全文获取类型
收费全文 | 70篇 |
免费 | 4篇 |
国内免费 | 2篇 |
专业分类
林业 | 3篇 |
农学 | 4篇 |
12篇 | |
综合类 | 11篇 |
农作物 | 10篇 |
水产渔业 | 9篇 |
畜牧兽医 | 20篇 |
植物保护 | 7篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 7篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 9篇 |
2016年 | 3篇 |
2013年 | 5篇 |
2012年 | 3篇 |
2011年 | 1篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 1篇 |
2007年 | 4篇 |
2004年 | 1篇 |
2003年 | 2篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1997年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1981年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有76条查询结果,搜索用时 31 毫秒
31.
32.
Jyotsna Parameswaran Vijayakumar Ravi Mani Sudhakaran Raja Tharmathass Stalin Dhas 《Journal of fish diseases》2020,43(2):263-273
In the present study, a new cell line from the vertebra of mosquitofish Gambusia affinis was successfully established and characterized. The cell line is named as bone Gambusia affinis (BGA) and subcultured for more than 55 passages in Leibovitz's/L15 medium supplemented with 15% FBS at 28°C. The cell line has a modal chromosome number of 48. Molecular characterization of the partial sequence of the coi gene confirmed the origin of the BGA cell line from mosquitofish. These cells exhibited epithelial morphology confirmed by the cytokeratin marker. The BGA cells showed mineralization of their extracellular matrix when stained with alizarin red and von Kossa stain. BGA cells were found to be susceptible to RGNNV and SJNNV strains of betanodavirus (NNV) showing cytopathic effect with multiple vacuolations in the cells. The RT-PCR confirmed the betanodavirus infections in BGA cells. The SEM micrograph showed the morphological changes observed in the cell during virus infection. The in vivo challenge experiment also showed the viral replicating efficiency in the Gambusia affinis with increasing viral titre. Thus, our present results show that the BGA cell line is a useful tool for isolating betanodavirus and could be used to investigate bone cell differentiation and extracellular matrix mineralization. 相似文献
33.
Akshaya Panigrahi Mani Sundaram Saranya Chakrapani Satishkumar Rajasekar Jagabattula Syama Dayal Gopal Chavali 《Aquaculture Research》2019,50(1):29-41
A 16‐week indoor culture trial was conducted to evaluate the effect of varying C:N ratio on growth performance, physico‐chemical parameters, microbial dynamics, feed utilization, and immunological parameters. The experiment comprised of five biofloc treatment groups (with varying C:N ratio 5:1, 10:1, 15:1, 20:1) and a control with three replicates each, having 100 nos/m3 as stocking density in 500 L tanks with constant aeration. The C:N ratios of the treatments were manipulated using molasses as an organic carbon source whereas there was no carbon source added in control. The water quality parameters monitored throughout the experiment were found to be within permissible limits in shrimp culture. At the end of the experiment, it was observed that there were significant differences between the treatment groups and the control regarding absolute growth, SGR, FCR, PER, and FER. Furthermore, a considerable difference in immunological parameters, namely, THC, phagocytosis, and PO activity (17.5 × 106 cells per ml, 43.5%, 0.112 Units min?1 mg min?1), was recorded among the treatments compared to that of the control groups (6.2 × 106 cells per ml, 31.5%, 0.051 Units min?1 mg min?1) respectively. Enhanced growth and survival with substantial disease resistance were recorded in C15 treatment. The results indicate that the CN15 ratio coupled with minimal water exchange is optimal for improved survival, growth, and immune activity. 相似文献
34.
Gregory F. Grauer Deborah S. Greco Ellen N. Behrend Indu Mani Martin J. Fettman Timothy A. Allen 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(5):324-327
The correlation between 24-hour urinary excretion of N -acetyl-β- d -glucosaminidase (NAG) and γ-glutamyl transferase (GGT) with urine NAG and GGT/creatinine ratios was assessed in dogs with gentamicin-induced nephrotoxicosis. Eighteen 6-month-oid male Beagles with normal renal function were randomly divided into 3 groups of 6. Each group was fed a different concentration of protein (high protein, 27.3%; medium protein, 13.7%; and low protein, 9.4%) for 21 days. After dietary conditioning, gentamicin was administered at a dose of 10 mg/kg IM tid for 8 days and each group was continued on its respective diet. Endogenous creatinine clearance and 24-hour urinary excretion of NAG and GGT were determined after dietary conditioning (day 0) and on days 2, 4, 6, and 8 of gentamicin administration. In addition, urine NAG and GGT/creatinine ratios (IU/L ± mg/dL) were determined from catheterized spot urine samples obtained between 7 and 10 am on the same days. The correlation between 24-hour urinary enzyme excretion and urine enzyme/creatinine ratio in the spot urine samples was evaluated by simple linear regression analysis. Spot sample urine enzyme/creatinine ratios were significantly correlated with 24-hour urinary enzyme excretion through day 4 for dogs on low dietary protein, through day 6 for those on medium protein, and through day 8 for those on high dietary protein. Mean ± SD baseline values for urine NAG/creatinine ratio and 24-hour urinary NAG excretion were 0.06 ± 0.04 and 0.19 ± 0.14 IU/kg/24 hr, respectively. Baseline values for urine GGT/creatinine ratio and 24-hour urinary GGT excretion were 0.39 ± 0.18 and 1.42 ± 0.82 IU/kg/24 hr, respectively. 相似文献
35.
E. Cacciuttolo G. Rossi S. Nardoni R. Legrottaglie P. Mani 《Veterinary research communications》2009,33(6):521-527
Aspergillosis is a fungal disease caused by fungi of the genus Aspergillus, in particular A. fumigatus and A. flavus. This paper focuses on anatomopathological aspects resulting from a chronic infection from Aspergillus spp in the chicken (Gallus domesticus), in the herring gull (Larus cachinnans micaelli) and in the red-legged partridge (Alectoris rufa rufa). Microscopically, we observed some histological lesions that are related to the two typical forms of Aspergillosis: a deep
nodular form, typical of organs with a non-aerated parenchyma, and a non-encapsulated superficial diffuse form typical of
the serosae and the lung. The observed forms of aspergillosis have been found in animals raised in poor hygienic environmental
conditions or malnourished animals (chicken); in wild birds from wildlife recovery centres (herring gull), which underwent
some forms of stress, such as traumas, detention, starvation, extended antibiotic treatments; in game birds (red-legged partridge)
used for restocking natural areas that had been negatively affected by such stressors as captivity in aviaries, containment
and transport in cages, release in unsuitable environments and malnutrition. The observed anatomopathological and istopathological
aspects can therefore be regarded as the outcome of a number of factors that have reduced the typical resistance of the species
and impaired the efficiency of their immune systems. 相似文献
36.
Peter Mani 《中国家禽》2007,29(20):24-27
1 管理结构
设施管理组的主要任务:明确角色分配,明确传达信息,明确权限分配的安全管理方案.…… 相似文献
37.
Yamada K Lim J Dale JM Chen H Shinn P Palm CJ Southwick AM Wu HC Kim C Nguyen M Pham P Cheuk R Karlin-Newmann G Liu SX Lam B Sakano H Wu T Yu G Miranda M Quach HL Tripp M Chang CH Lee JM Toriumi M Chan MM Tang CC Onodera CS Deng JM Akiyama K Ansari Y Arakawa T Banh J Banno F Bowser L Brooks S Carninci P Chao Q Choy N Enju A Goldsmith AD Gurjal M Hansen NF Hayashizaki Y Johnson-Hopson C Hsuan VW Iida K Karnes M Khan S Koesema E Ishida J Jiang PX Jones T Kawai J Kamiya A Meyers C Nakajima M 《Science (New York, N.Y.)》2003,302(5646):842-846
38.
Qing-xia Dai Noriharu Ae Takeshi Suzuki Mani Rajkumar Shoko Fukunaga Nobuhide Fujitake 《Soil Science and Plant Nutrition》2013,59(4):500-507
A five-step sequential extraction procedure was developed for the partitioning of soil aluminum (Al) into five fractions: exchangeable, weakly organic bound, strongly organic bound, inorganic non-crystalline and crystalline. The results obtained by the sequential extraction method for Al were compared with those estimated from single extractions using identical operating conditions applied in each individual sequential fraction. The Al content in the extracts was measured by inductively coupled plasma optical emission spectrometry. The results indicated that the first four steps [potassium chloride (KCl), copper chloride (CuCl2), sodium pyrophosphate (Na4P2O7), acid ammonium oxalate ((NH4)2C2O4)] in the sequential procedure could be as effective as single extraction methods at estimating exchangeable Al, weakly organic bound Al, strongly organic bound Al, and non-crystalline Al. However, the crystalline Al content by sequential procedure was not in agreement with single extraction procedures. Further, the sequential extractions resulted in more consistent estimates of the aluminum/silicon (Al/Si) molar ratio and allophane and crystalline Al contents than single extractions method. Results of X-ray diffraction on the soil samples confirm the presence of appreciable amounts of at least three types of crystalline minerals, including gibbsite, vermiculite or halloysite. Our result implies that the sequential method can be more reliable in estimating the various Al forms in Andisols. 相似文献
39.
Tulasi leaf powder was fed at the 1% level in normal and diabetic rats for a period of one month to explore the effect on
fasting blood sugar, uronic acid, total amino acids, and the lipid profile in serum and tissue lipids. The results indicated
a significant reduction in fasting blood sugar, uronic acid, total amino acids, total cholesterol, triglyceride, phospholipids
and total lipids. In liver, total cholesterol, triglyceride and total lipids were significantly lowered. Total lipids were
significantly reduced in kidney. In heart, a significant fall in total cholesterol and phospholipids was observed. All these
observations indicate the hypoglycemic and hypolipidemic effect of Tulasi in diabetic rats. 相似文献
40.
Mani Ram Moktan Georg Gratzer William H. Richards Tek Bahadur Rai Dawa Dukpa 《Forest Ecology and Management》2009
We examined the regeneration and structure of mixed conifer forests under single-tree harvest management in western Bhutan. Sixteen 900 m2 (30 m × 30 m) plots were sampled at four Forest Management Units (FMUs; Chamgang, Gidakom, Paro-Zonglela, and Haa-East) representing the forest type, including half the plots in single-tree harvest stands and half in unlogged stands. In addition, we solicited information on traditional forest management practices from informants using survey questionnaires and collected tree species data from felling records from respective local forest offices. Rural timber demand is concentrated on the removal of straight and well-formed bluepine trees for beams, planks, and scaffolding. Single-tree harvest, however, has not significantly altered stand structures from unlogged stands. Similarly, tree regeneration is not different when comparing single-tree harvest and unlogged stands, except at Chamgang FMU, where seedling densities were generally higher in harvested stands than in unlogged stands. These results indicate that single-tree harvest is not detrimental to regeneration and utilization of mixed conifer forests in western Bhutan. 相似文献