百脉根种子硬实处理与耐藏性的探讨

地百脉根种子硬实用不同方法处理和对不同贮藏时间种子耐藏性的测定结果表明：贮藏１年种子含硬实率５９．３％。经擦破种皮或硫酸浸种处理，硬实率明显下降，发芽势，发芽率显著提高；种子随贮藏期的增长，硬实率明显下降，其发芽势，发芽率，活力指数以及直播出苗率，幼细苗存活率等亦随贮藏时间的延长而下降；种子电导值则随贮藏时间的延长而增大。     

Eastern equine encephalitis in a flock of emus (Dromaius novaehollandiae).

Eastern equine encephalitis (EEE) was diagnosed in a flock of emus in southeastern Louisiana. The outbreak involved juvenile and adult breeders ranging in age from 20 to 36 months, with an attack rate of 76% and a case fatality rate of 87%. The diagnosis was confirmed by isolation and characterization of the viral agent, and by detection of EEE antibody in two recovered emus. High mortality was preceded by marked depression, hemorrhagic diarrhea, and emesis of blood-stained ingesta. On postmortem examination, hemorrhagic enteritis and multiple petechia of viscera were observed. Microscopic changes included severe necrosis of hepatocytes, intestinal mucosa, and necrotizing vasculitis of the spleen and lamina propria of the intestine. No nervous system lesions were observed. This outbreak occurred concurrently with EEE in horses and was attributed to unseasonably heavy rainfall with an abundance of arthropod vectors and proximity to free-living reservoir host species.     

大豆室内耐冷筛选及其在田间的应用研究

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。     

Toxic peripheral neuropathy with demyelination in Sprague-Dawley rats given CGS 21595--a 5-lipoxygenase inhibitor.

Male and female Sprague-Dawley rats were given CGS 21595, a pro-drug that is almost immediately metabolized to CGS 19213, a naphthoquinone that acts as a 5-lipoxygenase inhibitor. The compound was administered by gavage to five groups of Sprague-Dawley rats (group Nos. 1, 5, n = 30; group Nos. 2-4, n = 20) at daily doses of 0, 50, 150, 500, or 1,000 mg/kg for 13 weeks. Rats in the higher dose groups had a reduced weight gain, but significant neurologic signs were not observed. A peripheral neuropathy consisting predominantly of myelin destruction in the spinal nerve roots and sciatic nerves in male rats treated with greater than or equal to 150 mg/kg CGS 21595 and in female rats treated with greater than or equal to 50 mg/kg CGS 21595 for 13 weeks. This lesion was not fully reversible after a recovery period of 4 weeks. Lesions consisted of ballooning of myelin sheaths, infiltration by macrophages, demyelination, and occasional areas of remyelination. Axons were generally preserved, and the brain and spinal cord were not affected. Male and female rats in all treatment groups had cytoplasmic hyaline droplets in the proximal renal tubules. This change was reversible after 4 weeks and was not associated with any other adverse effects on the kidney.     

台湾农业及农村发展经验考察

作者受中国农村发展规划学会邀请,赴台进行为期1个月的研究学习,目的是了解和考察台湾农业及农村发展的经验与历程。一个月的考察和学习使作者对台湾农业生产、农产品运销、农技推广、农业组织体系、农村居住环境等诸多方面有了切实的感性体会。     

三种不同方法显示仔猪小肠肌间神经元的比较

本研究采用小肠铺片NADPH-d组化法、NADH-d组化法和抗NF免疫组化法分别对0日龄、5日龄、28日龄仔猪的肌间神经元进行了比较研究。结果表明:NADPH-d组化法可以选择性的标记合成NO的神经元亚群,大部分阳性肌间神经元符合DogielI型神经元的形态特征;NADH-d组化法可以非选择性的标记大部分神经元,且采用该法观察到的肌间神经元的密度明显高于NADPH-d组化法,但该法不能清晰的显示肌间神经丛的细微结构,不适合用于神经元类型的观察;抗NF免疫组化法可以清晰的显示肌间神经元的突起,28日龄仔猪体积较大的阳性神经元主要为DogielI型和DogielII型神经元。     

Manipulated Mouse Embryos as Bioassay System for Water Quality Control

Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K⁺ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system.     

2005年农业部第四检查组兽药执法检查情况报告

根据国务院《兽药管理条例》和农业部《2005年兽药市场专项整治工作方案》，为检查督导兽药市场整治工作，受农业部兽医局委派，兽药执法检查组一行4人于2005年6月3日至6月12日，先后对广东、广西、湖南3省兽用生物制品供应体系以及14个市（县）、13个兽药生产企业、20多个兽药经营单位、7个兽药使用单位、3个科研教育单位等进行了系统、全面的检查，现将3省市有关情况和检查中发现的问题汇总如下：     

VA菌根对茄子、黄瓜、籽瓜促生防病效应的研究

在盆栽条件下研究了ＶＡ菌根茵对茄子、黄瓜、籽瓜的侵染、促生和防病作用。结果供试菌均可侵染供试作物，但促生和防病效应具特异性．Ｇｌｏｍｕｓ．Ｖｅｒｓｉｆｏｒｍｅ和Ｇ．ｍｏｓｓｅａｅ均促进茄子的生长，但只前者减轻了茄子黄萎病。Ｇ，ｓｐ３促进黄瓜提早开花，但不防病；Ｇ．ｍａｃｒｏｃａｒｐｕｍ减轻了黄瓜细菌性角斑病的发生，却并无促生作用。Ｇ．ｓｐ４不但促进籽瓜的生长，而且是短期内减轻籽瓜炭疽病的有效组合。