性信息素诱捕防治棉红铃虫

1980～1981年在浙江海盐和上虞进行了性信息素诱捕防治棉铃虫的大田试验。面积分别为3500、3000亩。试验证明,每亩设置3～4只诱捕盆的大面积诱捕防治能明显降低田间红铃虫雌蛾的交配率(下降68.2～96.7%),棉株受害程度也减轻,与常规农药防治的对照相比,花害率下降42.4～78.9%,铃害率下降30.3～54.4%,同时还减少了用药次数,间接起到保护天敌、减少环境污染的作用。因此认为,性信息素诱捕法有可能作为棉红铃虫综合防治中的一项有效措施。     

Study on the Anti-Escherichia coli Activity and Mechanism of Water Extract of Radix isatidis Powder

In order to explore the antibacterial activity and mechanism of the Radix isatidis powder water extract,the effects of the Radix isatidis powder water extract on the morphology and structure of Escherichia coli (E.coli) were tested by scanning electron microscopy and transmission electron microscopy.The effects of the Radix isatidis powder water extract on the conductivity and total leakage rate of E.coli and the content of alkaline phosphatase in the culture medium of the content of protein,DNA and RNA were stained by DAPI to detect the effect of the Radix isatidis powder water extract on the nucleic acid of E.coli,and the effect of the Radix isatidis powder water extract on the metabolism of E.coli in vivo,in vitro,ALT,AST,pyruvic acid and ATP.The results showed that after the Radix isatidis powder water extract acted on E.coli for 10 h,it was observed by SEM that the bacteria appeared to overflow and shrink,the length of the bacteria became shorter obviously,many residues were formed due to breakage,some of them were sunken in the middle and deformed.Under TEM,it was observed that the boundary of the cell wall of E.coli was unclear,the wall membrane was zigzag,deformed and some of the bacteria were broken.The protein content outside the cell was significantly different from that in the blank control group from 8 h (P<0.01), and that in the cell from 4 h (P<0.01). DNA content had no significant difference with blank control group before 12 h (P>0.05), but had significant difference with blank control group from 16 h (P<0.01); RNA content began to decrease at 8 h, and was significantly different from blank control group at 12 h (P<0.05), and was extremely significant from 16 h (P<0.01). There was no significant difference between ALT and AST (P>0.05). The pyruvate content in culture medium and bacteria was higher than that in blank control group, and the difference was very significant from 4 h (P<0.01). The ATP content in the culture medium was significantly different from that in the blank control group (P<0.01), and the ATP content in the cell was significantly different from that in the blank control group from 4 h (P<0.01).In conclusion,the Radix isatidis powder water extract could inhibit the synthesis and metabolism of bacterial genetic material and the content of pyruvate and ATP by destroying the integrity of cell wall and cell membrane.     

赴美采购流产大豆价格变数加大

2009年5月27日，商务部组团赴美采购2000万吨大豆的“超级订单”，最终流产。双方没有像往年一样签订协议，一个重要原因是中方的报价没有得到美方的认同。而幕后的原因是，美方忌惮我国购买大豆用于收储，调控市场价格。     

普洱茶对被动吸烟小鼠生长性能和血液指标的影响

研究普洱茶对被动吸烟小鼠生长性能和血液指标的影响。180只小鼠分为3组,空白组不吸烟、灌服生理盐水;试验Ⅰ组吸烟、灌服生理盐水;试验Ⅱ组吸烟、灌服普洱茶。饲养周期35d。试验Ⅰ组小鼠末体重和平均日增重显著低于(P<0.05)试验II组,试验II组低于(P<0.05)空白组。血红蛋白浓度和碳氧血红蛋白浓度,空白组和试验II组差异不显著,显著小于(P<0.05)试验I组;红细胞平均血红蛋白浓度,试验组Ⅰ和II差异不显著,显著高于(P<0.05)空白组。本试验结果证明,普洱茶可以显著改善被动吸烟小鼠生长性能和红细胞载氧功能,缓解烟毒危害。     

预处理对SFE提取月见草种子、被孢霉菌丝体油脂的影响

以月见草种子、被孢霉菌丝体为超临界流体萃取(SFE)的试验材料,研究了不同预处理条件(水分、粉碎度、粉碎方式)在不同萃取压力下对超临界流体萃取油脂得率的影响;确定粉碎度、粉碎方式、水分与油脂萃取之间的关系。     

甲醇柴油与生物柴油常规污染物的对比研究

通过对比实验研究了增压柴油机燃烧甲醇柴油和生物柴油对发动机动力性、经济性和排放特性的影响.研究结果表明:对于实验发动机,在喷油泵不做任何调整的情况下,直接燃烧甲醇柴油和生物柴油对柴油机动力性的影响均不超过10%.燃油甲醇柴油能降低CO和NOX的排放,但会引起HC排放量的上升;燃用生物柴油能降低CO和HC的排放,但会引起NOX排放量的上升.在各种工况下,燃用生物柴油均能大幅度降低柴油机的排气可见污染物,而燃用甲醇柴油在高负荷时会增加可见污染物的排放.     

工程造价的全过程管理

在工程建设中,只有把造价管理工作作为一项核心工作,才能真正降低工程造价,提高经济效益.本文对如何提高工程造价管理质量,如何加强工程造价的全过程管理的实施提出了几点建议.     

猪δ冠状病毒实时荧光RT-PCR检测方法的建立与应用

为灵敏、快速检测猪δ冠状病毒（porcine deltacoronavirus,PDCoV）,根据PDCoV M基因保守区域设计引物和探针,建立了一种PDCoV实时荧光RT-PCR检测方法,并评价了该方法的特异性、灵敏性和重复性。结果显示,该方法的拷贝数对数值与Ct值在1.3×103~1.3×107 copies/μL拷贝数浓度范围内呈现良好的线性关系,R2 = 0.994;最低检出限为13 copies/μL,且无非特异性扩增;批内和批间重复性试验变异系数（CV）为0.24%~2.15%,均小于3%。利用该方法和普通RT-PCR对42份临床样品同时进行检测,发现本方法比普通RT-PCR灵敏性更高,二者符合率为95.2%。以上结果表明,本试验建立的实时荧光RT-PCR方法灵敏、特异、可靠,可用于PDCoV的临床检测和流行病学调查。     

湖南小溪国家级自然保护区野生药用植物资源研究

对小溪国家级自然保护区的野生药用植物资源进行了初步调查。结果表明，小溪自然保护区有野生药用植物200科685属1420种。在介绍了88个科106种重要的药用植物药用部位和药用功能的基础上，就如何开发利用和保护药用植物资源提出了建议。     

广东乐从家具专业市场与家具产业链研究

广东省顺德市乐从家具专业市场2001年销售额为26亿多元,约占广东的1/3,而广东省家具市场销售占中国家具市场销售的80%。分析了乐从家具专业市场发展的三个阶段即马路市场、简易家具店铺群、家具会展经济。阐述了支撑乐从家具专业市场的巨大家具需求与供给,认为完美、成熟的家具产业链铸造了广东省林产工业的辉煌。