与菜用大豆收割机配套的种植密度筛选试验

对2个菜用大豆品种毛豆3号、浙鲜豆8号进行了适用收割机采收的种植密度试验,分别比较了植株性状、豆荚和产量构成性状、产量。试验结果表明,适宜于毛豆3号、浙鲜豆8号收割机采收的种植密度为4100~4400穴/667 m~2。     

慈竹生长过程纤维素、木质素及灰分动态积累

以当年生不同生长高度的慈竹为研究对象,探讨慈竹生长过程中纤维素、木质素和灰分含量的积累规律。结果表明:慈竹不同生长高度纤维素含量顶、中部变化趋势均以对数方式增长;基部纤维素含量和茎秆纤维素总含量变化趋势都呈"S型"曲线;各部位木质素含量变化趋势总体呈倒"S型"曲线,且不同高度间的木质素含量的差异达显著水平;随着生长高度的增高,茎秆顶部、基部灰分含量以及茎秆灰分总含量总体呈倒"S型"曲线变化,中部灰分含量总体呈"S型"曲线变化。纤维素总含量、木质素总含量的积累分别集中在茎秆高度为150~800、30~800 cm这个生长时期;灰分总量在300~800 cm保持较高的水平。     

Determination and Analysis of Heavy Metal Content in the Soil from Beef Cattle Farms in Three Regions of Xinjiang

In order to study and evaluate the feeding environment of the beef cattle farms in Xinjiang, the contents of heavy mentals in the soil from 8 beef cattle farms in Xinjiang Aksu, Yili and Tacheng regions were tested according to the Field of Livestock and Poultry Environment Quality Standard (NY/T 388—1999).The atomic absorption spectroscopic method was used to determine the contents of As, Hg, Pb, Cd, and Cr.The mean contents of As, Hg, Pb, Cd, and Cr in 8 beef cattle farms soil were 0.389, 0.568, 22.708, 0.573 and 149.449 mg/kg, respectively, and they were all below the upper limit of NY/T 388—1999.The comprehensive heavy metal pollution index of 8 beef cattle farms were 0.577 to 0.994, and the index were 0.7 to 1 of two observation points in Yili and one observation point in Tacheng.The soil environment quality of these 3 farms were at the level of slight cleaning.The comprehensive heavy metal pollution index of other five observation points were ≤0.7, the soil environment was in a clean level.The soil environment of beef cattle farms in these three regions conformed to the standards of livestock for environment quality and safe products.     

我国管理科学研究热点及代表人物的可视化分析

进入新世纪以来，管理科学在我国取得了蓬勃的发展，逐渐形成了庞杂的学科知识体系，与此同时，管理学也涌现了一大批管理科学专家，并在各自的领域中作出了卓越的贡献。利用中国期刊全文数据库（CNKI）2000~2009年的20种管理科学重要期刊文献为数据，从文献计量的角度对我国管理科学十年来的发展进行分析，通过可视化分析工具CiteSpace展示了企业与事业单位管理、管理基础与方法、宏观管理与政策研究三个主要的学科领域的代表人物和研究热点。     

贵州息烽天台山黄心夜合种群结构和动态研究初报

在以黄心夜合(Michelia martinii)为优势种群的森林群落内设置1200 m2样地,用相邻格子法进行每木调查获取野外资料.通过对种群大小级(立木级)数量特征和分布格局分析,探讨了黄心夜合种群结构和不同发育阶段种群格局动态.结果表明,黄心夜合种群结构呈不完整的金字塔型,中小径级种群缺失较多,但有一定数量的幼苗幼树(Ⅰ级和Ⅱ级),仍属于稳定型种群或进入成熟型阶段.种群不同发育阶段分布格局由集群分布向随机分布转变,集群强度由强变弱,种群密度由大变小,群落仍处于相对稳定阶段.     

日光温室蔬菜栽培废弃基质露地还田对土壤理化性质及辣椒生理特性和产量的影响

为了研究基质还田对大田土壤和辣椒生长生产的影响,以日光温室连续栽培蔬菜6年的废弃基质还施露地种植辣椒,测定了不同施用量(0,45,90,135 m~3/hm~2)的土壤理化性质及辣椒生理特性和产量。结果表明:基质还田能够减少土壤pH变化,减小土壤容重、略微增加土壤孔隙度、保持土壤气水比,改善了土壤的理化性状,促进辣椒植株的生长且利于其干物质积累,其中株高、茎粗及干物质积累量均以T2最好。提升了辣椒叶片光合荧光的表现,T2的净光合速率、蒸腾速率、气孔导度、Fv/Fm、ΦPSⅡ均为最高,T1的q P最高,NPQ为T3最低。提高了辣椒果实的品质及产量,T2产量达67.04 t/hm~2,可溶性糖含量以T2最高,为2.21%,Vc含量T3最高,为1.97 mg/g,T1的可溶性蛋白含量最高、硝酸盐含量最低,分别为2.07,204.93 mg/kg。而与此同时,较大的还田施用量(135 m~3/hm~2)在辣椒生长的中前期造成了一定的毒害现象,在生育后期此现象消失,最终各项指标全面优于未还田处理。废弃基质露地还田这样的重复利用方式切实有效,(在种植同科作物的情况下)还田施用量宜选择90 m~3/hm~2。     

丁酸钠对犊牛胃肠道发育的研究进展

丁酸钠是一种绿色新型饲料添加剂，目前在动物饲料中应用广泛，且效果良好。作者主要介绍了丁酸钠的生物学功能、对犊牛胃肠道发育的影响及其在犊牛日粮中的应用效果。重点总结了丁酸钠促进犊牛胃肠道发育的影响机制，包括作为胃肠道组织的能量来源调控激素和生长因子的分泌，提高消化和吸收相关酶的分泌及活性，提高肠道免疫细胞保护性蛋白的分泌等。现有研究表明，日粮干物质中添加0.3%丁酸钠就能达到促进犊牛生长和胃肠道发育的效果，但具体添加水平、添加阶段和方式等尚不明确。此外，日粮粗饲料水平、精料发酵所产生的内源丁酸对外源丁酸钠的作用效果是否有影响也鲜有报道，有待进一步开展研究。     

Celecoxib inhibits viability,induces apoptosis and inhibits autophagy in acute myeloid leukemia cell lines HL-60 and HL-60A

AIM: To investigate the effects of celecoxib on viability, apoptosis and autophagy in acute myeloid leukemia (AML) cell lines HL-60 and HL-60A. METHODS: The HL-60 cells and HL-60A cells were cultured with various concentrations (0, 20, 40, 60, 80 and 100 μmol/L) of celecoxib. The inhibitory effect of celecoxib on the cell viability was evaluated by MTT assay. Apoptosis was analyzed by Annexin-V/PI staining. Apoptosis-related and autophagy-related proteins were determined by Western blot. RESULTS: IC₅₀ of celecoxib were 49.4 μmol/L, 32.0 μmol/L and 25.1 μmol/L for HL-60 cells treated with celecoxib for 24 h, 48 h and 72 h, respectively. For HL-60A cells, the corresponding IC₅₀ were 69.1 μmol/L, 42.5 μmol/L and 29.6 μmol/L, respectively. The results of flow cytometry analysis showed the proportions of Annexin-Ⅴ⁺ PI^-, Annexin-Ⅴ⁺ PI⁺ and Annexin-Ⅴ^-PI⁺ cells were increased in the HL-60 cells, and those of Annexin-Ⅴ⁺PI^- and Annexin-Ⅴ⁺ PI⁺ cells were increased in the HL-60A cells treated with celecoxib for 24 h. After treated with celecoxib, the induction of apoptosis was observed, the apoptosis-related proteins cleaved caspase-3 and cleaved PARP were upregulated, the autophagy-related proteins LC3 II and P62 were both increased, and mTOR, p-mTOR, 4-EBP and p-4-EBP were not changed, indicating that celecoxib inhibited autophagy in the AML cells without the mTOR pathway involvement. CONCLUSION: Celecoxib inhibits the viability of HL-60 cells and HL-60A cells in a time-and dose-dependent manner by its effects of inducing apoptosis and necrosis. Celecoxib inhibits mTOR-independent autophagy in AML cells, indicating a possible way of using celecoxib for enhancing the antitumor activity of therapeutic agents to induce cytoprotective autophagy in the AML cells.     

华北驼绒藜雨季移栽成活率研究

本文是不同季节移栽华北驼绒藜(Ceratoides arborescens)系列研究之一。干旱区春季生态建植有补水之苦,雨季移栽表明,不需坐水或补水,成活率可高达60%,浸根可提高成活率至90%;说明雨季移栽是可行的。移栽植株数日内叶片含水量和叶绿素含量急剧下降、脯氨酸含量骤然升高,含量恢复过程则较缓慢,说明移栽使植株突然损伤,但终能恢复至正常生理状态。     

甘肃省农村信息化发展水平探讨

农村信息化是社会主义新农村建设的重要内容,是解决"三农"问题的突破口。对于欠发达的甘肃农村经济,近年来虽然取得很大进步,但总体水平还不高,与发达地区的差距还很明显。在客观数据的基础上,基于因子分析方法建立了综合评价农村信息化水平的模型,直观的比较了各省市的农村信息化水平,认为信息资源的利用情况和信息基础设施建设的差异是评价农村信息化水平的决定因素,提高农村信息化水平必须从这两方面着手,切实可行的推进甘肃农村信息化的发展。