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51.
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53.
Localised skin reactions (chancres) occurred on the flanks of cattle at the sites of deposition by tsetse flies of metacyclic forms of Trypanosoma congolense. Marked enlargement of the draining prefemoral lymph nodes accompanied the development of the skin reactions. Lymph from these nodes was collected through polyethylene cannulae inserted into the efferent lymphatics, and examined for trypanosomes, cells and antibody content. Within 6-9 days after infected tsetse fly bite, trypanosomes were detected in the efferent lymph; this preceded their appearance in the blood by 3-6 days, indicating that the lymphatic system acted as a major route for the passage of trypanosomes from the skin into the bloodstream. Responses induced in the draining lymph node as a result of trypanosome migration included a 2-3-fold increase in the volume of lymph and up to a 10-fold increase in lymphocyte output, including blast lymphocytes and plasma cells. Neutralising antibodies to metacyclic trypanosomes were detected in lymph and serum by Day 14 after infection, although in 2 out of 4 animals investigated, they were not demonstrated in serum until Day 18. Trypanosomes were also found in small numbers in efferent lymph of the prefemoral lymph node on the flank contralateral to the infected tsetse bites after development of parasitaemia. Increases in lymph flow and cellular output occurred about the same time in the ipsilateral and the contralateral efferent lymphatics, but were significantly less in the latter. Homologous challenge of immunised calves with tsetse-transmitted parasites revealed that trypanosomes were eliminated at the level of the skin or within the draining lymph node, as no parasites were detected in efferent lymph. 相似文献
54.
A simple trapping procedure for concentration of volatile organic compounds present in a head space and the subsequent analysis of the retained compounds by combined gas chromatographymass spectrometry is described. The procedure was used to study the organic metabolites evolved from various soils when they were amended with glucose and incubated under argon. A number of organic acids, alcohols, aldehydes and esters were evolved during a 4 week period. Less than 10 per cent of the added carbon was recovered as organic compounds, which was largely due to poor recovery of volatile organic acids from soil. Some implications of the observations to microbial ecology are discussed. 相似文献
55.
Confronting collinearity: comparing methods for disentangling the effects of habitat loss and fragmentation 总被引:1,自引:1,他引:0
Adam C. Smith Nicola Koper Charles M. Francis Lenore Fahrig 《Landscape Ecology》2009,24(10):1271-1285
Estimating the relative importance of habitat loss and fragmentation is necessary to estimate the potential benefits of specific
management actions and to ensure that limited conservation resources are used efficiently. However, estimating relative effects
is complicated because the two processes are highly correlated. Previous studies have used a wide variety of statistical methods
to separate their effects and we speculated that the published results may have been influenced by the methods used. We used
simulations to determine whether, under identical conditions, the following 7 methods generate different estimates of relative
importance for realistically correlated landscape predictors: residual regression, model or variable selection, averaged coefficients
from all supported models, summed Akaike weights, classical variance partitioning, hierarchical variance partitioning, and
a multiple regression model with no adjustments for collinearity. We found that different methods generated different rankings
of the predictors and that some metrics were strongly biased. Residual regression and variance partitioning were highly biased
by correlations among predictors and the bias depended on the direction of a predictor’s effect (positive vs. negative). Our
results suggest that many efforts to deal with the correlation between amount and fragmentation may have done more harm than
good. If confounding effects are controlled and adequate thought is given to the ecological mechanisms behind modeled predictors,
then standardized partial regression coefficients are unbiased estimates of the relative importance of amount and fragmentation,
even when predictors are highly correlated. 相似文献
56.
Abstract. A rotation trial of four years' pasture followed by two years' arable was used to study the effect of cropping on the morphological and hydraulic properties of soil. An adjacent paddock in grass for the past 35 years was included as a permanent pasture reference. Initial infiltration and field saturated hydraulic conductivity ( K fs ) were least for cultivated soil and increased with increasing time under pasture. This could be explained by the contrasting porosities of resin-impregnated blocks of undisturbed soil which had been infiltrated with methylene blue dye. Small K fs values for cultivated soil resulted mainly from a thin surface crust, although pore discontinuity at the depth of the cultivation pan (130 mm) could also have contributed. Greater K fs values under short-term pasture resulted primarily from water flowing through biogenic pores connected to the surface. The greatest K fs values were in soil that had been under pasture for 35 years (P35). This was attributed to flow through biogenic pores and fissures associated with the strongly-developed subangular blocky structure. The amount of water that infiltrated the two- and four-year pasture soils (P2 and P4) under ponding was 2.5 and 5 times greater, respectively, than the soil that had been cultivated for two years (C2).
As irrigation duration cannot be varied under the border-dyking system used on the Canterbury Plains, the interval between irrigations must be varied if the same total amount of water is to be applied to each of these soils through the season. The interval should be less for the cultivated soil than for those under pasture, and should increase with increasing time under pasture (i.e. P35 > P4 > P2 > C2). 相似文献
As irrigation duration cannot be varied under the border-dyking system used on the Canterbury Plains, the interval between irrigations must be varied if the same total amount of water is to be applied to each of these soils through the season. The interval should be less for the cultivated soil than for those under pasture, and should increase with increasing time under pasture (i.e. P35 > P4 > P2 > C2). 相似文献
57.
Prevalence of gastric lesions in foals without signs of gastric disease: an endoscopic survey 总被引:1,自引:0,他引:1
M J Murray C M Murray H J Sweeney J Weld N J Digby S J Stoneham 《Equine veterinary journal》1990,22(1):6-8
Gastroendoscopic examinations were conducted on 75 Thoroughbred foals aged two to 85 days on seven breeding farms in England and Ireland. The foals showed no signs of gastric disease. There was no significant difference between lesion prevalence in foals in England (16 of 28 foals; 57 per cent) or Ireland (22 of 47 foals; 47 per cent). Neither was there any sex predilection (18 of 36 males; 20 of 39 females). Lesions were most prevalent in foals under 10 days old (8 of 9) and least prevalent in foals older than 70 days (3 of 10). Lesions occurred most frequently in the squamous mucosa immediately adjacent to the margo plicatus along the greater curvature (34 foals), whereas lesions in the squamous fundus, the glandular fundus, and the lesser curvature were observed in 11, six and three foals, respectively. Lesions were not observed in the squamous mucosa surrounding the cardia. Gastric lesions were more prevalent in foals with a previous disorder than in those without (68 vs 43 per cent) and particularly with a history of recent or concurrent diarrhoea (9 of 14). 相似文献
58.
Marron N Dreyer E Boudouresque E Delay D Petit JM Delmotte FM Brignolas F 《Tree physiology》2003,23(18):1225-1235
Responses to successive drought and re-watering cycles (1-3 cycles) were compared in greenhouse-grown cuttings of Populus x canadensis (Moench) clones, 'Luisa_ Avanzo' and 'Dorskamp.' Total leaf number increment rate, duration of leaf expansion, total and individual leaf area expansion rates and stomatal conductance were recorded periodically during the experiment. Soil water content (SWC) and predawn leaf water potential (Psi(WP)) were measured four times during each drought cycle. In parallel, relative leaf water content (RWC) and specific leaf area (SLA) were estimated on leaves collected from the top to bottom of each cutting. Under well-watered conditions, 'Luisa_Avanzo' and 'Dorskamp' differed in their patterns of leaf area expansion. Although duration of leaf expansion was similar between clones, 'Luisa_ Avanzo' exhibited higher total leaf number increment rates and individual leaf area increases than 'Dorskamp.' As a result, 'Luisa_Avanzo' cuttings reached larger individual and total leaf areas than 'Dorskamp.' 'Dorskamp' leaves had lower SLA than 'Luisa_Avanzo' leaves. In response to successive drought cycles, both clones underwent decreases in total leaf number increment rates and in total leaf area expansion rates, but both whole-plant and individual leaf areas were drastically reduced only in 'Luisa_Avanzo.' 'Dorskamp' maintained a constant leaf area as a result of an increase in the duration of leaf expansion during drought and a significant stimulation of individual leaf area expansion rate and total leaf number increment rate in response to re-watering. Drought caused a greater decrease in SLA in 'Luisa_Avanzo' than in 'Dorskamp.' Expanded leaves of 'Dorskamp' were constitutively dense or thick, or both, whereas leaves of 'Luisa_Avanzo' became dense or thick, or both, only in response to drought. In both clones, re-watering caused partial recovery of SLA to control values. Our data confirm previous field and greenhouse observations that 'Dorskamp' is more tolerant to successive drought and re-watering cycles than 'Luisa_Avanzo.' We conclude that this difference is mainly attributable to the plasticity of 'Dorskamp' after re-watering, rather than to clonal differences in drought responses. 相似文献
59.
Summary In view of the known orientation of microfibrils about pits in conifer tracheids a model is presented which is consistent with the assumption that this pattern derives from the slow viscous flow of a fluid about a cylindrical obstacle.At present at the Botany Department, La Trobe University, Bundoora, 3083, Victoria, AustraliaThe authors wish to thank Dr. W. B. Thompson and Professor A. B. Wardrop for their constructive criticism. 相似文献
60.
We followed the establishment and growth response of 13 commercial tree species to canopy opening, above-ground biomass removal, and experimental burns of low and high intensities in a lowland dry forest in Bolivia. Three patterns of response to treatments were observed among the most abundant commercial tree species. (1) Shade-intolerant species regenerated mostly from seed and had the highest survival and growth rates following high-intensity burns. (2) Shade-tolerant species were abundant in gap control and plant removal treatments. Treatments had little effect on the height growth of these species. (3) Individuals of root sprouting species were most abundant following plant removal and low-intensity burn treatments. Treatments had little effect on the height growth of these species. The wide variation in species responses to gap treatments found in this study not only reinforces the concept that species are distributed along a continuum of shade-tolerance levels, but that other aspects of species biology, such as seed dispersal type or sprouting behavior, further differentiate regeneration strategies. The variety of regeneration strategies found among the species at this forest site will require a flexible management scheme that mixes more intensive silvicultural treatments such as prescribed burning with less intensive treatments. 相似文献