奶牛秸秆型日粮矿物质营养水平评价

对淄博地区奶牛营养两次调查过程中,发现秸杆型日粮饲喂的奶牛可能存在矿物质缺乏问题。针对这一问题,测定日粮中钙、磷、铜、锰、铁、锌和奶牛血液中铜、锰、铁、锌元素的浓度,用以评价秸杆型日粮奶牛矿物质营养水平。结果表明:①秸杆型日粮存在矿物质营养不平衡问题,锰、锌元素不足,铁元素过剩。②秸杆型日粮矿物质营养具有互补作用。③评价奶牛矿物质营养水平的手段有待改进。     

野生植物白沙蒿资源的综合开发利用研究

对白沙蒿的生物学特性、化学成分及开发应用进行了综合研究，结果表明：白沙蒿是理想的抗旱、防沙和固沙植物；含有丰富的营养物质，可作为畜牧业的重要饲料；有较高的药用价值和工业利用前景，对该植物资源的开发利用提供了科学依据。     

人工林UHF频段电波传播场强预测模型

人工林无线电波传播场强模型是合理设计人工林环境无线电遥测遥控等通信电路的理论依据.该文在分析无线电波传播机理的基础上确定了人工林UHF频段传播损耗的三大组成部分及相应的计算方法,提出了人工林中某接收点处电场强度的预测模型.经过对北京西山人工侧柏林中不同参数的场强测试数据进行分析,校验了所建模型具有很高的精度.      

白菜叶和茎中微量元素Cu、Mn含量的测定

为研究白菜的食品应用及工业应用价值,采用HNO3-HCl O4(4∶1)湿法消解样品,火焰原子吸收光谱法(FAAS)测定白菜叶和茎中微量元素Cu、Mn的含量。试验结果表明,白菜叶和茎中Mn、Cu含量存在明显差异,Mn的含量均大于Cu的含量,且这两种元素在叶中的含量均高于茎。     

东北苹果产区抗寒砧木研究与利用进展

该文针对我国东北苹果栽培区域的不同气候特点,总结了各地栽培现状,在综合前人研究成果的基础上,概述了不同气候区的苹果主栽品种和主要砧穗组合;对苹果主要砧木资源、抗寒性研究方法及应用现状进行综述,并对抗寒砧木的利用提出建议。     

Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation,migration and invasion

AIM: To compare the expression of SIRT2 in ovarian surface epithelial (OSE) cell line and serous ovarian carcinoma (SOC) cell lines, and to investigate the effects of SIRT2 on the cell proliferation, migration and invasion. METHODS: The expression levels of SIRT2 in the OSE cell line and the SOC cell lines were determined by Western blot. The SIRT2 siRNAs and overexpression construct were designed and verified. Transient transfection of SIRT2 siRNAs or overexpression construct was performed, and the effect of SIRT2 on the cell proliferation, migration and invasion was evaluated. RESULTS: SIRT2 levels in the 5 strains of SOC cell lines were significantly lower than that in the OSE cell line. SIRT2 knockdown in HOSEpiC cells significantly enhanced the ability of cell colony formation and accelerated the cell growth rate. On the contrary, overexpression of SIRT2 in HO8910 cells dramatically repressed the number of cell colonies and cell activity. SIRT2 significantly changed the ability of ovarian cell migration. Knockdown of SIRT2 facilitated the cell invasion. CONCLUSION: The expression of SIRT2 in the SOC cells is significantly down-regulated. In the OSE cells, SIRT2 acts as a tumor suppressor and mediates the inhibition of cell proliferation, migration and invasion.     

Mesenchymal stem cell-induced CD8α+ Jagged2high CD11bhigh regulatory dendritic cells prevent and treat mouse aGVHD

AIM: To investigate the role of mesenchymal stem cell-induced regulatory dendritic cells (MSC-DCregs) in mouse acute graft-versus-host disease(aGVHD) model. METHODS: Bone marrow cells from BALB/c (H-2^d) mice were isolated and were induced to differentiate into DCs. The DCs were selected by flow cytometry, and after 10 d co-culture with MSCs, they were induced to be MSC-DCregs. Male 8-week-old C57BL/6 (H-2^b) mice were used as donor mice. The female 8-week-old BALB/c (H-2^d) mice, who had received 100 cm source-skin distance, 30 cm×30 cm radiation field, 700 cGy total body irradiation (TBI) pretreatment were used as recipient mice. The recipients were divided into 5 groups: control group, TBI group (injected with medium only), bone marrow transplantation group (injected with 1×10⁷ bone marrow cells), aGVHD group (injected with 1×10⁷ bone marrow cells and 1×10⁷ spleen cells), and MSC-DCregs group (injected with 1×10⁷ bone marrow cells, 1×10⁷ spleen cells and 1×10⁶ MSC-DCregs). The white blood cell count, recipients' chimerism, clinical evaluation of aGVHD, survival analysis and pathological changes were determined. RESULTS: Hematopoieic recovery was seen at 10 d after transplantation. The recipients' chimerism was parallel to the donors' at 30 d. The median survival time of the mice in aGVHD group and MSC-DCregs group was 27 d and 33 d, and the survival rates at 30 d were 20% and 100% (P<0.01), respectively. The clinical scores of the mice in MSC-DCregs group were lower than those in aGVHD group (P<0.01). Moreover, the pathological changes in the skin and liver of the mice in MSC-DCregs group were less serious than those in aGVHD group. CONCLUSION: The MSC-DCregs induce an aGVHD tolerance in vivo, and further research of its mechanism is still in great necessary.     

番茄对晚疫病的株龄相关抗性及Ph-3的抗性机制

目前已明确番茄抗晚疫病R基因表现明显的株龄相关抗性(Age-related Resistance,ARR),但抗晚疫病QTL的抗性规律尚不明确。以携带抗晚疫病基因Ph-3的栽培种番茄(Solanum lycopersicum)‘CLN2037B’和野生种醋栗番茄(S.pimpinellifolium)‘L3708’以及易感病材料栽培种番茄(S.lycopersicum)‘LA2818’为对照,对含有抗晚疫病QTL的多毛番茄(S.habrochaites)‘LA2099’、‘LA1033’和‘LA1777’是否也存在株龄相关抗性进行了分析。结果表明,携带抗晚疫病QTL的3个材料的6叶期和9叶期植株病情级数均比3叶期明显降低,表明QTL抗性与株龄相关。利用乙烯、水杨酸和茉莉酸素合成或缺失的突变体和病毒诱导基因沉默(Virus Induced Gene Silencing,VIGS)技术,初步明确了乙烯和水杨酸参与6叶期Ph-3基因介导的对晚疫病的抗性,而茉莉酸不参与。     

不同栽培方式对长江下游棉田资源利用效率的影响

我国农业对自然环境依赖性强,农业生产环境相对恶劣、资源利用效率低下,作物栽培理论与技术需要不断创新和完善。为建立与当前生产模式相匹配的作物高产高效栽培管理方式,选用棉花品种泗杂3号,于2012—2013年在长江下游棉区(江苏大丰)不同地力水平田块(高、低)进行麦棉两熟栽培管理方式定位试验,设超高产栽培、常规栽培和高产高效栽培,系统测定棉花生物量、产量和生育期间的温光、氮肥资源利用效率。结果表明,栽培方式和地力水平显著影响棉花产量,而产量的差异主要由温光、氮肥资源利用效率的差异造成。棉花产量提高的限制因子是低下的资源利用效率。高产高效栽培较常规栽培产量提高27.5%,温光资源利用效率分别提高27.7%、23.4%、氮肥偏生产力提高10.1%,是长江下游较为适宜的栽培方式。因此未来生产中应进一步合理优化栽培方式来提高棉田资源利用效率,以达到高产高效的目标。     

春小麦微量营养元素叶面喷施的效应研究Ⅱ——对后期NAR,LAR,RGR,CGR及生物产量的影响

选用微量及大量营养元素11个在春小麦抽穗后叶面喷施,研究其对重要生理指标NAR,LAR,RGR,CGR及生物产量的影响.结果表明各处理对NAR影响较大,以P最高,Se次之,而Fe,Cu,B,Mn,K均低于对照;对LAR影响不大,仅Fe高于对照;对RGR的影响Zn,Cu,Fe,Se,Mn,Mg,Mo,P,N,K均超过对照,并依次增高,表明这些营养元素均可提高相对生长率RGR,其作用以K,N,P居前3位;对CGR的影响,各处理有较大差异,除B处理和对照相近外,其他均高于对照,以K,N,Mo,P,Mg居前列,表明这些营养元素,对后期干物质增长有利.各处理对增加生物产量有益,且各不相同,其效应K＞N＞Mo＞P＞Mg＞Se＞Mn＞Ca＞Fe＞Zn＞B＞ck.