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乙型脑炎病毒NS1基因重组伪狂犬病毒的构建 总被引:7,自引:0,他引:7
设计1对引物从含有乙型脑炎病毒NS1基因的质粒pNS1上亚克隆NS1基因,将NS1基因插入到中间转移载体pUSK中,获得重组中间转移质粒pUSK—NS1。将pUSK—NS1与伪狂犬病毒Ea株TK/gG/LacZ^ 突变株基因组共转染真核细胞IBRS-2,通过空斑纯化得到了乙型脑炎病毒NS1基因重组伪狂犬病毒株TK/gG^-/NS^ 1。经检测,重组病毒能表达具有生物活性的NS1蛋白。该重组病毒可作为猪乙型脑炎和伪狂犬病双价基因工程疫苗用毒株。 相似文献
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甜菜夜蛾(Spodopteraexigua Hübner)雄蛾触角对雌蛾性信息素的EAG反应 总被引:3,自引:0,他引:3
采用EAG生测技术研究表明,甜菜夜蛾雄蛾触角上具有4种性信息素组分(Z9,E12-14Ac(A)、Z9-14OH(B)、Z9-14Ac(C)和Z9,E12-14OH(D))的感受细胞.单一组分A,二元组分AB和AD,三元组分ABC、ABD和ACD及四元组分ABCD的EAG反应显著较高,与3头雌蛾性信息素腺体浸提液的反应强度相当.进一步对AB二元混合物中两组分的最佳比例以及最佳比例的剂量反应进行了测定.当AB间的比例为55时可产生最大的EAG反应,其它比例(91、73、37、19)间无明显差异.AB(55)混合物的使用剂量在0,04~4μg范围内,EAG值随剂量增加呈直线上升;使用剂量低于0.04μg或高于40μg,EAG值不再有明显变化. 相似文献
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An algorithm of fault section diagnosis based on topology identification for distribution networks is presented. By decomposing the topologic matrix which describing the distribution network into two parts,one part only contain the complex coupling factor ,and the other ignore the complex coupling factor. Using this method, the fault zones in distribution network can be identified and isolated efficiently, and the vertexes of the zones can also be identified automatically. This approach adapts to the changefully network structures. 相似文献
26.
Investment on construction projects has become an important factor in improving the development of economy in China.The non-price bottom bidding is a representation that the investment on projects is becoming standardized.During the years of trying out the non-price bottom bidding,the colluding in bidding or bidding below the cost has happened several times,which have damaged the benefits of owners.To solve theses problems,this paper firstly studies the reason of these problems-the failure in the normal relationship between principal and agent.Then,some measures have been presented as follows: building systematic and scientific system of qualification prejudication,judging behaviors of colluding in bidding and bidding below the cost with reason and providing a surety mechanism of the project.The authors hope that these measures are beneficial to non-price bottom bidding. 相似文献
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Novel surface treatment technique by silane film is developing for metallic anti corrosion and coat improvement. The key points, hydrolysis and condensation, for the stability of the Silane agent(SA) solution is investigated, and the effective factors of stability for silane agent solution and the relative rule are discussed. It is found that stability of SA solution is related to the temperature, pH, solvents, SA concentration and additive by studying the state of the solution and the conductivity-time curve of SA solution. Meanwhile, controlling factors and improving methods for SA stability are proposed to guide the practical application of metallic surface anti-corrosion treatment by SA. 相似文献
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Jinshajiang River, which has a drainage area of 485,100 Km 2, average flux of 142.9 billion m 3, and soil conveyance of 247 million t/a, is the largest branch of Changjiang river. It is also the largest soil resource of the Three Gorges reservoir and upper reach of Changjiang River. To research the development and trend of Jinshajiang sediment not only has a relation to Jinshajiang water resource strategy, but also has important meaning to control the silt in Three Gorges reservoir and the Changjiang water resource development. The paper statistically analyses the flux and sediment conveyance of the Pingshan station, which is the primary hydrological station in the Jinshajiang River Drainage Area. Through several non-parameter statistical methods such as Spearman order correlativity check, Kendell order correlativity check, and linear regression check, the paper concludes that the sediment evolvement trend of Pingshan station is not distinct. 相似文献
30.
In the civil industry, quality is a key factor, so quality management and quality control play decisive roles in construction especially. At present, the main method of construction is quality controlling that engineers and technicians consult the standard and norm concerned, then check them one by one, and supervise the construction. It is not only inefficient but also easy to make mistake. Using computer technology in the quality control of construction will promote quality control level and improve management measures. Through the research of the quality control of construction,the system of quality control in construction is studied and developed, the structure of the software and its developed technology are expounded. 相似文献