汉江多参数水质连续自动在线监测系统

介绍了汉江多参数水质在线监测系统的研制开发，分析了系统的基本原理和整体设计方案，介绍了系统硬件设计方案以及软件设计。水质参数数据经过存储和网络传送以及基于人工智能的预测分析，相互传输数据，实现了对汉江水质的监控和预测，为建立完备的汉江水质监测数据库打下基础，同时为有关部门对汉江水环境的治理提供依据。     

新型挂面包装机的输送和称重设计

在许多领域的应用中，自动称重扮演一个重要组成部分，在交易中心大量的商品和原料的处理，散装到散装称重过程使用自动皮带秤或总计料斗秤，数量较小的商品则通过重力灌装机械或分检秤自动称重和灌装到终端用户．由车辆运送或经铁路运输的商品往往分别用运动道路称重装置或自动轨地秤称重。一种新型挂面包装机采用带式输送装置、称重传感器，使挂面包装机结构简洁，输送稳定，称重精确，易于实现自动化控制，具有良好的性价比。     

早熟金针菇新品种G1的杂交选育

以金针菇菌株F3-31和FM-83为亲本,经过杂交选育出优良白色金针菇菌株G1.以菌丝生长速度、生长势、产量、子实体农艺性状及栽培周期等为指标,对金针菇菌株G1与其亲本进行比较.试验结果表明,G1菌丝生长速度快且生长势旺盛;平均单瓶产量达216 g,比亲本分别增产54 g、20 g,生物转化率也比亲本菌株高,达到72%;栽培周期43 d,比亲本分别缩短1 d、6 d;子实体各项农艺性状优良,是适于金针菇工厂化栽培的早熟白色优良菌株.     

西瓜EST-SSR分子标记的筛选

利用国际共享获得的820条EST序列,自主设计出79对EST-SSR引物,其中有32对引物经过PCR扩增出稳定清晰的电泳条带,可以作为西瓜和相关物种的新的EST-SSR分子标记。     

利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST

 以辣椒(Capsicum annuum L. ) 细胞质雄性不育系23A、121A, 和其相应的近等基因恢复系23C、121C为试验材料, 利用抑制消减杂交( SSH) 技术成功构建了CMS恢复基因诱导表达的消减cDNA文库。结合高密度点阵膜杂交差异筛选, 获得了282个阳性克隆。通过测序, 除去重复序列共得到175个Unique ESTs。在GenBank上进行BLAST分析, 55个EST片段未找到对应的同源序列, 可能代表了新基因;120个EST片段找到了对应的同源序列, 包括103个已知功能基因和17个未知功能基因。按照MIPS功能分类法, 将其分为14个功能组, 涉及代谢、胁迫应答、蛋白活性、转录因子、信号转导等多方面的功能。     

Relationship between zinc and zinc transporter expression in human lung cancer cells

AIM: To study the changes of zinc transporter gene expression in A-549 cell line exposed to ZnCl2 and N,N,N’,N’-tetrakis 2-pyridylmethyl ethylenediamine (TPEN).METHODS: Human lung cancer cell line A-549 was exposed to different concentrations of ZnCl2 (0, 50, 100, 150, 200 μmol/L) and TPEN (0, 5, 10, 15, 20 μmol/L), respectively. Twelve hours later, the cell viability was measured by MTT (methyl thiazolyl tetrazolium) and levels of zinc transporter mRNA was detected by RT-PCR. Zinquin was used to estimate the intracellular zinc concentrations.RESULTS: A-549 cell viability rate was significantly decreased when exposed to ZnCl2 at concentrations of 150 and 200 μmol/L, and to TPEN. The intracellular zinc concentration was significantly increased when exposed to ZnCl2 and decreased when exposed to TPEN. Zinc transporter (ZnT-1) mRNA level was increased along with the increase in the concentration of ZnCl2 but decreased when exposed to TPEN. The expressions of ZIP-1 and ZIP-10 (Zrt-and Irt-like protein) were increased along with the increase in the concentration of TPEN but decreased when exposed to ZnCl2.CONCLUSION: ZnT-1 expression is induced by zinc supplement. ZIP-1 and ZIP-10 expressions are induced by zinc deficiency and repressed by zinc supplement.     

Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

一例奶牛皱胃溃疡伴低血钾综合征的临床治疗

2005年12月郑州牧业工程高等专科学校附属兽医院接诊了1例皱胃溃疡伴低血钾综合征的奶牛，该牛同时患有低血钙、酮病等。病情发展中患牛在血钾含量为2.28 mmol/L时四肢无力，卧地不起，通过补钾及综合治疗血钾升至3.45 mmol/L时又能自主站立，对皱胃溃疡采取消炎、抑制胃酸分泌、收敛止血等方法进行治疗。