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  1. In the Recent, brachiopods only seldom occur in benthic communities. A biotope dominated by Megerlia truncata was identified in 2013 by exploration of the south‐easternmost Adriatic margin by Remotely Operated Vehicle (ROV).
  2. Emerging rocky substrates next to the shelf break at approximately 120 m appear intensively exploited by this eurybathic rhynchonelliformean brachiopod, attaining a population of more than 300 individuals per m2.
  3. Calcareous red algae are almost ubiquitous at this site and preferentially encrust sectors of the substrate where brachiopods are minimal.
  4. This Megerlia biotope is a novel finding for this part of the Mediterranean Sea, similar to a situation previously observed in the Western Basin, off the Mediterranean French coast.
  5. It is proposed that this remarkable brachiopod biotope and the adjacent rhodolith bed are considered important for conservation management.
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  1. Association between habitat structuring organisms and other species has great relevance for ecosystem‐based conservation measures.
  2. Those occurring in temperate areas, particularly in the upper portion of the continental margin, are mostly unknown or not properly understood because of the difficulty to discriminate co‐occurrence and real functional linkages among species.
  3. Deep water coral assemblages over the Carloforte Shoal (south‐west Sardinia; 180–210 m depth) were investigated using ROV surveys.
  4. During the surveys, more than 740 egg‐capsules of the spotted catshark Scyliorhinus canicula, identified after hatching experiments in captivity, were found attached exclusively to colonies of the long‐living black coral Leiopathes glaberrima.
  5. Although based on a spot finding, the results show that coral forests are not only hotspots of biodiversity, but can also serve as nursery grounds for S. canicula. The protection of these millennial coral forests is therefore to be considered a priority.
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  1. Cold‐water coral (CWC) ecosystems are protected both in national and international waters. They are considered Vulnerable Marine Ecosystems and, in addition, meet the criteria to be defined ecological or biological significant areas.
  2. Two new sites of the “Sardinian CWC province” were discovered by means of remotely operated vehicle surveys in two submarine canyons in the north‐eastern portion of the Sardinian continental margin, at depths ranging from 230 to 430 m.
  3. Dense and healthy patches of small and medium‐sized Madrepora oculata colonies were found dwelling on rocky walls and inclined silted soft bottoms.
  4. A rich and diverse associated megafauna was found within both live and dead portions of the coral framework. In addition, the presence of benthic litter and derelict fishing gears were also documented.
  5. These results further extend the “Sardinian CWC province” extension, providing insights of a potential CWC network along the Sardinian continental margin with implications for the Mediterranean mosaic of CWC provinces, further stressing the need for urgent conservation measures for these habitats.
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Biotinylated lectins and avidin-biotin-peroxidase complex were used to study the correlation between cellular glycoconjugates' expression and squamous maturation in normal canine skin and in various epithelial neoplasms. Normal skin tissue was obtained from five, male, random-source dogs, 5 to 7 years old. The tumors tested, selected from the files of our Department, were fifteen squamous cell carcinomas from different tissue origin, five hepatoid perianal gland adenocarcinomas with squamous metaplasia, and fourteen solid mammary carcinomas with and without histologic evidence of squamous metaplasia. Except for mammary gland carcinomas, all tumors had been surgically excised from male dogs. Intermediate filament aggregation of twelve solid mammary gland carcinomas were studied electron microscopically. The basal and the lower spinous cells in normal skin and the less differentiated cells in squamous cell carcinomas stained moderately with Griffonia simplicifolia agglutinin-I. Spinous and granular cell layers stained strongly with Phytolacca americana mitogen and Arachis hypogaea agglutinin. Both lectins stained well-differentiated cells in squamous cell carcinomas. The electron microscopic study carried out in solid carcinomas of mammary glands revealed some relationship between the presence of intracytoplasmic tonofibrils and the binding of Griffonia simplicifolia agglutinin-I and Phytolacca americana mitogen to the tumors tested. Our results suggest that the glycosylation pattern occurring during normal keratinocyte differentiation is conserved in squamous cell carcinomas and that Griffonia simplicifolia agglutinin-I and Phytolacca americana mitogen may represent useful tools in distinguishing poorly differentiated squamous cell carcinomas from other poorly differentiated mammary epithelial tumors.  相似文献   
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Twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. Histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with Arachis hypogea agglutinin (peanut agglutinin; PNA), Concanavalia ensiformis agglutinin (conA), Dolichos biflorus agglutinin (DBA), Glycine max agglutinin (SBA), Griffonia simplicifolia agglutinin-I (GS-I), Lens culinaris agglutinin (LCA), Lycopersicon esculentum agglutinin (LEA), Phytolacca americana mitogen (pokeweed mitogen; PWM), Ricinus communis agglutinin-I and -II (RCA-I and -II), Triticum vulgaris (WGA), and Ulex europaeus agglutinin-I (UEA-I). Each lectin had a specific binding pattern, except SBA and DBA. In nonlactating glands, PNA, conA, LEA, and UEA-I stained duct cells in a linear-binding pattern, with a mean percentage of positive ducts per section of 28.7 (+/- 0.6), 65.7 (+/- 0.3), 100 (+/- 0), and 8.4 (+/- 0.2), respectively. Strong apical, lateral, basal, and cytoplasmic positivity on duct cells was seen after incubation of the sections with RCA-I, RCA-II, and WGA in all ducts. In acinar cells, the binding pattern and the staining distribution of all the lectins studied were similar to those in duct cells. However, for PNA, conA, and UEA-I, the mean percentage of positive lobules per section was 33.7 (+/- 0.9), 62 (+/- 0.5), and 10.5 (+/- 0.2), respectively. In glands from lactating dogs, conA and UEA-I did not stain. The cytoplasm of all myoepithelial cells was moderately stained with RCA-I, RCA-II, and WGA.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   
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  1. Recent ROV surveys conducted on the Ragusa bank in the Sicily Channel (Mediterranean Sea) documented illegal and continuous use of the ‘ingegno’ gear for red coral fishing.
  2. Three lost gears were recorded showing different levels of epibiosis thus supporting multi‐annual exploitation of the bank.
  3. The red coral population of the Ragusa bank showed a typical ‘forest‐like’ configuration with upright, medium‐sized colonies similar to those of other commercially exploited deep Mediterranean banks.
  4. The direct negative effect of this trawling gear was evident from numerous rocky boulders completely enveloped by lost nets and occasionally turned over. Red coral fragments as well as fragments of ‘ingegno’ nets were observed in high abundance on the sea floor. This evidence suggests that this gear is highly destructive even on rough sea floors, contrary to the traditional view, which is that it has a more random impact on rough rocky bottoms.
  5. The estimated coral uptake of ‘ingegno’ is 45% of the total coral biomass, while the estimated coral loss, in terms of fragments, is 9%, demonstrating the high impact of this gear.
  6. The study highlighted the infringement of the red coral fishing ban by trawling gears as well as a lack of effective policing. The strengthening of controls on landings at nearby harbours, and education programmes for the local maritime communities are among the management actions proposed to protect these surviving red coral populations from the ongoing illegal fishing activity.
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