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721.
Brenda S. Phillips Marcia L. Padilla Erin B. Dickerson Mary J. Lindstrom Stuart C. Helfand 《Veterinary immunology and immunopathology》1999,70(3-4):189-201
Interleukin-12 (IL-12) plays a pivotal role in regulating cellular immune responses involving autoimmunity, infectious disease, and cancer. Human recombinant (hr) IL-12 is being evaluated for therapy of human cancer. We investigated the potential of hrIL-12 to activate canine peripheral blood mononuclear cells (PBMC) using proliferation and cytotoxicity as readouts. Human rIL-12 caused increased proliferation of PBMC, and enhanced lysis of allogeneic canine tumor targets mediated by PBMC from normal dogs in vitro. In addition, antibody-dependent cellular cytotoxicity (ADCC) mediated by canine PBMC was enhanced by hrIL-12. These results indicate that hrIL-12 is recognized by canine immune cells, triggering a number of immune responses in canine PBMC, that may be important for immunotherapy of canine cancer. Information from this investigation provides impetus for evaluation of the effects of hrIL-12 on PBMC from tumor-bearing dogs and should be helpful in the development of hrIL-12 as an immune cell activator in vivo in the dog. 相似文献
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723.
Paolo Guidetti Piero Addis Fabrizio Atzori Simona Bussotti Antonio Cal Alessandro Cau Jean‐Michel Culioli Giuseppe De Lucia Antonio Di Franco Manfredi Di Lorenzo Maria Cristina Follesa Vittorio Gazale Giorgio Massaro Francesco Mura Augusto Navone David Pala Pier Augusto Panzalis Antonio Pusceddu Alberto Ruiu Angelo Cau 《水产资源保护:海洋与淡水生态系统》2019,29(4):537-545
724.
Maritza Leonardi Jeanett Vera Eduardo Tarifeño Marcia Puchi Violeta Morín 《Fish physiology and biochemistry》2010,36(3):757-765
We sought to provide a useful indicator of the presence of endocrine-disrupting contaminants along the marine coast of the
South Pacific using Chilean flounder (Paralichthys adspersus). In light of the lack of information on vitellogenin for this species, we induced, purified, and identified the plasma vitellogenin
of Chilean flounder inhabiting the Chilean coast. Vitellogenin (Vg) from Chilean flounder was purified by size exclusion and
ion-exchange chromatography using plasma from juvenile males induced by injecting 17β-estradiol. The Vg was detected by SDS–PAGE and Western blot analyses using an antibody against turbot (Scophthalmus maximus) vitellogenin. These analyses revealed a protein band of 205 kDa and three minor bands of 120, 90, and 68 kDa. These proteins
were identified as Vg by means of mass spectrometry (LCQ Duo ESI-IT-MS), matching sequences of tryptic peptides to known sequences
for several other fish species. The matches showed the presence of vitellogenin (VgI, VgII, Vg A and Vg B) in Chilean flounder,
similar to species such as mummichog (Fundulus heteroclitus), Japanese medaka (Oryzias latipes), and white perch (Morone americana). These results are discussed in terms of identifying Vg in Paralichthys adspersus with the antibody to turbot Vg. Moreover, we compare the molecular size of Vg from Chilean flounder (large) with that of
other flatfish species. Finally, we discuss the potential use of this molecule as a biomarker for the presence of xeno-estrogenic
compounds along the Chilean coastline. 相似文献
725.
Fish Physiology and Biochemistry - The presence of perfluoroalkyl substances (PFASs) in the environment, especially in aquatic ecosystems, continues to be a significant concern for human and... 相似文献
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727.
Marcia Sayuri Iquematsu Eduardo Ribeiro Cunha Andréa Bialetzki 《Ecology of Freshwater Fish》2023,32(1):120-132
Structural habitat complexity provided by macrophytes is expected to increase richness and abundance of fish species. However, this topic is rarely investigated simultaneously at different periods of fish development. We sampled fish within macrophyte stands and in non-vegetated areas at floodplain lakes and tested the hypothesis that the presence of macrophytes increases abundance and species richness of fish, in addition to changes in species composition, at different periods of fish development. Our findings demonstrated that, in different period of fish development, the highest values of fish species richness and abundance were found at sites colonised by macrophytes. Similarly, changes in fish species composition were observed between habitats colonised by macrophytes and non-vegetated areas. Therefore, the results demonstrate that macrophyte presence plays an important role in regulating fish community structure at different periods of fish development. 相似文献