Adipocyte-derived relaxing factor,a new vasodilative factor

Although our knowledge of ADRF (adipocyte-derived relaxing factor) is extremely limited,the little that is known has already revealed a promising future in this newly found factor.Firstly,it is secreted by adipose tissue,which is a abundantly and extensively distributed in human body and has become a hot spot for research work in recent years.Secondly,ADRF has shown a significant vasodilative action in a considerable number of experiments conducted on arteries of various sizes,from different body parts of different species of animals,including human.In this article,we introduce the development of ADRF research,sum up its known properties,including its Ca2+,protein tyrosine kinase,and protein kinase A dependent releasing,K+ channel mediated functioning,and interfered effect in different pathological models,and propose problems surrounding this factor and directions for future research work.     

松叶蜂病原微生物的研究进展

本文对我国松叶蜂几个危害区幼虫期或茧期的病原进行了调查，统计了松叶蜂类害虫的为害和病原的寄生情况。详细描述了国内外已分离的主要松叶蜂病毒各自超微结构的特征、所属类型、及其一系列研究近况。总结了近年来国内外利用松叶蜂病原微生物防治该类害虫取得的成果、目前所存在的问题。还就我国利用松叶蜂病原防治该虫进行了展望。     

大学毕业生就业质量的影响因素研究

"难就业、业就难、就业难"是普遍大学生的就业问题,时常有听到学生这样抱怨,相对于用人单位、企业也在抱怨"难招工、工难招、招工难",很多大学生就业质量较低.面对诸如此类看似矛盾的问题,文章通过对大学生就业现状的进一步分析,从企业与学生两个不同的方面进行分析影响就业质量的各种因素.     

基于校企合作的CDIO工程教育模式改革实践

针对CDIO工程教育体系滞后、校企合作深度不够等问题,探索基于校企合作的CDIO工程教育模式改革实践.分析现有校企合作人才培养存在的问题,提出实施CDIO工程教育,校企共建新体系;整合资源,营造工程实践教学环境;结合真实企业案例,奠定CDIO教育新基础;完善师资队伍建设,改革师资培养策略等改革,以提升学生综合职业能力和就业率.     

Cloning of Buffalo AQP9 Gene and Analysing its Expression in the Buffalo Tissues

Cloning buffalo AQP9 gene and analyzing its expression in buffalo tissues.A pair of primers was designed according to the released bovine AQP9 sequences in GenBank,which was used to clone buffalo AQP9 gene.The AQP9 gene was amplified by RT-PCR,whose nucleotide sequence and protein structure were analyzed by bioinformatics methods.The expression of AQP9 in buffalo tissues was assayed by Real-time quantitative PCR.The expression of AQP9 gene in buffalo ovary and testis tissue was detected by immunohistochemical staining method.The results showed that the cloned ORF length of buffalo AQP9 gene was 888 bp,which coded 295 amino acids.The results of multiple sequence comparison showed that the nucleotide sequence of buffalo AQP9 shared 99%,90%,97% and 88% homologeous compared with that of Bos taurus,Sus scrofa,Ovis ariessis and Homo sapiens,respectively,while shared 99%,86%,97%,83% homologeous for amino acids,respectively.Phylogenetic tree analysis indicated that AQP9 gene was highly conservative in the evolutionary process.Real-time quantitative PCR results showed that AQP9 gene expressed in buffalo liver,lung,brain,skin,testis and ovary tissues with different levels,had the most abundant expression in liver,followed by in skin and testis,less observed in lung and ovary.The results of immunohistochemical staining showed that the expression of AQP9 protein varied with the development of buffalo ovarian tissue,and gradually enhanced with follicle development.In testicular tissue,AQP9 protein expressed in spermatocyte and leydig cells of developmental stage testis.These results indicated that we had successfully cloned buffalo AQP9 gene sequences.The expression and its function of AQP9 in buffalo ovaries and testes might play an important role in follicle development and spermatogenesis.     

小体鲟卵黄雌性蛋白分离纯化及抗血清的研制

采用凝胶柱层析法,对小体鲟(Acipenser ruthenusLinnaeus)卵黄蛋白粗提液进行分离纯化。小体鲟卵黄蛋白粗提液的质量浓度为25.04 mg/mL,经Sephadex G-200层析后出现3个蛋白峰。用聚丙烯凝胶电泳、免疫印迹(Western-blotting)和免疫扩散等方法研究表明,小体鲟卵黄蛋白由分子量分别为132.1 kD、97.4 kD、85.9 kD、67.0 kD、59.2 kD、47.6 kD、30 kD、14.3 kD和12.8 kD的9个亚基组成;层析后峰B蛋白为卵黄雌性特异蛋白(yolk female specific protein,YFSP),是一种糖脂磷蛋白,具有雌性特异性和组织特异性,抗体具有种属特异性。Western-blotting结果表明,卵黄雌性特异蛋白(YFSP)与血清雌性蛋白(FSSP)免疫印迹图谱上都显示分子量为97.4 kD和30 kD的2条杂交带,可见两种蛋白在免疫原性上具有相似性,可以用卵黄雌性蛋白抗血清代替卵黄蛋白原抗血清对卵黄蛋白原进行检测     

聚合物发光二极管的研究进展

对共轭聚合物中进行电运输及电致发光器件－聚合物发光二极管的研究进展进行了综述。     

过饱和溶氧对大菱鲆生长及消化酶的影响

探讨工程化养殖中过饱和溶氧对大菱鲆生长的影响,通过充入液态氧与空气,使试验水体的溶氧分别达到过饱和溶氧水平(12±1)mg/L和(14±1)mg/L,正常溶氧水平(7.0±0.5)mg/L.结果显示,过饱和溶氧组的大菱鲆生长速度及饵料利用率明显高于正常溶氧组(p<0.05),而在过饱和溶氧条件下与正常溶氧条件下大菱鲆的丰满度无显著差异(P>0.05).对大菱鲆胃、肠道消化酶、脂肪酶与淀粉酶的分析发现,大菱鲆胃蛋白酶活性在过饱和溶氧条件下较正常溶氧有明显提高(p<0.05),而脂肪酶与淀粉酶的酶活力未得到显著性提高(p>0.05).     

轻卡汽车门锁故障分析与解决措施

门锁的定义为锁止车门的机构,主要包括锁体、挡块、内外操纵机构和内外锁止机构,是驾驶室的保护安全部件.本文介绍了轻卡汽车门锁常见故障.分析了门锁失效原因,提出了对轻卡汽车门锁常见问题的解决措施.     

小体鲟在静水池塘中生长观察

本试验利用西泉眼水库岸边静水池塘养殖开口进食已两周的小体鲟幼鱼,其体质量和体长分别为0.125±0.95g、2.98±1.72 cm;经过4周饲喂,其育成率达到70%以上;体长为6±2.98cm、体质量1.78±1.25g;体长、体质量增长分别为0.115cm/d、0.0595g/d.试验结果证实如配合采用调整养殖密度、配套养殖设施、清理天敌并在池塘周围建围栏、调节水质、越冬并搭配不同食性、不同水层的鱼种等措施,小体鲟是适于在北方静水池塘生长的.