大熊猫犬冠状病毒的分离与鉴定

以犬肾传代细胞(MDCK)从1只病死大熊猫肝脏中分离到1株病毒,命名为DXMV。电镜观察磷钨酸负染的病毒细胞培养物，发现冠状病毒样粒子，超薄切片上可见细胞浆内病毒包涵体。理化学和生物学鉴定结果表明，DXMV核酸类型为RNA，对氯仿、乙醚敏感，可耐受1％胰蛋白酶的作用，具有一定的耐酸性和耐热性，可在MDCK、FCWF、F81细胞中增殖，无血凝性和血吸附特性。血清中和试验结果表明，DXMV可被犬冠状病毒阳性血清中和。采用犬冠状病毒间接荧光抗体法染色DXMV细胞飞片，可在细胞浆内见到特异性荧光。以冠状病毒通用引物和犬冠状病毒特异性引物，可从大熊猫肝脏和不同代次的病毒细胞培养物中扩增出与预期值251bp和515bp大小相同的核苷酸片段。由此说明，该病毒为犬冠状病毒，大熊猫可被犬冠状病毒感染。     

制度给力成效显著——山东省精心打造农机购置补贴惠民工程

近几年来,山东省各级农机部门精心打造农机购置补贴惠民工程,坚持突出重点、规范操作、优化服务、强化监管,确保了补贴工作顺利高效运行,取得了农民得实惠、企业得效益、农机得发展、政府得民心的好效果.     

一种农业电网远程监测系统的设计

基于当前嵌入式的流行，设计了一套嵌入式网络服务器，对其设计方案及主要芯片端口和功能进行介绍，将其应用到农业电网远程监测系统中的网络通信，给出了系统的功能框图，所涉及到的技术有数据采集技术、实时网络通讯技术等等。     

A Comparative Study of Confined Concrete Models under Cyclic Loading

In this paper,four models are selected as the representatives and applied to nonlinear dynamic analysis program for space frames which is based on the beam-column elements using the finite element flexibility method and the fiber model.Park's test on combined axial and bending columns under cyclic loading are taken as the calibrations,comparisons to material and structural member level are drawn and the analytical results of various models are discussed.     

The Application of Pre-splitting Blasting to Excavation on High Slope in Soft Rock Section

Based on analyzing about blasting mode of slope in the construction of road, the paper shows that pre splitting blasting is mostly suitable in the section of severely weathering soft rock with developed joints and fractures, introduced the laying out about the hole of pre splitting blasting that can apply in the high slope of the expressway in the section, and designed the parameter. The practice shows that it gained favourable effect by this method.     

A Research on the Way of EMBET in the Tracking Systems'' Accuracy-analysis During Satellite Launch

In the past, the criterion for calibration has almost invariably been the stellar oriented ballistic camera,the method is a point to point comparison on the identical coordinate system. Deficiencies is follows: The request of weather is severe(cloudless night); The period is often long; the measure equipment of high precision can't be calibrated. While EMBET methods is used in calibration, it does not need criterion and it demands only that the measure elements is larger than 3,and the trajectory and many system errors of the measure elements can be simultaneously calculated with the measure data sequences. The error models of related equipments' measure elements and the EMBET of typical equipment group are given. The EMBET method is successfully used in Accuracy-analysis of Satellite Launch.     

Stability Ishikawa Iteration Procdeure for Strongly Pseudocontractive Operator and Strongly Accretive Operator Equations

By virtue of some analysis techniques,it is proved that certain ishikawa iteration procdeure with errors are stable with respect to strongly pseudocontractive operators in real Banach space. A related result deals with stable iteration procedures for solution of the nonlinear equation of strongly accretive operators. The results presented extend and improve the corresponding by Osilike,Zeng, Chidume, Deng, Liu,et al.     

地下滴灌灌水器出口正压试验研究

通过试验 ,研究了灌水器类型、流量、工作压力和土壤初始含水量对地下滴灌灌水器出口正压的影响规律。结果表明 :灌水器埋入土壤后 ,其出流由于受土壤等因素的限制 ,在灌水器出口处产生了一定的正压 ,该正压随着灌水历时的延长而增大。影响地埋灌水器出口正压的主要因素是灌水器的额定流量和土壤初始含水量     

秸秆覆盖对冬小麦耗水特征及水分生产率的影响

2季冬小麦试验结果表明 ,秸秆覆盖具有减少土壤棵间无效蒸发的作用 ,冬小麦返青前 1 m土层贮水量在砂壤土条件下秸秆覆盖处理比无覆盖处理增加 2 %～ 2 5 .8% ,粘壤土条件下增加 2 %～ 1 4.5 %。返青前秸秆覆盖处理比无覆盖处理的日耗水量普遍小 ,返青后情况则相反。秸秆覆盖能够促进作物生长 ,与无覆盖相比 ,提高水分生产效率 4.6%～ 2 5 .2 %。     

城市生活用水考核指标体系

提出了城市生活用水考核的指标体系。该考核体系分城市生活用水单元和城市总体生活用水两个类型，从生活用水、重复利用、排水、用水管理等4个方面对生活用水进行考核，从而全面的反映城市生活用水情况，对城市生活用水的规范化、科学化管理，促进节约用水将起到积极的作用。