全文获取类型
收费全文 | 150篇 |
免费 | 13篇 |
专业分类
林业 | 15篇 |
农学 | 6篇 |
基础科学 | 1篇 |
30篇 | |
综合类 | 24篇 |
农作物 | 8篇 |
水产渔业 | 5篇 |
畜牧兽医 | 61篇 |
园艺 | 1篇 |
植物保护 | 12篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 8篇 |
2015年 | 4篇 |
2014年 | 5篇 |
2013年 | 13篇 |
2012年 | 11篇 |
2011年 | 14篇 |
2010年 | 4篇 |
2009年 | 4篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 2篇 |
2005年 | 9篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1964年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有163条查询结果,搜索用时 19 毫秒
11.
Shahzad Ali Amanullah Jan Jia Zhikuan Amir Sohail Cai Tie Wei Ting Zhang Peng Manzoor Irshad Ahmad Mati Ur Rahman Ren Xiaolong Liu Xiaoli Xu Yue Yue 《Russian Agricultural Sciences》2016,42(3-4):224-229
A field experiment was conducted on silty clay loam soil in the years 2011–2012. Two sesame (Sesamum indicum L.) cultivars (Local Black and Local White) were evaluated using various 3 different sowing dates (20th June, 10th and 30th July) and four agrotechnical level (0, 40, 80 and 120 kg N ha–1) at New Developmental Farm The University of Agriculture, Peshawar, Pakistan. The objective of this study was to evaluate the effects of sowing dates on growth, yield and oil fatty acid composition of two sesame cultivars grown under different nitrogen fertilization. Results showed that cv. Local Black was characterized by significantly higher content of oil (47%), seed yield (696 kg ha–1) and oil yield (335 kg ha–1) while cv. Local White had higher palmitic acid (8%) and linoleic acid (38.7%). Yield and its main components were positively affected by the earlier sowing date. With regard to fatty acid composition, a decrease in oleic and stearic acid and an increase in linoleic and palmitic acid were observed. At early sowing, oleic and palmitic acid decreased whereas linoleic and stearic acid increased. The decrease in the oleic/linoleic acid ratio observed at early sowing, suggests a possible role of temperature on the activity of oleate desaturase in the developing seeds. Intensive technology of cultivation (120 kg N ha–1), compared to the economical technology (40 kg N ha–1), significantly increased the seed yield of both sesame cultivars. This was due to higher number of branches, Capsules m–2, capsules plant–1, seeds capsule–1 and 1000 seed weight (g). The intensive technology of cultivation had a beneficial effect on the content of palmitic acid, linolenic acid and oleic acid in sesame seed. 相似文献
12.
Du J Zhou Y Su X Yu JJ Khan S Jiang H Kim J Woo J Kim JH Choi BH He B Chen W Zhang S Cerione RA Auwerx J Hao Q Lin H 《Science (New York, N.Y.)》2011,334(6057):806-809
Silent information regulator 2 (Sir2) proteins (sirtuins) are nicotinamide adenine dinucleotide-dependent deacetylases that regulate important biological processes. Mammals have seven sirtuins, Sirt1 to Sirt7. Four of them (Sirt4 to Sirt7) have no detectable or very weak deacetylase activity. We found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro. The preference for succinyl and malonyl groups was explained by the presence of an arginine residue (Arg(105)) and tyrosine residue (Tyr(102)) in the acyl pocket of Sirt5. Several mammalian proteins were identified with mass spectrometry to have succinyl or malonyl lysine modifications. Deletion of Sirt5 in mice appeared to increase the level of succinylation on carbamoyl phosphate synthase 1, which is a known target of Sirt5. Thus, protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo. 相似文献
13.
Sorek R Zhu Y Creevey CJ Francino MP Bork P Rubin EM 《Science (New York, N.Y.)》2007,318(5855):1449-1452
Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to that of another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we studied the attempted movement of 246,045 genes from 79 prokaryotic genomes into Escherichia coli and identified genes that consistently fail to transfer. We studied the mechanisms underlying transfer inhibition by placing coding regions from different species under the control of inducible promoters. Our data suggest that toxicity to the host inhibited transfer regardless of the species of origin and that increased gene dosage and associated increased expression may be a predominant cause for transfer failure. Although these experimental studies examined transfer solely into E. coli, a computational analysis of gene-transfer rates across available bacterial and archaeal genomes supports that the barriers observed in our study are general across the tree of life. 相似文献
14.
To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins. 相似文献
15.
Zdobnov EM von Mering C Letunic I Torrents D Suyama M Copley RR Christophides GK Thomasova D Holt RA Subramanian GM Mueller HM Dimopoulos G Law JH Wells MA Birney E Charlab R Halpern AL Kokoza E Kraft CL Lai Z Lewis S Louis C Barillas-Mury C Nusskern D Rubin GM Salzberg SL Sutton GG Topalis P Wides R Wincker P Yandell M Collins FH Ribeiro J Gelbart WM Kafatos FC Bork P 《Science (New York, N.Y.)》2002,298(5591):149-159
Comparison of the genomes and proteomes of the two diptera Anopheles gambiae and Drosophila melanogaster, which diverged about 250 million years ago, reveals considerable similarities. However, numerous differences are also observed; some of these must reflect the selection and subsequent adaptation associated with different ecologies and life strategies. Almost half of the genes in both genomes are interpreted as orthologs and show an average sequence identity of about 56%, which is slightly lower than that observed between the orthologs of the pufferfish and human (diverged about 450 million years ago). This indicates that these two insects diverged considerably faster than vertebrates. Aligned sequences reveal that orthologous genes have retained only half of their intron/exon structure, indicating that intron gains or losses have occurred at a rate of about one per gene per 125 million years. Chromosomal arms exhibit significant remnants of homology between the two species, although only 34% of the genes colocalize in small "microsyntenic" clusters, and major interarm transfers as well as intra-arm shuffling of gene order are detected. 相似文献
16.
Goodman LA Jarrett CL Krunkosky TM Budsberg SC Northrup NC Saba CF LeRoy BE 《Veterinary and comparative oncology》2011,9(2):149-157
5-Lipoxygenase (5-LO) is overexpressed in human prostate carcinomas (PCs), and its inhibition decreases proliferation and induces apoptosis in prostate cancer cell lines. We hypothesized that 5-LO would be overexpressed in canine PC compared with benign prostate tissue and may be important in the pathogenesis of the disease. Immunoblot analysis of canine PC and benign prostatic hyperplasia (BPH) tissues demonstrated 5-LO expression in both. 5-LO immunohistochemical staining was not significantly different within the stromal or epithelial components of canine primary PC, BPH or suppurative prostatitis, suggesting that differential expression of this enzyme does not occur in these conditions. The percentage of tumour cells expressing 5-LO was significantly lower in metastatic PC lesions compared with primary PC (P < 0.0001). This decreased expression may indicate down-regulation or altered expression of the enzyme with progression of canine PC to a metastatic phenotype. 相似文献
17.
With a world‐wide occurrence on about 560 million hectares, sodic soils are characterized by the occurrence of excess sodium (Na+) to levels that can adversely affect crop growth and yield. Amelioration of such soils needs a source of calcium (Ca2+) to replace excess Na+ from the cation exchange sites. In addition, adequate levels of Ca2+ in ameliorated soils play a vital role in improving the structural and functional integrity of plant cell walls and membranes. As a low‐cost and environmentally feasible strategy, phytoremediation of sodic soils — a plant‐based amelioration — has gained increasing interest among scientists and farmers in recent years. Enhanced CO2 partial pressure (PCO2) in the root zone is considered as the principal mechanism contributing to phytoremediation of sodic soils. Aqueous CO2 produces protons (H+) and bicarbonate (HCO3‐). In a subsequent reaction, H+ reacts with native soil calcite (CaCO3) to provide Ca2+ for Na+ Ca2+ exchange at the cation exchange sites. Another source of H+ may occur in such soils if cropped with N2‐fixing plant species because plants capable of fixing N2 release H+ in the root zone. In a lysimeter experiment on a calcareous sodic soil (pHs = 7.4, electrical conductivity of soil saturated paste extract (ECe) = 3.1 dS m‐1, sodium adsorption ratio (SAR) = 28.4, exchangeable sodium percentage (ESP) = 27.6, CaCO3 = 50 g kg‐1), we investigated the phytoremediation ability of alfalfa (Medicago sativa L.). There were two cropped treatments: Alfalfa relying on N2 fixation and alfalfa receiving NH4NO3 as mineral N source, respectively. Other treatments were non‐cropped, including a control (without an amendment or crop), and soil application of gypsum or sulfuric acid. After two months of cropping, all lysimeters were leached by maintaining a water content at 130% waterholding capacity of the soil after every 24±1 h. The treatment efficiency for Na+ removal in drainage water was in the order: sulfuric acid > gypsum = N2‐fixing alfalfa > NH4NO3‐fed alfalfa > control. Both the alfalfa treatments produced statistically similar root and shoot biomass. We attribute better Na+ removal by the N2‐fixing alfalfa treatment to an additional source of H+ in the rhizosphere, which helped to dissolve additional CaCO3 and soil sodicity amelioration. 相似文献
18.
Clarke E. Atkins Ronald Kanter Kathy Wright Ziad Saba Catherine Baty Cliff Swanson Stephen Bai Bruce W. Keene 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(1):43-49
A 4-month-old male Labrador Retriever was presented for recurrent bouts of pulmonary edema associated with tachycardia. Initial physical examination and echocardiography were unremarkable, and the electrocardiogram revealed only an intraventricular conduction disturbance. Subsequent recordings showed paroxysmal supraventricular tachycardia (SVT) (340 beats/min), which consistently produced pulmonary edema. The supraventricular tachycardia was unresponsive to adenosine, esmolol, and propranolol; was variably and transiently responsive to various vagal maneuvers and precordial thumps; and was always responsive to IV diltiazem. Multiple life-threatening episodes of SVT occurred, however, despite the chronic administration of oral diltiazem, propranolol, and procainamide. Diastolic cardiac dysfunction was documented by Doppler echocardiography and was thought to contribute to the development of pulmonary edema. A subsequent electrophysiologic study confirmed the presence of an atrioventricular posteroseptal accessory pathway that participated in orthodromic reciprocating tachycardia. This pathway was determined to conduct only in the retrograde direction ("concealed accessory pathway"). Intraoperative IV procainamide titration terminated the arrhythmia, which could not be reinduced when procainamide blood concentration approximated 20 μg/dL. Increasing the oral procainamide dose to achieve such plasma concentrations was successful in eliminating orthodromic reciprocating tachycardia, preventing heart failure, and returning Doppler indices of diastolic function to normal. 相似文献
19.
While the respiratory quotient (R.Q.) of Rhinomugil corsula in air-saturated water remains near unity at all levels of random activity, the ammonia quotient (A.Q. = volume or mole: mole relation of ammonia excreted to oxygen consumed) is higher at lower levels of activity, indicating relatively higher protein utilization in less active fish. The mean routine R.Q. values of R. corsula in air-saturated water at temperatures of 30 and 35°C are 0.91 and 0.95 respectively. Assuming that the major sources of ammonia excreted are certain predominant free amino acids, an estimate of caloric break-up of routine meta-bolism of R. corsula suggests that the fish is completely aerobic in air-saturated water and that it derives 14, 44 and 42% of energy from proteins, carbohydrates and fats respectively from a total of 567 cal/kg fish/h at 30°C (15, 55 and 30% at 35°C). At oxygen concentrations below air saturation, R.Q. and A.Q. values of R. corsula increase with decrease in ambient oxygen indicating an increase in anaerobic metabolism and protein utilization. The correlation between the trends of R.Q. and A.Q. levels are similar to that observed earlier in Tilapia mossambica. During hypoxia (1.6 mg O2/l at 30°C) R. corsula derives about half its energy for sustenance and activity anaerobically. The mullet does not appear to accumulate an oxygen debt during hypoxia. 相似文献
20.
Seroprevalence of leptospiral antibodies among dairy cattle kept in communal corrals in periurban areas of Bamako, Mali, West Africa 总被引:1,自引:0,他引:1
Mamadou Niang Loren A. Will Mamadou Kane Alpha A. Diallo Manzoor Hussain 《Preventive veterinary medicine》1994,18(4):259-265
Sera obtained from dairy cattle in communal corral herds located near the city of Bamako in Mali, West Africa were sceeened using the microscopic agglutination test against 26 serovars representing 15 serogroups of Leptospira interrogans. Forty five percent (44.8%; 288/642) were positive to one or more serovars. This seroprevalence rate likely reflects infection since vaccination against leptospiral infection has not been practiced i Mali. The rate was higher among females. The most common titers were against antigens of serovars cynopteri (14.7%), hardjo (13.3%), pomona (9.2%), nona (8.1%), kambale (6.5%), grippotypyosa (6.4%), hebdomadis (6.2%), and pyrogenes (5.2%). 相似文献