Expression of IGFBP2 and IGFBP4 in hepatocytes with experimental injury

AIM:To explore the mechanism of IGFBP2 and IGFBP4 in hepatocytes with injury induced by TNF-α and TGF-β1. METHODS:Human hepatocyte line (HL-7702) was cultured in vitro and treated with TNF-α or TGF-β1 for 24 h. The expression of IGFBP2 and IGFBP4 was detected by immunochemistry staining. The inhibition ratio of hepatocytes was detected by MTT assay. HL-7702 was treated with TNF-α at concentration of 20 μg/L for 48 h, then the apoptosis of hepatocytes was detected by both Annexin-V /PI and TUNEL assay. RESULTS:The expression of IGFBP2 and IGFBP4 in TNF-α or TGF-β1 treated groups was significantly higher than that in control group (P<0.05). The positive staining of IGFBP2 and IGFBP4 in TNF-α (20 μg/L) treated groups or TGF-β1 (4 μg/L) treated groups was the strongest among all groups. A positive correlation was found between IGFBP2 or IGFBP4 and inhibitory ratio of hepatocytes (P<0.05 or P<0.01). Compared with normal control group, the percentage of apoptosis markedly increased in TNF-α (20 μg/L) treated group (P<0.01). CONCLUSION:IGFBP2 and IGFBP4 involved in the injury process in hepatocytes, indicating an important role in injury of hepatocytes induced by TNF-α or TGF-β1.     

Effect of fractalkine on the expression and secretion of MMP-2 in human monocytes

AIM: To study the effect of fractalkine (CX3CL1, Fkn) on the expression and secretion of matrix metalloproteinase-2 (MMP-2) in cultured human monocyte line U937 cells. METHODS: The cultured U937 cells were incubated with recombinant human Fkn, the supernatant of human renal mesangial cells (HRMC) and Fkn neutralizing antibodies for 24 h. The mRNA expression of MMP-2 was analyzed by RT-PCR. The production of MMP-2 in the supernatant was analyzed by gelatin zymography. RESULTS: The level of MMP-2 mRNA and protein in the cells incubated with recombinant human Fkn decreased compared to control group. Similarly, the level of MMP-2 mRNA in the cells incubated with the supernatant of HRMC reduced compared to control group. However, the level of MMP-2 mRNA and protein in the cells incubated with the supernatant of HRMC adding Fkn neutralizing antibodies increased compared to that incubated with the supernatant of HRMC. CONCLUSION: Fkn inhibits the expression and secretion of MMP-2 in cultured U937 cells. HRMC might mediate the expression and secretion of MMP-2 in U937 cells through Fkn.     

黑龙江省农机购置补贴工作实施成效及建议

介绍近几年黑龙江省农机购置补贴工作的实施措施和成效，指出购机补贴工作中存在的问题．并对今后的农机购置补贴工作提出几点建议，以促进全省农机结构更加趋向合理化，推动农业机械化水平进一步提高，实现粮食增产、农业增效、农民增收。     

农村变电站选址规划方法

农村变电站选址是电网规划的重要组成部分,在农网规划是起承上启下的作用,文中介绍最优化选址问题,并将优化选址的数学模型进行分类,又全面地比较分析应用于变电站选址方法的诸多算法,提出5项有利于电力市场环境下的农网变电站选址的建议.     

杉木新造幼林施肥方案优选的初步研究

杉木新造幼林不同施肥方案的试验结果表明,施肥对杉木新造幼树生长具有明显的促进作用。经多目标决策分析确定,造林前施基肥钙镁磷600 kg.hm-2、造林当年追肥施复合肥180 kg.hm-2为最佳施肥方案,其次为施基肥钙镁磷600 kg.hm-2、追肥施尿素180 kg.hm-2。生产上可根据实际情况选择应用。     

Collection,Evaluation,and Utilization of Cotton Germplasm in China

A total of 8193 accessions,including 6822 Gossypium hirsutum,350 G.hirsutum race (sub-spe-cies),385 of G.barbadense,378 of G.arboreum,17 of G.herbaceum and 41 wild species,of cotton germplasm are now maintained in China.This germplasm is kept in Beijing National Long-term Genebank and Anyang Cotton Medium-term Genebank.Live plants of the wild species are kept in Cotton garden at Hainan Island.     

安哥拉山羊级进杂交后代生活力初步观察

对安哥拉山羊及其级进杂交后代的生长发育、繁殖力、抗病性、采食习性等进行初步观察研究，结果表明：在试验场良好的饲养条件下，各代杂种羊生长发育正常，但繁殖力、抗病性、采食能力随级进杂交代数的增加有降低的趋势。据此，笔者认为，在陕北特定的生态经济条件下，培育毛用山羊新品种，级进杂交代数不宜太高，到Ｆ３代应停止级进，进行横交固定，而且在整个育种中，应始终注意饲养问题，以保证新品种培育成功。     

哲罗鱼精子的超微结构

应用透射电镜观察了乌苏里江哲罗鱼(Hucho taimen)精子的超微结构.哲罗鱼的精子由头部、中段和尾部组成.头部呈卵圆形,主要结构是细胞核.核前端无顶体,后端有植入窝,核中染色质致密,存在着不规则的网络状间隙.中段包括中心粒复合体和袖套.近端中心粒为9组三联微管结构,与远端中心粒相互垂直.袖套与细胞核后端相连,含有丰富的线粒体和囊泡,部分线粒体彼此融合,形成复合线粒体.尾部细长,主要结构是轴丝,为典型的"9 2"微管结构.尾部的近核段有许多囊泡包围着轴丝,远核段则无此结构.尾部有对称排列的波纹状侧鳍.     

坚持以人为本 优化教师管理

“以教师为本”是“以人为本”的体现，是学校发展的关键。实行“以人为本”的教师管理，要树立人才强校的办学理念，全面提高教师素质，改变绩效管理模式，为教师创造良好的学习、生活环境。