猪源ST9型耐甲氧西林金黄色葡萄球菌中前噬菌体的流行状况与转导分析

本研究旨在分析猪源ST9型耐甲氧西林金黄色葡萄球菌(Staphylococcus aureus,MRSA)中前噬菌体的流行情况、结构特点和转导能力,探究前噬菌体在猪源MRSA流行克隆形成中的作用。基于全基因组信息,分析了近年来从我国多省分离的131株ST9型MRSA中前噬菌体的流行率、分型、亲缘关系和结构特征;选取含不同分型前噬菌体的菌株进行诱导,对诱导获得的噬菌体颗粒进行转导,测定转导子的耐药表型及体外适应性。研究结果显示：猪源ST9型MRSA的前噬菌体携带率为78.6%(103/131株),其中,63株携带完整前噬菌体序列,所有前噬菌体序列均不含耐药基因,仅2.9%(3/103株)的前噬菌体序列含毒力基因;前噬菌体谱型丰富,其整合酶分型主要为Sa2int和Sa4int;各型别前噬菌体结构同源性较高,完整前噬菌体可被诱导为长尾噬菌体;噬菌体颗粒可包装供体菌的aadD、tet(L)耐药基因并转导至受体菌中;转导子可获得卡那霉素、四环素耐药表型,体外生长能力与受体菌株无明显差异(P>0.05)。研究结果表明：猪源ST9型MRSA的前噬菌体携带率较高,谱型丰富,不携带耐药基因,部分噬菌体可包装供体菌的耐药基因转导至受体菌,产生的适应性代价小。     

Simulation on the Characteristics of Flow Pattern in Sprial uP-flow Reactor

In order to analyze the reason why the Spiral Up-flow Reactor system has excellent removal effect of COD, N and P, the authors use PHOENICS, a computational fluid dynamics program, to investigate the hydraulic characteristics and flow model of the anaerobic part of Spiral uP-flow reactor. The result shows that the fluid in the reactor unit flowed upward by the spiral way, and that flow pattern was combined by plug flow and complete-mix flow, and that the percentage of plug flow was about sixty-five percent. Spiral Up-flow Reactor units the Characteristics of PFR and CSTR, which makes it work well.     

Test Analysis of Ecosystem on Functional Groups of Microorganism in Spiral Up- flow Reactor System

The test of functional groups of microorganisms in Spiral Up-flow Reactor System and the correlation between microorganisms in the water and the main environmental factors are studied.The results show that the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable.The close relations between abundance of bacteria and nutrients are found.The correlation between heterotrophic bacteria and COD is 0.949.The correlation between organic phosphate bacteria and TP is 0.815. The correlation between nitrosobacteria and NH_3-N is 0.909. The correlation between disnitrifier bacteria and TN is 0.653.     

东北刺人参种胚形态后熟过程中的解剖观察

通过对东北刺人参(Oplopanax elatus Nakai)种子后熟期间胚形态的解剖观察发现:东北刺人参果实成熟时种胚尚未分化完全,处于心形胚阶段,这是种子休眠的主要原因。种子在暖温处理过程中逐渐完成种胚的分化与生长,种子胚分化和发育的速度不一致,从而导致种子休眠程度不一致。     

玉米转Bt基因自交系的抗玉米螟特性鉴定初报

通过田间接卵和室内PCR分子检测,对采用回交法育成的Mo17Bt、478Bt、综3Bt和87-1Bt等4个转Bt基因自交系的抗虫性以及Bt基因的存在,进行鉴定.结果表明,4个转基因自交系群体的抗虫性明显提高,虫孔隧道长度比阴性对照平均缩短49.70%,差异达显著水平,但抗虫性不如阳性对照;群体内,单株间抗虫性仍存在差异,有高抗到高感不同类型,其     

Vehicle Lateral Stability Control Based on Sliding Model Control

In view of the vehicle cornering or path changing under ultimate handling maneuver, a 3-DOF nonlinear vehicle dynamics model which takes the vehicle lateral velocity, yaw rate and body roll angle as the State variables is presented. Based on the dynamic analysis, the method which using additional yaw moment produced by different longitudinal braking force among each wheels to improving vehicle handling stability in emergency situations is discussed. Considering the sideslip angle as one of the vehicle state variables is difficult to measuring, the sideslip angle estimation based on vehicle dynamic model and kinematics is designed. And then, based on the theory of siding model control, the combining sliding model control system is founded, which taking the vehicle yaw rate and body side-slip angle errors between the estimated and the real as the input variables, and taking the braking torque and the steering angle as control aims. The simulation results indicate that the control method can effectively improve the vehicle lateral handling stability.     

11种兰属植物DNA的提取及RAPD-PCR实验体系的建立与优化

采用改进的CTAB-DNA提取方法，从11种兰属植物的嫩叶中提取总DNA。所得的DNA样品的A260／A280值在1．7～1．9之间，琼脂糖凝胶上主带清晰，较少降解，样品纯度高，DNA量大。另外，对影响RAPD-PCR的Mg^2＋、dNTPs、Taq酶、引物浓度等因素进行了优化。确定优化的反应体系为：75ng模板DNA，1×Buffer，2．5mmol／LMg^2＋，0．15mmol／LdNTPs，0．75UTaq酶，引物浓度0．4μmol／L，反应总体积为25山。该体系在20个供试兰属实验材料中获得较好的扩增结果。     

感应电机矢量控制解耦算法的研究

矢量控制是通过坐标变换将感应电机模型转化为类似于直流电机的模型,使定子电流在同步旋转坐标系中的两个相互垂直分量分别独立控制转矩和转子磁链,从而获得类似于直流电机的调速性能。为此,对感应电机的数学模型进行了分析,通过矢量变换得到了在d、q坐标系下的闭环系统方程,对电机进行了解耦控制。在由TMS320F2812搭建的数字化实验平台上进行了实验,实验结果证明了研究方案的可行性。