口蹄疫病毒A型竞争ELISA抗体检测试剂盒在流行病学调查中的应用

为评价口蹄疫病毒A型竞争ELISA(cELISA)抗体检测试剂盒在流行病学调查中的应用前景,对2017年从福建省三明市采集的336份黄牛、奶牛、羊和猪血清样品,用A型cELISA抗体检测试剂盒进行抗体检测。结果显示,92份黄牛血清、92份羊血清、92份猪血清、60份奶牛血清的A型抗体阳性率分别为13.04%、11.96%、20.65%、86.67%。从上述4种血清中,各挑选10份血清(阴性、阳性各5份)共40份,采用口蹄疫病毒液相阻断ELISA(LPB-ELISA)抗体检测试剂盒进行验证。结果显示:cELISA检测为阳性的20份血清中,用LPB-ELISA检出阳性19份;cELISA检测为阴性的20份血清中,用LPB-ELISA检出阴性17份;两种方法的κ值为0.8,总符合率为90.00%。结果表明,A型cELISA试剂盒与LPB-ELISA试剂盒的符合率和一致性均较高,可用于口蹄疫流行病学调查和血清学监测。     

超额储备后备母猪的运作模式——非洲猪瘟背景下集团化养猪企业的盈利之道

集团猪场代表行业最高技术、管理及现代化水平,迅速发展。特别是2018年非洲猪瘟疫情在我国暴发后,在高风险、高回报的行业背景下,集团化养猪企业凭借资金、人才、技术、硬件设施等优势,采取超额储备种猪的运作模式,进而迅速扩张。超额储备的本质是利用资金充足、场区数量、生物安全等优势,通过统一使用长白或大白公猪配种,育肥场用作种猪场等方式超额储备后备母猪。个别场区清场后可以迅速引种补栏,多余后备母猪、妊娠母猪正常出售回收资金。这种模式可有效降低风险、保证足够盈利,同时在非洲猪瘟疫情的背景下有效整合农业资源、利用商业资金反哺养猪业,指引养猪行业走向。     

Analysis of Genotype,Environment, and Their Interaction Effects on the Phytochemicals and Antioxidant Capacities of Red Rice (Oryza sativa L.)

Fourteen red rice varieties were planted in two locations during summer (Hangzhou) and winter (Hainan) to study the effect of genotype and environment on the phytochemicals and antioxidant capacities of rice grain. B‐type proanthocyanidins in red rice were detected by LC‐MS/MS and quantified by using the vanillin assay. Analysis of variance showed that total phenolic content (TPC), total flavonoid content (TFC) and 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radical scavenging capacity were mainly affected by environmental factors, which accounted for more than 60% of the total variance. However, total proanthocyanidin content (TPAC) and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging capacity were equally affected by both genotype and environment. The genotype × environment effects were significant for all traits. The pairwise correlations among TPC, TFC, TPAC, ABTS, and DPPH were also significant (r > 0.900, P < 0.001). Principal component analysis identified the genotypes that had higher contents of antioxidants and more stability across environments. This study showed that indirect selection of a simple trait (i.e., TPC) is an effective way to select rice high in antioxidant capacity in breeding programs. This study also suggests that rice should be produced specifically in a certain environment for the end user to minimize the variation in the functional properties and maximize their contents.     

穗肥施用时期对水稻籽粒中胚乳蛋白积累的影响

以武香粳9号为材料研究了不同穗肥施氮时期对水稻籽粒蛋白质积累的影响.结果表明:穗肥的施用提高了籽粒发育过程中单位干重蛋白质含量,以在倒0.5叶施用氮素穗肥影响最大;随施肥时期的延迟,稻米粗蛋白和谷蛋白含量逐步上升,醇溶蛋白含量逐渐降低;倒2.5叶施氮减少了强、弱势粒中蛋白组分的分布和含量,倒0.5叶施氮与此相反;胚乳     

抗草甘膦棉花研究进展

抗草甘膦棉花最早于1997在美国释放,它是通过将编码抗草甘膦酶5-烯醇式丙酮酰莽草酸-3-磷酸合成酶基因导入珂字棉312而获得的抗性株.抗草甘膦棉花在美国的种植面积占棉花总种植面积从1997年的3.2%到增加到2005年的60%左右.在这近十年内,抗草甘膦棉花虽然得到了广泛的种植,但也存在着不少问题,其中最为突出的是草甘膦的施用方法和时期的不正确操作对抗草甘膦棉花的根系生长、育性和产量都产生了负面影响.这主要是因为:1、外界因素,包括草甘膦施用方法在内的其他栽培措施和不利的环境条件,如高温,对抗草甘膦棉花的产量及生长的影响;2、内部因素,抗草甘膦棉花不同器官对草甘膦的吸收量及抗草甘膦基因的表达量差异有关.因此,本文从抗草甘膦棉花对草甘膦的吸收分配、草甘膦的施用量和时期对抗草甘膦棉花根系生长、花药开裂及产量等方面综述了这些年来抗草甘膦棉花在应用过程中的问题,并且对今后抗草甘膦棉花育种方面作了展望.     

New Progression about Gravity Quantization in General Relativity Theory in Real Space-time

Professor Venzo de Sabbata,working at World Laboratory,Lausanne,Switzerland,and Department of Physics,University of Bologna and Ferrara,Italy,is a famous Italy Physicist in the world.He is engaged with a series of creating research works about gravity quantization of General Relativity Theory in the real space-time which attract the attentions of the world.This paper pays the attention to introduce the works of this field,containing mainly:1.quantizing the general relativity theory in the sense of Einstem-Cartan theory,i.e., general relativity adds the torsion,which means introducing spin into the theory of gravity.2. building a compact mathematical tool for research of gravity quantization,the geometric algebra in the real space-time--multivector algebra,i.e.,developing a spinor manifold in the real space-time.     

A Comparative Study of Confined Concrete Models under Cyclic Loading

In this paper,four models are selected as the representatives and applied to nonlinear dynamic analysis program for space frames which is based on the beam-column elements using the finite element flexibility method and the fiber model.Park's test on combined axial and bending columns under cyclic loading are taken as the calibrations,comparisons to material and structural member level are drawn and the analytical results of various models are discussed.     

Solid Model Design on the Features of Products

To research the problem of plastic art design and emulation of products with the virtual reality technology can short the product development cycle and reduce the cost. This paper discusses the information based on the product feature ,solid model with parameters, the structure and function of this system. Combined the product properties with its solid model. Proposed a method with CAD to create the model of the product based on features in the product design. Expressed its feature parameter and shape of model fully .In the case for a deceleration box the feature model has solved the problem of physical information ,geometry information and the informative process in its production management system efficiently.