体重决定猪回肠内的蛋白吸收率

<正>近期伊利诺伊大学的J.A.Almeida博士、 Hans H.Stein博士以及Carsten Pedersen博士联手展开体重对于标准化回肠蛋白质和氨基酸吸收率的影响试验,结论证明,20 kg以下的仔猪回肠对于粗蛋白和氨基酸的吸收率比20~50 kg的育成猪和50~110 kg的育肥猪低。而且同一猪群的不同猪只也表现出吸收率的差异性。例如,在测定的300只仔猪中粗蛋白和氨基酸的吸收率     

Investigation of the allergenic potential of wines fined with various proteinogenic fining agents by ELISA

Hidden allergens are a common problem in food safety that has been known for many years. This is why the European Parliament adopted Directive 2003/89/EC amending 2000/13/EC. In addition to specific ingredients, Directive 2003/89/EC also requests the declaration of specific products that were used in the production and could be a risk for allergic individuals. This also includes the declaration of fining agents and lysozyme used in wines. In fact, it could be assumed that fining agents would be almost completely removed during the manufacturing process; however, until now there has been no necessity to analyze wine for these fining agents. By applying enzyme-linked immunosorbent assay (ELISA), residuals of fining agent proteins and the stabilizer lysozyme were investigated in various German wines. The results showed no detectable amounts of fining agents in wines, except for dried egg white and lysozyme, both derived from hen's egg white. For those products, adverse reactions against treated wines could not be excluded.     

Nitrogen turnover and N2O production in the forest floor of beech stands as influenced by forest management

Effects of forest management (thinning) on gross ammonification, net ammonification, net nitrification, microbial biomass, and N₂O production were studied in the forest floor of adjacent untreated control (“C”) and thinned (“T”) plots in three beech (Fagus sylvatica L.) stands in the Swabian Jura (Southern Germany) during three intensive field campaigns in the year 2004. The investigated sites are located less than 1 km apart on the slopes of a narrow valley. Due to different exposure (southwest, northeast, northwest), the three sites are characterized by warm‐dry microclimate (southwest site, SW) and cool‐moist microclimate (northeast site, NE; and northwest site, NW). Measurements at the NW site covered the second year (13 to 20 months) after thinning, and measurements at the SW and NE sites covered the sixth year (61 to 68 months) after thinning. Mean gross ammonification varied insignificantly across the six plots (range: 37.5 ? 31.2 to 51.0 ? 10.5 mg N (kg dry soil)^–1 d^–1). The SW site was characterized by very low net nitrification and nitrate (NO ) concentrations that were not significantly different between control and thinned plot. In contrast, for the thinned plot at the NE site (NET), significantly increased mean net nitrification (2.3 ? 1.2 mg N (kg dry soil)^–1 d^–1 at the NET plot vs. 0.4 ? 0.2 mg N (kg dry soil)^–1 d^–1 at the NEC plot) and mean extractable NO concentrations (43.9 ? 22.8 mg N (kg dry soil)^–1 at the NET plot vs. 4.1 ? 0.8 mg N (kg dry soil)^–1 at the NEC plot) were observed. The differences in net nitrification and NO concentrations across the research plots were related to differences in the forest‐floor C : N ratios: net nitrification increased exponentially below a threshold C : N value of about 25. The results of this study indicate that the forest floor of the warm‐dry SW site is very resistant to N loss triggered by thinning due to high C : N ratios around 30. Under the cool‐moist microclimate of the NE site, a significantly lower C : N ratio of 22.1 at the thinned plot (control plot: 26.7) coincided with significantly increased net nitrification. Thus, different responses of net nitrification to thinning under different microclimate appear to be triggered by different C : N ratios. Nitrous oxide production was mainly governed by forest‐floor water content, and since differences in water content at adjacent control and thinned plots were low, N₂O production was not significantly different between adjacent control and thinned plots.     

Crown allometry and growing space requirements of four rare domestic tree species compared to oak and beech: implications for adaptive forest management

European Journal of Forest Research - Rare domestic tree species are increasingly being viewed as promising alternatives and additions to current main tree species in forests facing climate change....     

Correction to: Silvicultural prescriptions for mixed-species forest stands. A European review and perspective

European Journal of Forest Research -     

Short-term storage and cryopreservation of milt from Atlantic salmon and sea trout

Experiments on short-term preservation of sperm were performed with Atlantic salmon (Salmo salar). Fertility was maintained for up to 10 days when 2 mm thick samples were stored at 0° C under an oxygen atmosphere in the presence of antibiotics (125 IU penicillin and 125 μg streptomycin per ml sperm). Fertility was completely lost after 24 days. Sperm stored without antibiotics fertilized 100% of eggs after 6 days.

Cryopreservation was carried out with milt from Atlantic salmon and sea trout (Salmo trutta). Semen mixed with extender was frozen on dry ice (pellets) with subsequent storage in liquid nitrogen. Sperm pellets were thawed in a 0.12-M NaHCO₃-solution (10° C) before insemination. The suitability of an extender as previously described by Stoss and Holtz and of a 0.3-M glucose solution with the addition of 10% DMSO, was tested on two different batches of sperm and eggs in Atlantic salmon and sea trout. In addition, the extender earlier reported by Mounib and an aqueous solution of 10% DMSO were only used in Atlantic salmon with one batch of gametes.

Insemination with cryopreserved Atlantic salmon sperm resulted in 36 to 91.3% eyed eggs (control = 100). The differences were caused by the type of extender and the batch of gametes employed. The very simple extender consisting of 0.3 M glucose and 10% DMSO only was the most successful. Results with cryopreserved sea trout sperm ranged between 38.6–54.8% eyed eggs, showing no difference between treatments.     

Broken eggs as a cause of infertility of coho salmon gametes

Egg content originating from ruptured ova progressively reduced the success of fertilization in coho salmon (Oncorhynchus kisutch) eggs. The salmon egg differs in ionic composition from the ovarian fluid in exhibiting higher K⁺ and Mg²⁺ and lower Na⁺ concentrations. Normal ovarian fluid induced spermatozoan motility whereas ovarian fluid contaminated with egg content progressively lost the ability to induce motility. Loss of motility was related to decreasing $\text{Na}{}^{\mathrm{+}}\text{K}{}^{\mathrm{+}}$ ratios in the ovarian fluid, indicating that reduced fertility may be caused by inactivation of sperm cells in the insemination medium. Removal of contaminated ovarian fluid by rinsing ova in isotonic media induced spermatozoan motility and restored fertility completely. The use of an isotonic NaHCO₃ solution for rinsing of ova is suggested as a routine procedure for artificial insemination in salmonids where the presence of broken eggs is suspected.