Evaluation of a Burdizzo Castrator for Neutering of Dogs

A Burdizzo castrator was evaluated for the neutering of dogs. Histological and morphological changes of spermatic cells and peripheral serum testosterone after challenge with a GnRH-analogue (gonadorelin) were assessed. There was a control group (G1), a surgically castrated group (G2) and a Burdizzo group (G3) divided in two, G3a receiving two crunches in each spermatic cord and G3b receiving one crunch in each spermatic cord. Sixteen days after application of the Burdizzo blood samples were taken from the dogs at 30 min interval during 2 h; after the second sample the dogs were treated with 1 mug/kg body weight of gonadorelin i.v. The same protocol of gonadorelin challenge was performed in G1 and G2 dogs. The G2 dogs were surgically castrated after the second blood sample, before the gonadorelin treatment, and the G1 dogs after the last blood sample. The excised gonads were examined histologically, and sperm smears were prepared from the caudae epididymidis. The testes and plexus pampiniformis of the G1 and G2 dogs had a normal histological appearance, and they had morphologically normal epididymal sperm cells. In all G3 dogs, there was an acute fibrosis with an inflammatory reaction in the plexus pampiniformis. The testes from the G3a dogs showed diffuse areas of infarction and degeneration of the parenchyma. Similar but less diffuse lesions were seen in group 3b dogs. The deferent ducts from all G3 dogs showed vasitis and/or sperm granulomas. Azoospermia or sperm malformations were observed in the epididymal smears from the G3 dogs. Testosterone concentration in the G1 dogs increased after gonadorelin application (p < 0.0001). The G2 dogs had basal testosterone levels after castration (p < 0.001) and did not respond to gonadorelin. Groups 3a and b showed a slight but non-significant increase in testosterone concentration after gonadorelin challenge, supposedly due to the reduction of testicular blood flow and loss of testicular interstitial tissue.     

农业用地供给侧改革的可行举措探讨

通过梳理我国农业用地在利用中所存在的制度层面、利用层面、管理层面和其他层面的问题,结合我国推进农业用地实行供给侧改革的目的,从4个方面制度供给、利用供给、管理供给和其他供给提出我国在实行农业用地供给侧改革的可行举措,为我国推进农业用地的供给侧改革提供理论依据。     

Biodiversity assessment and conservation strategies

The efficient representation of all species in conservation planning is problematic. Often, species distribution is assessed by dividing the land into a grid; complementary sets of grids, in which each taxon is represented at least once, are then sought. To determine if this approach provides useful surrogate information, species and higher taxon data for South African plants and animals were analyzed. Complementary species sets did not coincide and overlapped little with higher taxon sets. Survey extent and taxonomic knowledge did not affect this overlap. Thus, the assumptions of surrogacy, on which so much conservation planning is based, are not supported.     

《现代养殖设施与设备》教材建设探索与实践

针对《现代养殖设施与设备》课程没有规范教材的现状，探索符合人才培养目标和规格、适应市场需求的特色应用型教材建设，为应用型人才培养提供知识载体。并通过进一步实践，为教材的修订完善明确了方向。     

Long‐term effects of organic manure and manufactured fertilizer additions on soil quality and sustainable productivity of finger millet under a finger millet–groundnut cropping system in southern India

In a 20‐yr‐old long‐term experiment, the impact of continuous application of organic manures and inorganic fertilizers on soil quality and the sustainability of finger millet production was conducted on two cropping systems: finger millet and finger millet–groundnut on an Alfisol of semi‐arid southern India. The study was conducted from 1992 to 2011 at the All India Coordinated Research Project for Dryland Agriculture, UAS, Bangalore, using a randomized block design. The treatments comprised of T₁: control [no fertilizer and no farmyard manure (FYM) applied], T₂: FYM 10 t/ha, T₃: FYM 10 t/ha + 50% of recommended NPK (50:50:25 kg/ha), T₄: FYM 10 t/ha + 100% of recommended NPK and T₅: 100% recommended NPK. Comparison of long‐term yield data between treatments was used to calculate a ‘sustainability yield index’ (SYI), which was greatest for T₄ (FYM 10 t/ha + 100% of recommended NPK), in both rotational (0.68) and monocropping (0.63) situations. Soil quality indices were determined using principal component analysis linear scoring functions. The key indicators which contributed to the soil quality index (SQI) under rotation were organic C; potentially available N; extractable P, K and S; exchangeable Ca and Mg; dehydrogenase activity and microbial biomass C and N. The largest SQI (7.29) was observed in T₄ (FYM 10 t/ha + 100% NPK), and the smallest (3.70) SQI was for the control. Application of 10 t/ha FYM together with NPK (50:50:25 kg/ha) sustained a mean yield of 3884 kg/ha.     

Molecular characterization of an influenza A virus (H4N2) isolated from waterfowl habitats in the State of Mexico

Wild waterfowl and their habitats are the main reservoirs of influenza A virus (IAV) mainly during the breeding season and prior to migration. This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico. The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America. This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.     

Loss of Restriction Site DdeI, used for Avian Molecular Sexing, in Oreophasis derbianus

Molecular sexing is a rapid and safe procedure for bird sex determination. Two universal methods based on the amplification of a chromo‐helicase‐DNA‐Binding 1 (CHD) gene region, located in both sexual chromosomes (Z and W), have been established. We found that molecular sexing of Oreophasis derbianus failed by using these two procedures. One of them is based on a restriction site located in CHD1W gene but absent in CHD1Z. The DdeI restriction site, used successfully to determine gender in several bird species, was found to be lost because of nucleotide change in O. derbianus. This change created a new restriction site, NlaIII, that was successfully applied for sexing this endangered bird.     

Fibrin–alginate hydrogel supports steroidogenesis,in vitro maturation of oocytes and parthenotes production from caprine preantral follicles cultured in group

This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In a first experiment, follicles were encapsulated as a single unit per bead and cultured singly or in groups or with five follicles in the same alginate (ALG) bead for 18 days. In a subsequent experiment, the “five follicles per bead” design was chosen to culture in ALG, fibrin–alginate (FA) or hyaluronate (HA) for 18 days. In a third experiment, we chose the five follicles per bead in FA to culture for 30 days. The culture set‐up of five follicles per ALG bead increased antrum formation and follicle diameter compared to the other culture designs (p < .05). Moreover, under this condition, 44.44% of the oocytes from in vitro cultured preantral follicles reached meiotic resumption. A significant increase of follicle diameter occurred in attachment system and FA (p < .05), but the ALG condition reached the highest among all groups on day 18 (p < .05). Follicles encapsulated in matrix produced more estradiol and progesterone than attachment system (p < .05). The expression of MMP‐9 mRNA was higher in FA than in other groups (p < .05) and similar to antral follicles from in vivo control (p > .05). Only FA group resulted in oocytes matured. After 30 days, oocytes from preantral follicles in vitro grown in FA developed to eight‐cell parthenotes. In conclusion, a culture system using FA supported the development of caprine preantral follicles cultured in group and included in the same bead of hydrogel, improving the oocyte maturation and producing parthenotes.     

Solarization for the control of Pueraria montana (kudzu)

The effects of translucent polyethylene sheeting as a thermal covering to eradicate Pueraria montana (kudzu) were investigated at Clemson, South Carolina on a clay loam. In 2005, the highest reduction of live root crowns was observed where P. montana was covered for the entire growing season (May–October) with a reduction of 42% of live root crowns compared with the control plots. Where P. montana was covered for alternate weeks throughout the growing season live root crowns were reduced by 35%. Covering P. montana for one week and then uncovering for four weeks repeatedly through the season killed 24% of root crowns. In 2006, the second year of treatment to the same plots, the three solarization treatments had similar efficacy of about 97%. The use of polyethylene sheeting appears not to be cost‐effective for general control of large P. montana infestations, but may be useful for small patches.     

The effect of feeding time (day versus night) and feeding frequency on pancreatic exocrine secretion in pigs

The effect of night feeding and feeding frequency on exocrine pancreatic secretion was studied in five chronically catherized growing pigs (16 to 31 kg). Feeding during the night (2200 to 2400 h) as compared to the day (1000 to 1200 h) tended to stimulate cholesterol ester lipase activity and tended to lower the colipase : lipase ratio in the pancreatic juice, but no effect on volume output, protein output and the activities of trypsin, chymotrypsin, amylase, lipase and colipase could be demonstrated. Feeding 12 small meals between 0800 and 2000 h as compared to one large meal (1000 to 1200 h) daily, altered the pattern of exocrine pancreatic secretion, tended to stimulate protein output by 44 %, chymotrypsin activity by 29 % and lipase activity by 46 %. These observations strengthened the theory that exocrine pancreatic secretion is partly regulated by feed intake per se and does not only depend on the amount of feed consumed. Feeding 12 small meals versus one large meal, compared at the same total daily feed intake, lowered the colipase : lipase ratio by 32 %. It can be concluded that feed intake pattern affected exocrine pancreatic secretion.