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排序方式: 共有163条查询结果,搜索用时 781 毫秒
111.
Nakano Y Matsuo S Tani H Sasai K Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(4):373-374
An eardrop solution of beta-thujaplicin was examined for therapeutic effects on canine Malassezia-related otitis externa. Half to one ml of beta-thujaplicin solution of 100 microg/ml including DMSO 2% was injected everyday into both external ear canals of 31 cases for test-of-cure agreement. Fifteen score phases were established from the symptoms and cerumen smear biopsy findings, and score changes were recorded at least once a week. The means of the second and the third inspection day scores decreased significantly more than the previous value of each. In addition, the numbers of yeast-like organisms clearly decreased. These results suggest that beta-thujaplicin eardrops are effective for Malassezia-related otitis externa in dogs. 相似文献
112.
Yukie Tada Norio Yano Hiroshi Takahashi Katsuhiro Yuzawa Hiroshi Ando Yoshikazu Kubo Akemichi Nagasawa Akiko Inomata Akio Ogata Dai Nakae 《Journal of toxicologic pathology》2013,26(4):393-403
Information about potential risks of iron nanomaterials is still limited, while a wide variety of applications are expected. We recently reported acute phase responses of male and female Fischer 344 rats after a single intratracheal spray instillation of Fe3O4 nanoparticles (magnetite), clearly showing dose-dependent pulmonary inflammatory changes (Tada et al., J Toxicol Pathol 25, 233–239, 2012). The present study assessed long-term responses of male and female Fischer 344 rats to multiple administrations of magnetite. Ten-week-old male and female Fischer 344 rats (n=20/group) were exposed to a total of 13 quadweekly intermittent intratracheal spray instillations of magnetite during the experimental period of 52 weeks, at doses of 0, 0.2 (low), 1.0 (medium) and 5.0 (high-dose) mg/kg body weight per administration. Absolute and relative lung weights of the high-dose group were significantly higher than those of the control group. Macroscopically, slight enlargement and scattered black patches were recognized in the lungs and the lung-associated lymph nodes of the high-dose group. Histopathologically, infiltration of macrophages phagocytosing magnetite (all dose groups) and of chronic inflammatory cells (medium- and high-dose males and high-dose females), alveolar bronchiolization and granuloma (high-dose group) were observed. In addition, alveolar hyperplasias were observed in some rats of the high-dose group, and cytoplasmic overexpression of β-catenin protein was immunohistochemically found in such lesions. The present results clearly show that instilled magnetite causes chronic inflammatory responses in the lung. These responses occur in a dose-dependent manner without apparent differences among sexes 相似文献
113.
Furosemide loading test in a case of homozygous solute carrier family 12, member 1 (SLC12A1) mutation (g.62382825G>A,p.Pro372Leu) in Japanese Black cattle
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Kiyotoshi Hasegawa Shinji Sasaki Yoichi Sakamoto Akifumi Takano Megumi Takayama Tomoko Higashi Yoshikazu Sugimoto Yasuaki Yasuda 《Animal Science Journal》2017,88(10):1459-1464
Hydrallantois is the excessive accumulation of fluid in the allantoic cavity in a pregnant animal and is associated with fetal death. We recently identified a recessive missense mutation in the solute carrier family 12, member 1 (SLC 12A1 ) gene (g.62382825G>A, p.Pro372Leu) that is associated with hydrallantois in Japanese Black cattle. Unexpectedly, we found a case of the homozygous risk‐allele for SLC 12A1 in a calf, using a PCR ‐based direct DNA sequencing test. The homozygote was outwardly healthy up to 3 months of age and the mother did not exhibit any clinical symptoms of hydrallantois. In order to validate these observations, we performed confirmation tests for the genotype and a diuretic loading test using furosemide, which inhibits the transporter activity of the SLC 12A1 protein. The results showed that the calf was really homozygous for the risk‐allele. In the homozygous calf, administration of furosemide did not alter urinary Na+ or Cl? levels, in contrast to the heterozygote and wild‐type calves in which these were significantly increased. These results demonstrate that the SLC 12A1 (g.62382825G>A, p.Pro372Leu) is a hypomorphic or loss‐of‐function mutation and the hydrallantois with this mutation shows incomplete penetrance in Japanese Black cattle. 相似文献
114.
以京藏香草莓(Fragaria ananassa Duch.cv.Jingzangxiang)匍匐茎茎尖为试验材料,以LED白光为对照,研究了红光、蓝光、组合光(红∶蓝∶远红=1∶1∶1)不同LED光质对草莓茎尖组织培养的影响。结果表明,在茎尖诱导分化阶段,丛生芽分化时间快慢依次为红光组合光白光蓝光;诱导分化率高低依次为组合光白光红光蓝光;增殖系数大小依次为组合光白光红光蓝光;生根率高低依次为组合光白光蓝光红光,植株生长势强弱依次为组合光白光红光蓝光。综合而言,LED组合光质有利于草莓茎尖组织培养。 相似文献
115.
Nao KOYAMA Yoshikazu UENO Yusuke EGUCHI Katsuji UETAKE Toshio TANAKA 《Animal Science Journal》2012,83(7):562-570
This study investigated the effects of changes in daily management on behavior of a solitary female elephant in a zoo. The activity budget and space utilization of the subject and the management changes were recorded for 1 year after the conspecific male died. The observation days could be categorized into five clusters (C1–C5) by the characteristic behavioral pattern of each day. C1 had the highest percentage of resting of all clusters, and was observed after the loss of the conspecific and the beginning of use of the indoor exhibition room at night. C2, which had the highest percentage of stereotypy of any cluster, was observed after the beginning of habituation to the indoor exhibition room. Also, when the time schedule of management was changed irregularly, the subject frequently exhibited stereotypic pacing (C2, C4). The subject tended to rest when exhibiting lameness in the left hind limb (C3). In C5, activity reached a high level when she could utilize a familiar place under a stable management schedule. These results indicate that management changes affected the mental stability of an elephant in the early stage of social isolation. 相似文献
116.
Aya Goto Jiro Sonoda Yuki Seki Yoshikazu Taketa Etsuko Ohta Kyoko Nakano Akira Inomata Kazuhiro Hayakawa Toyohiko Aoki Kazuo Tsukidate Satoru Hosokawa 《Journal of toxicologic pathology》2012,25(3):229-232
Maxillary gingivae from male and female Crl:CD(SD) rats at 12, 16, 21, and 34 weeks of
age were examined histologically. The incidence of gingivitis was approximately 40%, with
no age or sex predilection, and was most frequent between the first and second molar.
Lesions were characterized by acute focal neutrophilic infiltration into the gingival
mucosa, occasionally with inflammatory exudate. In severe cases, inflammation extended to
the periodontal ligament with abscess formation, and adjacent alveolar bone
destruction/resorption. The most characteristic finding was the presence of hair shafts
associated with the lesion, which was observed in approximately 80% of the rats with
gingivitis. These findings suggest that molar gingivitis occurs in rats from an early age
and persists thereafter, and that the main cause of gingivitis in rats is hair penetration
into the gingiva. It would be prudent to keep these background lesions in mind as
potential modifiers in toxicity studies. 相似文献
117.
Yukie Tada Norio Yano Hiroshi Takahashi Katsuhiro Yuzawa Hiroshi Ando Yoshikazu Kubo Akemichi Nagasawa Akio Ogata Dai Nakae 《Journal of toxicologic pathology》2012,25(4):233-239
Iron nanomaterials are of considerable interest for application to
nanotechnology-related fields including environmental catalysis, biomedical imaging, drug
delivery and hyperthermia, because of their superparamagnetic characteristics and high
catalytic abilities. However, information about potential risks of iron nanomaterials is
limited. The present study assessed pulmonary responses to a single intratracheal spray
instillation of triiron tetraoxide nanoparticles (magnetite) in rats. Ten-week-old male
and female Fischer 344 rats (n=5/group) were exposed to a single intratracheal spray
instillation of 0 (vehicle), 5.0, 15.0 or 45.0 mg/kg body weight (BW) of magnetite. After
14 days, the rats were sacrificed, and biological consequences were investigated. The lung
weights of the 15.0 and 45.0 mg/kg BW male and female groups were significantly higher
than those of the control groups. The lungs of treated rats showed enlargement and black
patches originating from the color of magnetite. The typical histopathological changes in
the lungs of the treated rats included infiltration of macrophages phagocytosing
magnetite, inflammatory cell infiltration, granuloma formation and an increase of goblet
cells in the bronchial epithelium. The results clearly show that instilled magnetite
causes foreign body inflammatory and granulating lesions in the lung. These pulmonary
responses occur in a dose-dependent manner in association with the increase in lung
weight. 相似文献
118.
Constantinoiu CC Lillehoj HS Matsubayashi M Tani H Matsuda H Sasai K Baba E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):403-408
The characterization of five chicken monoclonal antibodies (mAbs) that were developed against apical complex antigens of Eimeria acervulina sporozoites is realized and the mAbs reactivity to merozoites belonging to this species is tested. Using immuno-fluorescence assay (IFA), one mAb (HE-4) that recognized apical antigens common to sporozoites of E. acervulina and E. brunetti bound antigens localized on the apical tip of merozoites from all stages of development examined. The mAb 8E-1, reactive with antigens found on the apical tip of all chicken Eimeria sporozoites, also showed binding to antigens common to merozoites from all generations. Another mAb, 8C-3, which identified an antigen shared by sporozoites apical tip and sporocysts wall of E. acervulina reacted very weak and inconstantly with the merozoites from all generations whereas the mAbs 5D-11 and 8D-2 that recognized antigens shared by the sporozoites of E. acervulina and E. maxima (mAb 5D-11) and E. acervulina and E. brunetti (mAb 8D-2) did not react with the merozoites from any generation. Collectively, these results showed that the invasive stages of chicken Eimeria share cross reactive apical complex antigens which are inter-species and inter-generation-specific that might be components of a potential recombinant vaccine. 相似文献
119.
Isolation of genes showing increased expression during bovine adipocyte differentiation 总被引:1,自引:0,他引:1
Tadashi YAMASAKI Miho INOUE-MURAYAMA Koji TAHARA Shoichi TAKANO Akinori SUGIYAMA Tomohito ITOH Akiko TAKASUGA Yoshikazu SUGIMOTO Michael T. ROZE Hisashi ASO Shin'ichi ITO 《Animal Science Journal》2005,76(5):479-489
The adipocyte is important not only for the storage of excess energy as fat, but also for the secretion of homeostatic factors. Gene expression profiles during adipocyte differentiation have been reported previously for mouse 3T3‐L1 cells. However, the profiles of adipogenic gene expression in mice and cattle may be different because several metabolic pathways of the ruminant adipose tissue are different from those of non‐ruminants. The gene expression profile in a clonal bovine intramuscular preadipocyte cell line during adipogenesis was examined using the polymerase chain reaction‐subtraction method. Six hundred and twenty‐one clones, which were expressed at an early stage of differentiation, from the preadipocyte to adipocyte, were isolated and characterized. Further detailed studies were carried out for 86 selected genes using northern blotting. Ten genes were found to be highly expressed after differentiation of bovine intramuscular preadipocyte cells. In particular, the expression profiles of genes for stearoyl CoA desaturase and FK506 binding protein were quite different from the time course of differentiation of that seen in the 3T3‐L1 cells reported previously. In addition, these genes were assigned to bovine chromosomes using a bovine/hamster somatic cell hybrid panel and public database. 相似文献
120.
Differential responses of macrophages to Salmonella enterica serovars Enteritidis and Typhimurium 总被引:1,自引:0,他引:1
Okamura M Lillehoj HS Raybourne RB Babu US Heckert RA Tani H Sasai K Baba E Lillehoj EP 《Veterinary immunology and immunopathology》2005,107(3-4):327-335
Macrophages are major effectors against Salmonella infection, and also transport bacteria between host tissues and provide a protected site for intracellular bacterial replication. We hypothesized that differences in chicken macrophage responses to Salmonella enterica serovar Enteritidis (SE) and serovar Typhimurium (ST) played a role in preferential infection of eggs by SE compared with ST. To test this hypothesis, we determined bacterial phagocytosis and intracellular viability and macrophage nitric oxide (NO) production following in vitro infection with SE or ST in the presence or absence of interferon-gamma (IFN-gamma). The effects of bacterial components, lipopolysaccharide (LPS), outer membrane proteins (OMP) and flagella, on NO production were also assessed. Our results showed: (1) in the presence or absence of IFN-gamma, the percentage macrophages phagocytizing SE and ST was similar; (2) the number of intracellular viable SE was significantly reduced compared with ST in the presence or absence of IFN-gamma; (3) increased macrophage necrosis was seen in the presence of IFN-gamma and ST; (4) Salmonella infection acted synergistically with IFN-gamma in induction of nitric oxide production; and (5) in the absence of IFN-gamma, macrophages produced significantly greater NO following treatment with SE outer membrane protein or flagella compared with ST OMP or flagella, while in the presence of IFN-gamma significantly less NO was produced following treatment with SE-LPS compared with ST-LPS. These results suggest that differential responses of chicken macrophages to SE versus ST may result in increased macrophage death with ST, which could result in an increased inflammatory response as compared to SE. 相似文献