Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.     

亏缺灌溉对南疆棉花生长和水分利用的影响

针对南疆地区水资源短缺、作物水分利用效率低等问题,以棉花为试验材料进行田间小区试验,在棉花现蕾期、开花期以及结铃期分别设置3个亏缺灌溉水平(W₁:50%ET_c,W₂:65%ET_c,W₃:80%ET_c,ET_c为作物蒸发蒸腾量),以全生育期100%ET_c灌溉处理为对照(CK),研究膜下滴灌条件下,不同生育期亏缺灌溉对棉花生长、产量、氮素吸收和水分利用效率的影响.结果表明:现蕾期亏水对棉花株高、叶面积指数、地上干物质生长、氮素吸收和产量有不同程度的抑制效应,但复水后补偿效应显著,其中轻度亏水(W₃)在籽棉产量减少3.48%的条件下,WUE高达1.57 kg/m³,显著高于CK的1.48 kg/m³;开花期亏水,棉花的各项生长指标均有显著降低,复水后补偿效应不显著,不利于棉花生长发育;结铃期亏水对棉花地上干物质累积、氮素吸收和产量均有显著的抑制效应,但在W₂和W₃水平下,WUE均达1.51 kg/m³.综合考虑在保证棉花产量的同时达到节水增产的目的,可在棉花蕾期进行80%ET_c灌水,其他生育阶段实施充分灌溉,来控制营养生长,促进生殖生长,获得更高的水分利用效率.     

Cepharanthine induces autophagy via PI3K/AKT/mTOR signaling pathway in ovarian cancer SKOV3 cells

AIM: To investigate the autophagy of human ovarian cancer SKOV3 cells induced by cepharanthine and to explore its mechanism. METHODS: The effect of cepharanthine on the viability of ovarian cancer SKOV3 cells was measured by CCK-8 assay. The SKOV3 cells were treated with cepharanthine, and then the formation of autophagosome was observed with acridine orange staining under fluorescence microscope. The protein levels of LC3, AKT, p-AKT, mTOR, p-mTOR and GAPDH in the SKOV3 cells treated with cepharanthine were determined by Western blot.RESULTS: Cepharanthine significantly inhibited the viability of ovarian cancer SKOV3 cells in a dose-dependent manner (P<0.05). The number of the intracellular acidic autophagosomes with bright red fluorescence was significantly increased after cepharanthine treatment in the SKOV3 cells. The expression of LC3-Ⅱ in SKOV3 cells was significantly enhanced after cepharanthine treatment. Furthermore, treatment with cepharanthine in the SKOV3 cells also resulted in a significant down-regulation of phosphorylated form of AKT and mTOR (P<0.01), while the total protein level was not changed. Combination of cepharanthine and 3-methyladenine resulted in a substantial decrease in the cell viability compared with using cepharanthine alone.CONCLUSION: Cepharanthine significantly inhibits the growth of human ovarian cancer SKOV3 cells and induces the autophagy, which may be correlated with down-regulation of PI3K/AKT/mTOR signaling pathway.     

关于动物医学专业少数民族学生教育的探析

分析了动物医学专业少数民族学生教育教学面临的困难及存在的问题，提出了调整少数民族班级的培养方案、民汉帮学提高少数民族学生汉语水平、提高少数民族学生的英语应用能力、提高动物医学专业少数民族学生就业质量等具体改革措施，从而达到提高少数民族学生对动物医学专业的理解、提升学生的主动学习意识、提高学生的综合能力、促进学生就业的目的。     

棉花“保险+期货”模式助力南疆主产棉区脱贫攻坚

通过解析棉花“保险+期货”模式的运行机制、优势及存在的不足，并提出了发展优质棉“订单生产+保险+期货”模式和引入银行、基金等信贷机构的建议，期望对南疆主产棉区贫困棉农增收致富、助力脱贫攻坚提供有益探索。     

应用SYBR Green I的Real-time PCR方法定量检测空肠弯曲杆菌

空肠弯曲杆菌(Campylobacterjejuni)被认为是人类主要食源性病原菌之一。由于空肠弯曲杆菌特殊的生长条件和容易进入不可培养但存活的状态(Viablebutnonculturable,VNC),所以传统的生化鉴定结果不一定可靠,并且是一项费时而繁琐的工作。核酸检测方法的出现为空肠弯曲杆菌的检测带来了方便。本文基于LightCycler为平台,利用SYBRGreenI染料能特异与双链DNA结合而发荧光的特性,建立一种Real-timePCR方法来定量检测空肠弯曲杆菌。用该方法检测时,发现所有空肠弯曲杆菌(11株)都呈阳性,所有其它弯曲菌(3株)和其它菌株(5株)都是阴性。整个检测过程60min内可以完成,检测限度为5CFU,标准曲线的相关系数为1。结果表明基于SYBRGreenI的定量PCR方法既为空肠弯曲杆菌提供了一种特异、敏感、快速和简洁的定量检测方法,又为研究空肠弯曲杆菌致病机理提供了一种重要方法。     

肉鸡腹水综合征的心电图学研究进展

近年对肉鸡腹水综合征的研究已取得进一步的认识 ,在研究肺动脉高压的同时更注意具有潜在作用的心脏病变的研究。家禽的心脏传导系统比较特殊 ,它含有一个返回分支束 ,使右房室瓣受传导神经的支配。当右房室瓣不受传导神经的支配时 ,瓣膜功能不全而引起右心室容量过大 ,可介导右心室肥大。心电图描记是研究肉鸡腹水综合征发病机理的有效手段之一。文章系统地阐述了肉鸡心脏的形态结构和传导系统的特点以及几种肉鸡腹水综合征试验模型的心电图学特征、肉鸡的心脏病理变化与心电图学特征之间的关系     

湘西北美味牛肝菌的生态研究

美味牛肝菌菌体较大 ,菌肉肥厚 ,菌柄粗壮 ,食味浓香可口 ,属珍贵食用菌之一。不仅可食用 ,也可药用。本文就笔者 2 0 0 0年 5月以来 ,对湘西北美味牛肝菌的分布及生态研究的结果予以报告。     

猫鼻气管炎病毒、怀状病毒、泛白细胞减少症病毒的分离及其其形态学观察

根据病毒的理化特性，从猫三联活疫苗中分离出猫鼻气管炎、猫西洋太和猫泛白细胞减少症病毒；筛选出每种病毒敏感的细胞，并将该3种病毒分别分别在其中传代与扩增；通过电镜观察此3种病毒的形态学特征及其在宿主细胞内增殖部位、分布情况等，并以此对猫3种病毒进行了初步鉴定。     

Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.