应用多重PCR检测人工感染鸡呼吸道疾病的研究

本文报道了IBV，NDV，ILTV，MG人工感染4周龄SPF鸡和非免疫鸡后，用多重PCR检测试验鸡的咽喉棉拭子和器官组织样品，并与传统的病原分离鉴定，血清学方法进行比较，多重PCR无论是对单一感染病原，还是对两种以上混合感染病原，其敏感性和检测速度都优于传统的鉴别诊断方法，具有较高的实用价值，可以直接应用于临床检测，服务于生产。     

Reproduction in three species of rainbowfish (Melanotaeniidae) from rainforest streams in northern Queensland, Australia

Abstract – The reproductive biology of three species of rainbowfish (Melanotaeniidae) in northeastern Australian rainforest streams was investigated. Two species, Melanotaenia eachamensis and Cairnsichthys rhombosomoides are endemic to the area, whereas the third, M. splendida splendida, is more widespread. The species were all highly fecund, producing many hundreds of eggs between 1.10 and 1.24 mm in diameter. Melanotaenia eachamensis was the most fecund, produced the largest eggs of the three species, and consequently exhibited the greatest maternal investment (as measured by gonadosomatic index). The majority of reproductive effort occurred during the dry season, although reproductively active fish were present year-round for each of the species, but particularly so for M. s. splendida and C. rhombosomoides. No evidence for a role by temperature or photoperiod as environmental cues for reproduction was found, and it was suggested that gonad development was strongly tied to somatic growth. The concentration of reproduction to the dry season ensures that larvae are produced during a period of relatively stable and benign physical conditions. Comparison of temporal changes in gonadosomatic index values suggest that the spawning season of M. eachamensis , which occurs in high-elevation streams, is more restricted and commences about 1 month earlier than either other species. A similar phenology was observed for the M. s. splendida population found at high elevation and highlights the potential for spatial differences in stream productivity to influence life history. ^Note     

Validation of the otoliths for age determination in Barbus sclateri (Günth.)

Abstract – Otoliths were used to explore the timing of annulus formation in Barbus sclateri. Lapillus exhibited opaque and translucent zones whose combination was formed once a year. Opaque zones appeared formed from July and January, and the translucent ones from February to June. We describe a procedure to assess age by using the number of annulii, the condition of the otolith's edge and the capture date. The use of otoliths for age determination in this cyprinid endemic to the Iberian Peninsula provides a useful alternative to the traditional methods of scale reading. ^Note     

Ionic Ratios and Crop Performance: II. Effects of Interactions amongst Vanadium, Phosphorus, Magnesium and Calcium on Soybean Yield

Because vanadium (V) is easily reduced to a cationic form within plant cells, data from resin-extraction of soil were analysed for evidence of interactions between V and the resin-extractable concentrations of magnesium (Mg) and calcium (Ca) on soybean seed yield. Three varieties, 9091, 9061 and 704, were grown over a 3-year period in a corn–soybean–wheat rotation. Surface soil samples (0–15 cm) were extracted with ion-exchange resins, extracts were analysed by inductively coupled plasma methods (ICP), and the results were regressed against seed yield using SAS PROC STEPWISE analysis using forward selection, backward elimination and maximum R² routines. The seed yield of each variety showed a correlation with a unique set of resin-extractable concentrations of V, phosphorus (P), Mg and Ca, and the V:(V + P), Mg:(Mg + Ca), Mg:(Mg + 1000 V) and Ca:(Ca + 1000 V) ratios. Variety 9091 was most sensitive to the Mg:(Mg + Ca) ratio. Variety 9061 was most sensitive to extractable V and to the V:(V + P) ratio. Variety 704 was sensitive to extractable P, V and Ca and the Mg:(Mg + 1000 V) ratio. For variety 9091, Mg fertilization (not currently practised) may be an economical practice, whereas P fertilization of 704 may not be economical. Each regression technique varied slightly in identification of important factors in seed yield. Concentrations and ratios of resin-extractable elements in soil provide insights into optimal genotype selection and possible management alternatives for a given soil.     

Social, economic and cultural perspectives of European inland fisheries: review of the EIFAC symposium on fisheries and society

The contributions presented at the EIFAC Symposium on Fisheries and Society in 2000 are reviewed. There have been considerable advances in many sectors of inland fisheries over the past decade. Nowadays the value of inland fisheries to society is generally better understood and increasingly recognized. However, it was recognized that the allocation of fishery resources and their management could still be made more effective through more extensive application of the recent approaches and techniques discussed during the Symposium and reviewed in the paper.     

Evaluation of two N cycle models for the prediction of N mineralization from grassland soils in the UK

Abstract. The ability of two nitrogen cycle models, of contrasting complexity, to predict N mineralization from a range of grassland soils in the UK, was evaluated. These were NCYCLE, a simple mass balance model of the N cycle in UK grasslands, and CENTURY, a more complex model simulating long-term C, N, P & S dynamics in grassland ecosystems. The models were tested using field measurements of net N mineralization from a range of grassland soils (differing in soil type, history & management practice), obtained over a 2 year period using a soil core incubation technique. This method was considered to measure the total net release of mineral N from the soil organic matter over a specified time, including N which may have been recycled several times. NCYCLE consistently under-estimated mineralization rates at all sites. By contrast, there was some correlation between CENTURY predictions of net N mineralization and field measurements. This may have reflected the different abilities of the two models to simulate N recycling. Neither model, however, was able to predict adequately the effect of cultivation and reseeding on net N mineralization.     

Factors Affecting the use of Dreissena polymorpha as a Bioindicator: the PCB Pollution in Lake Como (N. Italy)

The aim of this study was to assess the PCB pollutionof the South-Western branch of Lake Como by using thezebra mussel (Dreissena polymorpha) as abioindicator since its recent reintroduction. Someenvironmental and physiological variables influencingaccumulation processes of organochlorine compounds inDreissena were investigated because climaticfactors may affect life cycle, reproductive stage andage. PCB levels were highest in the soft tissues ofDreissena close to the industrialized area ofComo (2298 ng g^-1 of lipids) and slowly decrease goingnorth, reaching approximately half this value at theclosing section of the lake branch. Nonetheless, thePCB concentrations are well below the dangerous levelsfor the aquatic environment.     

Sinnesphysiologische Untersuchungen an Schmetterlingsraupen und ihre praktische Bedeutung

Ohne Zusammenfassung     

Puncture Wounds of the Navicular Bursa in 38 Horses A Retrospective Study

The medical records of 38 horses with puncture wounds of the navicular bursa were reviewed. Only 12 horses had a satisfactory outcome (breeding or riding). Of the remaining 26 horses, 19 were euthanized, five were sold due to persistent severe lameness, one died, and one was lost to long-term follow-up. Different combinations of conservative management prior to surgical debridement and drainage of the navicular bursa were unsuccessful in resolving the condition. Horses that were treated surgically within 1 week of the injury and had a hind leg affected had the best chance of a satisfactory outcome. Additional wound debridement was necessary in 15 horses after initial surgical treatment. The most common complications encountered were navicular bone osteomyelitis and sepsis of the deep digital flexor tendon. Thirteen of 14 horses that had rupture of the deep digital flexor tendon and subluxation of the distal interphalangeal joint had an unsatisfactory outcome. One mare subsequently developed ankylosis of the distal interphalangeal joint and was a useful brood mare. Two horses that had biaxial palmar digital neurectomy because of persistent lameness were later euthanized because of navicular bone fracture and rupture of the deep digital flexor tendon. Results from limited numbers of bacterial cultures and antibiotic sensitivities suggest that penicillin and an aminoglycoside antibiotic should be used as initial antibiotic therapy. Immediate surgical debridement and appropriate antibiotic treatment are recommended as the minimum therapy for penetrating wounds of the navicular bursa in horses.