C语言教学现状剖析与改革

C语言是大学计算机专业的一门重要的必修课,通过对C语言的学习让学生们能初步掌握程序设计的原理与方法。本文通过阐述目前大学C语言的教学发展情况,剖析了存在的问题并提出了改革的建议。     

CD147 monoclonal antibody-mediated nanoparticles for gene therapy to target lung cancer cells

AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells.     

兴宁市粮食增产模式攻关试点工作方案

介绍了兴宁市粮食增产模式攻关试点总体思路、基本原则和目标任务,结合该市市情,进一步阐述了兴宁市粮食增产模式攻关的技术模式和攻关内容,为兴宁市粮食增产模式攻关和高产创建提供参考。     

滑动平均法和分形理论结合的稻瘟病发病特性研究

通过文献分析稻瘟病发生与气象有着密切的关系,利用滑动平均法将云南省凤庆县稻瘟病病情指数分解成趋势及气象波动病情指数,分析结果表明趋势病情指数总体呈下降趋势,而气象波动病情指数具有一定的混沌特性,并利用相空间延拓及G-P算法验证了气象波动具有一定混沌特性。     

玉米-大豆带状套作对大豆叶片形态及光合特性的影响

研究玉米-大豆带状套作下大豆不同生育时期的叶片形态和光合特性,探讨叶片对带状套作的响应机制,为耐荫大豆品种筛选与选育提供依据。以南豆12、桂夏1号和华夏5号等3个耐荫性存在显著差异的大豆品种为试验材料,在大田试验条件下,活体测定植株叶倾角、叶柄长度、叶形指数和光合特性,取样测定大豆叶片的比叶重和单株叶面积。结果表明:与单作相比,玉米大豆带状套作条件下,大豆叶倾角显著增加,苗期和花期上层增幅大,荚期则为下层增幅最大;叶形指数在苗期和花期显著增加,不同生育时期各品种不同层位对套作环境的响应程度不同;叶柄长度从大到小均依次表现为上层、中层、下层,且在套作下显著增加,其中苗期和花期上层增幅最大;比叶重和单株叶面积减小,大豆胞间CO2浓度上升,叶片净光合速率、气孔导度和蒸腾速率均显著下降。不同品种对带状套作的响应程度不同,强耐荫品种南豆12叶倾角增幅大而叶柄长度增幅最小,比叶重、单株叶面积和净光合速率下降幅度均为最小;荫蔽敏感型品种华夏5号叶柄长度增幅最大而叶倾角增幅最小,叶片胞间CO2浓度显著增加,净光合速率和单株叶面积下降均最为明显。强耐荫品种南豆12在玉米大豆带状套作模式下,能够通过调节自身叶片形态适应环境变化,光合速率下降幅度较小,从光能截获和利用方面均表现出了对套作较强的适应能力。     

四川大豆疫霉毒力鉴定及资源抗性分析

采用土壤诱捕和组织分离的方法,从四川省仁寿县和雅安市大豆根腐病发病田块采集的土壤中和病株上分离到4个大豆疫霉分离物RS1、RS2和YA1、YA2,用含有已知抗性基因的鉴别寄主进行毒力鉴定。结果表明YA1和YA2毒力公式相同,为1a,1b,1d,2,3c,属于同一致病型;RS1、RS2毒力公式分别为1a,1d,1k,3a,3c,4,5和1a,1b,1c,1d,1k,2,3c,5,分别为不同致病型。用3个致病型接种157份大豆资源,结果表明,抗病种质相对较少,占23.57%;地方品种抗病性较育成品种强,本研究可为大豆抗病性育种提供科学依据。     

湖北省茄子品种抗褐纹病鉴定

收集了目前湖北省市场上销售的34个茄子品种,采用室内离体茄果人工接种的方法,鉴定了这些品种对茄子褐纹病的抗性水平。结果显示:从34个茄子材料中鉴定出免疫品种8个,高抗品种4个,抗病品种2个,中抗品种2个,感病品种18个,说明湖北市场上推广种植的茄子品种50%以上为感褐纹病品种。     

辣椒新品种航椒18号的选育

航椒18号是以051-3-2-2-H-H-H为母本,以035-1-2-2-H-H为父本杂交育成的早中熟羊角型辣椒一代杂种。从定植至青熟果采收55d(天)左右,始花节位为第9~11节。果面皱、果肩宽、有光泽,味香辣;单株结果数20个左右,单果质量50.5g左右;果实粗长羊角形,纵径22.7cm,横径4.2cm,肉厚0.25cm。田间对辣椒病毒病、炭疽病和疫病的抗性强于对照陇椒3号。平均每667m~2产量4200kg,适宜在甘肃、内蒙古、新疆等地及生态环境相似区域保护地栽培。     

氮肥对辣椒品质的影响

为探讨氮肥与盆栽辣椒品质的关系,以辣椒品种"津福35"和"豫艺7"为试材,研究了不同施氮水平(每盆尿素施用量分别为0.76、1.52、2.28g)对温室盆栽辣椒果实中硝态氮、可溶性糖、可溶性固形物、可溶性蛋白质含量的影响。结果表明:不同施氮水平对辣椒的品质有不同的影响,硝态氮、可溶性固形物、可溶性蛋白质含量均随着氮肥的施入量增加先降低后升高;可溶性糖含量呈增长趋势,以期为辣椒设施栽培提供一定的理论依据。     

三种五加在青海不同地区的引种栽培表现

以刺五加苗、短梗五加苗、红毛五加为试材,分别在大通县、互助县、西宁市进行引种栽培试验,通过3年对3种五加的成活率、物候、冠幅、树高、地径等生长指标观测,研究不同试验地对3种五加的引种栽培表现。结果表明:引种的3种五加在试验地均能完成正常的开花结果等生育期,引种驯化初步成功;不同试验区,物候期及各项生长指标发生较为明显变化。3种五加在同一地区物候期基本表现相似,但略有不同。3种五加可在青海西宁地区及大通河流域的主要林区根据实际需要选择栽培。