Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.     

高产、抗病转基因抗虫棉品种——中棉所94A915

概述了中棉所94A915的选育经过、特征特性，并总结了其关键栽培技术。     

基于UHPLC-Q-TOF/MS的不同产地普洱生茶化学成分差异研究

为了更加全面地了解产地对普洱生茶品质与化学成分的影响,本研究选取来自临沧市、普洱市、西双版纳州三大产区12个茶山(自然村)的普洱生茶样品,采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q-TOF/MS)对不同产地普洱生茶的非挥发性代谢物表型进行了研究。结果表明:不同产地普洱生茶的化学成分在含量上具有较大差异,且具有明显的产地特征。采用主成分分析,可以对来自西双版纳自治州(包括勐腊县、勐海县、景洪市)、普洱市、临沧市3个地级行政区的普洱生茶进行有效区分,也可以对来自普洱茶产区的东南、西南、西北3个区域的普洱生茶进行有效区分。进一步鉴定了普洱生茶中79种主要成分,并对其在12个茶山(自然村)的普洱生茶中的含量分布,以及与不同产地普洱生茶滋味品质的关系进行了分析。本研究表明,基于UHPLC-Q-TOF/MS的茶叶非挥发性化学成分轮廓可以作为普洱生茶产地判别的依据。     

单胚甜橙优系—王字4号的生物学特性及经济性状研究

王字４号是四川新胜茶场选出的甜橙（ＣｉｔｒｕｓＳｉｎｅｎｓｉｓ）优良单株，具果大、质优、少核、晚熟、较耐低温等综合优良性状。特别是单胚率高而稳定，１９９０—１９９２年镜检，单胚率分别为９８．１５％、９５．６％、９７．７％。     

红桔果实粒化机理与控制途径的研究

本文根据果皮组织、果实比重、呼吸强度和乙醇、果胶甲酯酶等的变化,对红桔贮藏中的粒化机理进行了探讨。用植物激素和杀菌剂组成的ZCPⅢ复合保鲜剂作采后浸果处理,对浮皮、粒化和腐烂的防效可达90%以上。     

河北省桑属植物染色体倍数性研究

研究了桑属植物５个种３２个品种的染色体倍数性，发现宽城１０号等９个品种为三倍体（２ｎ＝３ｘ＝４２），其余为二倍体（２ｎ＝２ｘ＝２８）。     

谏壁发电厂泵站改造实践

为解决渣浆泵在运行时轴封水供应困难的问题，计算了谏壁发电厂松林山泵站的装置特必,天详细分析论证的基础上，确定了泵站的改造方案，进行了叶轮的优化设计。     

草甘膦在茶园中的应用研究

草甘膦是一种具有内吸传导、杀草谱广、毒性低等特性的新型化学除草剂,对根除茶园常见杂草有特效。用草甘膦防除茶园杂草的最佳浓度因杂草种类不同而异,以一、二年生杂草为主的茶园用0.10％的浓度为宜,以多年生杂草为主的茶园用0.15％的浓度较好。用液量以杂草充分湿润为度。全年喷药一次或二次即可,若喷一次,宜在五月下旬进行;若秋季草害较重,则在八月下旬复喷一次。使用该方法除草,全年只需耗药费4.80元～6.40/亩,除草效果优于全年人工除草三次者,节省除草工7～9个/亩,增产5～7％,对保持茶园生态平衡,提高茶叶品质均有益。     

Alternative complement pathway in bovine serum: lysis of human erythrocytes.

The hemolysis of unsensitized human erythrocytes by fresh bovine serums was investigated. Lysis occurred in ethylene glycol bis-amino tetraacetate buffers and with serums depleted of Clq. Serums extensively absorbed with packed human erythrocytes at 0 C effectively lysed human erythrocytes, but optimal lytic capacity required target cells "sensitized" with a heat-stable serum factor. Lysis did not occur with serums absorbed with zymosan at 17 C or heat inactivated at 50 C. These results indicate that human erythrocytes can activate the alternative pathway of complement in bovine serums. Lysis can proceed in the apparent absence of antibodies, although their presence may enhance the reaction.     

盐胁迫对黄瓜幼苗渗透调节物质含量的影响

土壤盐渍化制约作物生育，同时也是作物品质劣变的主要因素之一。近年来由于环境保护措施的滞后，不科学的施肥及土壤管理，导致土壤次生盐渍化现象越来越严重。渗透调节物质在细胞中积累能够提高细胞渗透调节能力，稳定其内大分子蛋白生物膜的结构和功能。研究盐胁迫下渗透调节物质含量的变化规律可以了解其与耐盐性的相关性。本试验研究了盐胁迫对黄瓜幼苗几种渗透调节物质含量的影响，以期为黄瓜耐盐育种提供理论依据。