Cloning of Buffalo BMP1 Gene and its Expression Pattern Investigation in Different Tissues

Buffalo BMP1 gene was cloned in the present study, the BMP1 sequence was systemically analysed by bioinformatics techniques, and the expression level of BMP1 gene in different tissues were also assayed with Real-time fluorescence quantitative PCR (QRT-PCR).The results showed that with RT-PCR a 3 195 bp buffalo BMP1 gene was cloned and sequenced, including the whole ORF of 2 967 bp (coding 988 amino acid).The sequence multialigned results showed that buffalo BMP1 gene shared 99%, 96%, 96%, 96% and 95% of similar amino acid sequence with Bos taurus, Sus scrofa, Equus, Homa sapiens and Mus musclus, respectively.It was predicted that buffalo BMP1 protein contained a signal peptide domain, a preregion, a metalloproteinases domain, five complement-Uegf-BMP-1 domain (CUB domain) and two epitheloid growth factor-like domain (EGF-like domain).In addition, we also analyzed the expression level in different tissues through QRT-PCR, the results showed that BMP1 gene mRNA existed in all nine tissues with the most abundant expression in heart, followed by testis, ovary, genital ridge, while with lower amount of bone and other tissues, the minimal expression in liver was observed.     

2014年全国桑蚕新品种实验室鉴定（陕西点）报告

2014年春季对来自镇江蚕种场、苏州大学、无锡西漳蚕种场、山东广通蚕种集团有限公司及山东农业大学的5对桑蚕新品种,秋季对来自苏州大学、西南大学、中国农业科学院蚕业研究所的3对桑蚕新品种进行了实验室鉴定。通过龄期观察和生命率、茧质、产茧量调查与分析,客观反映参鉴品种在陕西试验点的饲育表现和性状特点。     

贵州省鸟类新纪录——栗树鸭

2020年4月14日,在贵州省贵阳阿哈湖国家湿地公园保育区内(106°39′0.13″E,26°30′28.46″N,1107 m)开展鸟类监测,于浅水沼泽区拍摄到2只雁鸭类,经鉴定为栗树鸭(Dendrocygna javanica)。通过查阅相关文献,该种在贵州省的分布尚未记录过,属贵州省鸟类新纪录。本文对发现地概况、形态特征、生境特征和分布现状以及对该物种的保护进行了简要讨论。     

氮添加对天山高寒草原土壤酶活性和酶化学计量特征的影响

为探究氮添加对高寒草原生态系统土壤酶活性的影响,于2018年在中国科学院巴音布鲁克草原生态系统研究站,选择4个氮添加水平(对照,N0,0 kg·hm^-2·a^-1;低氮,N1,10 kg·hm^-2·a^-1;中氮,N3,30 kg·hm^-2·a^-1;高氮,N9,90 kg·hm^-2·a^-1),开展土壤酶活性对氮添加响应的研究,分析土壤酶活性对氮添加的响应特点,土壤酶化学计量比以及土壤酶活性与土壤环境因子的关系。结果表明:与对照相比,氮添加在N3水平显著增加β-1,4葡萄糖苷酶(βG)、β-D-纤维二糖水解酶(CBH)和β-1,4木糖苷酶(βX)酶活性(P<0.05),N1和N3水平显著增加碱性磷酸酶(AKP)活性(P<0.05),N3水平显著降低多酚氧化酶(PPO)活性(P<0.05),氮添加对亮氨酸氨基肽酶(LAP)活性影响不显著,N3水平下显著增加N-乙酰-β-D氨基葡萄糖苷酶(NAG)活性(P<0.05)。相关分析表明,8种土壤酶活性均与土壤有机碳(SOC、NAG除外)和总磷(TP)显著相关,与土壤总氮(TN)不相关。研究区土壤酶活性C∶N∶P化学计量比为1∶1∶1.2,与全球生态系统的土壤酶活性C∶N∶P的比值1∶1∶1相偏离,表明该研究区土壤微生物生长受磷素限制。冗余分析(RDA)进一步揭示出土壤有机碳和土壤全磷含量是影响土壤酶活性的主要因子。     

种植密度对滴灌马铃薯生长、产量的影响

为了了解耕培土滴灌条件下种植密度对马铃薯生长、产量以及水分利用效率的影响.试验共设密度分别为7.28×10⁴ 株/hm²(RS25),6.67×10⁴ 株/hm²(RS35),5.55×10⁴ 株/hm²(CK)3个处理.结果表明:随着种植密度的增加,株高、茎粗、干物质积累量以及商品薯率均有降低的趋势;产量、水分利用效率随种植密度的增加表现出先增大后减少,其中RS35处理产量和水分利用效率均表现最高,分别达到47 325 kg/hm²和12.05 kg/m³;在马铃薯品质方面,种植密度对马铃薯粗蛋白含量的影响不具有统计学意义;淀粉和维生素C随着密度的大幅增加而降低,其中RS25处理的淀粉较CK降低了5.57%,RS25处理的维生素C较CK降低了7.96%,同时RS35与CK处理不具有统计学意义.综上所述,种植密度为6.67×10⁴ 株/hm²的RS35处理马铃薯高产优质,且水分利用效率最高,为黑龙江地区滴灌马铃薯较为适宜的种植密度.     

Hyperspectral Models for Estimating SPAD Value of Cotton Leaves under Waterlogging Stress

[Object] To setup the hyperspectral sensing models for estimating SPAD value of cotton leaves under waterlogging stress. [Method] Irrigation and drainage controllable plots were introduced to simulate the waterlogging stress treatment in the flowering and boll forming stage, during which the change characteristics of the cotton leaf spectral reflectance and SPAD value were observed after 1 d, 3 d, 6 d, 9 d waterlogging, respectively. To find out the hyperspectral sensing models for estimating SPAD value of cotton leaves under waterlogging stress, the correlation and regression relationships between SPAD value and spectrum parameters were analyzed. [Result] (1) The SPAD value of the fourth cotton leaf from the top was significantly lower than control when suffers from waterlogging for 3 d, when waterlogged 9 d the SPAD value decreased by around 15% compared with the control. (2) The cotton suffering from waterlogged damage in the flowering and boll forming stage caused the reflection peak in green light wave band became steep, while the near infrared spectral reflectance increased, and caused the reduction of red absorption and red edge position "blue shifts", the red edge position drifts towards short wave with 4～5 nm when suffers from waterlogging for 9 d. With increase of the waterlogged days, the red edge slope and red edge area increased with a maximum value at 6 d of waterlogging, meanwhile, the skewness and kurtosis of red edge increased. (3) After waterlogging, the SPAD value of the fourth cotton leaf from the top (chlorophyll content) had a remarkable correlation with red edge slope(Dr), red edge position(λr), green peak reflection(Rg), green peak position(λg), red well position(λo), blue edge area(SDb), yellow edge skewness(Sy), yellow edge kurtosis(Ky), red edge skewness(Sr), red edge kurtosis(Kr), etc. An experience linear, polynomial and exponential models for estimating SPAD value had been built through using the Sy, Sr, Kr as independent variables, respectively, their determination coefficient (R²) were greater than 0.9, and the root mean square error (RMSE) were less than 1; and an experience binary linear regression equation for estimating SPAD value had been built through multivariate regression using the λg, SDr/SDb(VI3), Sb, Sy, Ky as independent variables, the R² was as high as 0.973, and the RMSE was 0.393. [Conclusion] The model can be remote sensing model used as estimating leaf SPAD of cotton value under waterlogging stress.     

13个广西喜树种源的喜树碱含量分析

以13个广西喜树(Camptotheca acuminata)种源的嫩叶为试材,研究不同种源对喜树碱含量的影响。结果表明,不同种源的喜树碱含量有极显著差异,不同土壤类型和海拔种源间的喜树碱含量有显著或极显著影响。13个广西喜树种源的喜树碱含量从高到低排序为德保田林金秀隆林德峨兴安西林天峨永福凌云东兰隆林新州南丹灵川。德保、田林、金秀喜树种源是优良种源。     

CAD/CAM应用软件的选型

CAD/CAM应用越来越广泛,但对于CAD/CAM应用软件的选型是一些设计人员及企业面临的问题,文章介绍了CAD/CAM软件系统的组成,CAD/CVM应用软件的选型要素,企业选型时应注意的事项.     

屋面防水的施工要点与整改措施

防水工程一直是建筑施工中难以控制的分项,防水层关系到日后的质量问题,如渗水势必会对房屋的适用性、耐久性造成影响.防水层的试水试验相当重要,也做为检验防水层施工质量的依据,今后施工防水层一定要重视蓄水试验,按照设计及技术方案做好防水层的施工.     

美洲黑杨无性系苗期光合生理特性研究

研究了9个美洲黑杨无性系的光合生理特性,为优良新无性系的选育提供参考。采用LI-6400便携式光合作用测定系统测定美洲黑杨的光合作用日变化及其光响应曲线,利用非直角双曲线Farquhar模型、二次曲线及直线方式拟合光响应曲线,分析比较光响应特征参数及影响净光合速率因子。结果表明:各无性系的净光合速率均具有明显的日变化规律,tn01-78、tn01-38、tn04-n52呈双峰曲线现象,2个峰值出现的时间在11:00和14:00左右,其余呈单峰曲线,净光合速率最大值出现在12:00左右;不同无性系最大净光合速率、暗呼吸速率、表观量子效率、光饱和点和光补偿点的大小范围分别为20.50~31.42μmol·m-2s-1、1.67~3.19μmol·m-2s-1、0.055~0.279μmol·μmol-1、950.83~1 360.83μmol·m-2s-1和29.03~55.20μmol·m-2s-1;净光合速率(Pn)变化与气孔导度Gs、水压亏缺Vpdl、空气温度Tair和光合有效辐射VPAR呈正相关,与胞间CO2浓度Ci和大气相对湿度(HR)呈负相关;净光合速率与影响因子的相关程度依次为气孔导度>光合有效辐射>胞间CO2浓度>大气相对湿度>空气温度>水压亏缺。