carboxylated propyl derivative of the alcohol moiety, were used to prepare monoclonal antibodies (MAbs). Five MAbs, which raised against the former hapten, were reactive with flucythrinate. Among them, MAb F1A27‐4 showed the highest activity toward flucythrinate, and did not cross‐react with other pyrethroids such as cycloprothrin, fenvalerate, fluvalinate, etofenprox and silafluofen. The assay conditions of indirect competitive ELISA with MAb F1A27‐4 were studied to optimize the detection of flucythrinate in environmental and food samples. Incubation at 4 °C in the assay buffer, pH 8, with 300 mM sodium chloride improved the sensitivity. The addition of rabbit serum albumin or rabbit antiserum and the presence of 50 ml litre?1 of methanol reduced matrix effects of the samples. Under optimized conditions, the ELISA detected flucythrinate spiked in water, soil, and extracts of apple and tea samples down to 10 mg litre?1, 0.2 mg litre?1, 0.3 mg litre?1 and 0.3 mg litre?1, respectively. The mean recovery and CV ranged from 91% to 120% and from 5% to 12%, respectively. The ELISA results in apple samples correlated well with those from LC–MS analysis (r2 = 0.99, n = 12). © 2001 Society of Chemical Industry 相似文献
106.
A decline in the hot-water-soluble pectin and an increase in the hot-water-insoluble pectin were observed when cucumber fruits were subjected to chilling temperatures. Infrared absorption spectra revealed the presence of highly esterified carboxyl groups in the soluble pectin, and of free carboxyl groups in the insoluble pectin. An increase of the insoluble pectin during chilling was also found in other chilling-sensitive plants. From these results it is suggested that a de-esterification of pectin and the concomitant increase of polymeric pectin takes place during chilling, making cell walls firmer, and that these pectic changes may be a characteristic common to a number of chilling-sensitive plants. 相似文献
107.
The lignin biosynthetic pathway in Eucalyptus camaldulensis was investigated by feeding stems with deuterium-labeled precursor. Pentadeutero[,-D2 OCD3] coniferyl alcohol was synthesized and supplied to shoots of E. camaldulensis, and incorporation of the labeled precursor into lignin was traced by gas chromatography-mass spectrometry. In addition to the direct incorporation of labeled precursor into the guaiacyl unit, a pentadeuterium-labeled syringyl unit was detected. This finding indicates that the -deuterium atoms in the hydroxymethyl group of labeled coniferyl alcohol remain intact during modification of the aromatic ring. The relative level of trideuterium-labeled syringyl monomer (the result of conversion via the cinnamic acid pathway) was negligible, suggesting that the pathway at the monolignol stage is used for conversion of exogenously supplied precursor. Our results provide conclusive evidence of a novel alternative pathway for generation of lignin subunits at the monolignol stage even in plants that do not accumulate coniferin in lignifying tissues. 相似文献
108.
Masato Yoshida Daiji Fujiwara Yukiko Tsuji Kazuhiko Fukushima Teruko Nakamura Takashi Okuyama 《Journal of Wood Science》2005,51(5):448-454
To examine the effect of gravity on lignin content and deposition in plant cells, we used ultraviolet (UV) microspectrophotometry
and chemical methods to investigate the secondary xylem of Prunus jamasakura grown on a three-dimensional (3D) clinostat, which simulates microgravity. The stem of the 3D-clinostat specimens elongated
with bending and the width of their secondary phloem increased. The UV absorbance of the 3D-clinostat specimens at 278 nm
was higher than that of the control specimens, which were grown on the ground, in the wood fiber cell corner middle lamella,
compound middle lamella, and fiber secondary wall; the UV absorbance in the vessel secondary wall did not differ between the
specimens. The lignin content in the stem, including the bark, of the 3D-clinostat specimens, as determined using an acetyl
bromide method, was less than that of the control specimens. In the specimens that differentiated on a 3D clinostat, the amount
of lignin in the wood fibers increased, while the proportion of the lignified xylem in the stem decreased relative to control
values. 相似文献
109.
Effect of long-term administration of a prostacyclin analogue (beraprost sodium) on myocardial fibrosis in Dahl rats 总被引:1,自引:0,他引:1
Kaneshige T Saida Y Tanaka R Soda A Fukushima A Ida N Takenaka M Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(12):1271-1276
Beraprost sodium (BPS) is an orally active prostacyclin analogue. The effects of BPS on the heart, including coronary circulation improvement, myocardial and vascular protection and anti-fibrosis effect on myocardium interstitium, have previously been demonstrated. However, the effects of BPS on hemodynamics, cardiac function and myocardial contractility in patients in the hypertrophic phase have not been clarified. Therefore, in the present study, the effects of BPS under long-term administration were investigated using the hypertension model of salt-sensitive Dahl rats. Six-week-old Dahl rats were divided into three groups, an 8% high salt diet group treated with BPS (BPS group), an untreated 8% high salt diet group (HHF group) and an untreated 0.3% low salt diet group (Control group), and observations were conducted until 17 weeks of age. In the BPS and HHF groups, the survival rates after 11 weeks of high salt diet intake were 87.5% and 47.1%, respectively (p<0.05). At 17 weeks of age, the atrial systolic peak velocity/early diastolic peak velocity and heart weight index of the BPS group decreased significantly compared with the HHF group (p<0.05). The HHF group exhibited significantly more severe myocardial fibrosis mainly in the endocardial layer of the left and right ventricles compared with the BPS and Control groups (p<0.05). In the present study, long-term BPS administration preserved diastolic function and prevented myocardial interstitial fibrosis in the non-compensatory phase. The results of the present study suggest that BPS is effective for treatment of hypertensive cardiac hypertrophy. 相似文献
110.
Artificial insemination (AI) subfertility is an indication of failure of AI with frozen-thawed sperm classified as normal by conventional semen examination. Recently, 8 AI-subfertile Japanese Black cattle (S1-S8) were identified using the routine AI test or in vivo fertilization test, which included AI with frozen-thawed sperm of superovulated females and subsequent non-surgical recovery of presumptive zygotes. In the present study, we assessed capacitation states and in vitro oocyte penetration of frozen-thawed sperm from these bulls to estimate causal factors of AI subfertility. Frozen-thawed sperm from 8 AI-subfertile (S1-S8) and 9 fertile (F1-F9, control) bulls were washed and then used for a chlortetracycline (CTC) staining assay and in vitro fertilization test. The CTC staining assay revealed that approximately 50% of the sperm from 4 of the AI-subfertile bulls (S5-S8) were prematurely progressing into the capacitation state immediately after washing and resuspension in a CaCl(2)-lacking medium. In contrast, most of the sperm from the fertile bulls and other AI-subfertile bulls (S1-S4) remained uncapacitated. Addition of CaCl(2) to the medium effectively promoted a spontaneous acrosome reaction in the sperm samples from the AI-subfertile bulls (S5-S8). Moreover, the in vitro fertilization test showed that rates of sperm penetration into oocytes were significantly lower in sperm samples from the AI-subfertile bulls (S5-S8) than in the control sperm samples from the fertile bulls (F2-F4 and F7-F9). It has previously been suggested that prematurely capacitated sperm undergo a spontaneous acrosome reaction possibly due to uncontrolled influx of calcium ion, and consequently they possess relatively lower in vitro fertilizing ability. It is therefore possible that premature capacitation of sperm used for AI is a causal factor of subfertility of male Japanese Black cattle and a potentially good marker for identification of subfertile bulls for removal from AI programs. 相似文献