放牧气候环境因素对泌乳母羊消化代谢的影响

试验选取6只内蒙古细毛羊健康泌乳母羊，分别于泌乳前期（Ⅰ、Ⅱ期）、泌乳中期（Ⅲ期）和泌乳后期（Ⅳ期），采用消化代谢、气体能量代谢等试验方法，运用灰色关联度分析法，探讨放牧条件下主要气候因素（气温、气湿、风速）对母羊不同泌乳期营养物质消化代谢的影响。结果表明，在本试验条件下，试验羊产热量受风速影响最大，机体能量存留量受气温影响最明显，而来食量、消化率、N存留量及总能代谢率等项指标与空气湿度关系最密切。     

植物无糖组培快繁装置及其环境控制系统的研制

针对常规植物组培存在的问题,从植物无糖组培实用化角度出发,研制了带有新型CO2施放装置的180 L植物无糖组培容器及其环境控制系统,采用小流量控制、三通阀调节和PWM控制方式,实现了对CO2浓度的精确控制,控制精度达到±50 μmol·mol^-1;采用穴盘覆膜与气体循环吸附相结合的方式实现了对容器内相对湿度的自动控制,控制精度达到±22%.通过圆叶海棠的无糖组培的试验,结果表明,该系统对组培苗的生长环境和生理品质的提高具有显著的促进作用.     

禽流感油乳佐剂疫苗质量快速检测方法的建立

通过对低温冻融法、研磨法、稀释高速捣碎法、95%乙醇法及三氯甲烷法破乳效果比较,确定三氯甲烷为油乳剂疫苗的破乳剂.将不同抗原含量的H9和H5亚型灭活与非灭活疫苗破乳后分离上层水相,采用红细胞凝集(HA)试验,测定其HA价,结果发现脱乳后上层水相与未做成油乳疫苗并做相应稀释的原液的HA价下降1～3个滴度,且将不同稀释度制备H5亚型疫苗经脱乳处理后上层水相的HA价与荧光定量PCR结果基本一致.所建立的禽流感油乳剂疫苗质量的检测方法具有快速、经济、简便,尤其适合于对大批量及基层应用.     

Prokaryotic Expression of Brucella BAB Gene and Establishment of iELISA

In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D_{450 nm} ≥ 0.607,it was judged as positive,when D_{450 nm} ≤ 0.561,it was judged as negative,and when 0.607<D_{450 nm}<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%.     

试卷分析系统的设计与应用

试卷分析是教学中不可缺少的重要环节,虽然简单,但繁琐、枯燥,特别是有的老师教了好几个班级,在班级人数又多的情况下,统计起来很费事,而且容易出错.用Visual Foxpro6.0编写试卷分析系统,并对该系统进行测试,测试结果显示该系统已经能够完成试卷分析的全部要求,分析的速度要比手工快5倍以上,准确率达100%,相信该系统会给各位老师带来方便.     

林业院校实践教学课程教师评学体系的探索研究

从构建合理的指标体系、健全组织体系、建立运行和反馈机制3个方面探索了如何构建实践教学的教师对学生评价体系。     

48kW拖拉机离合器设计与分析

进行了48 kW拖拉机离合器的设计并进行了结构改进:增加一套副离合器操纵系统,节省驾驶员的体力;开有梯形槽的碟形弹簧在制造过程中易产生应力集中,把梯形槽改为弧形槽使离合器寿命大有提高。     

甘德县草原使用权流转中的问题及对策

综合甘德县草原使用权流转的形式，分析了流转的原因、作用及存在的问题，并提出了甘德县草原使用权流转的对策建议。     

用厨余食物生物发酵饲料饲养肉鸡试验报告

将餐厅、宾馆、饭店、食堂等厨房加工菜肴的“边角余料”和餐桌的残余食物通过专用加工机械进行灭菌、生物发酵、干燥等工艺流程加工成饲料，作者将本饲料按不同配比添加在15～56日龄肉鸡饲料中，与常规饲料配方饲养进行对比试验。试验结果表明，试验组与对照组在肉鸡生长发育和鸡肉的品质等方面差异不显著，证明本饲料可以替代部分肉鸡浓缩料和玉米面，使厨余食物不但变废为宝，降低了饲养成本，还保护了环境。