Influence of Sini decoction on the proliferation and apoptosis of artery smooth muscle cells after balloon injury

AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs.     

Effects of Sini decoction on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion

AIM: To investigate the effect of Sini decoction (SND)on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion. METHODS: Thirty-two Sprague-Dawley rats of both sexes were randomly divided into 4 groups: (1) Control group in which sham operation was performed; (2) Model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours; (3) SND1 group in which SD (0.6 g/200 g rat) was given via stomach tube 3 d before intestinal I/R; (4) SND2 group in which SD (1.2 g/200 g rat)was given via stomach tube 3 d before intestinal I/R. A strip of small intestine was taken from distal end of ileum for electron microscopic examination. The two-dimensional structural parameters and three-dimensional structural parameters of mitochondria were calculated. RESULTS: (1)Morphological changes of small intestine: In control group, epithelial cells were orderly arranged, with normal mitochondria and intestinal villi. In model group, the gaps between epithelial cells widened. There were a lot of apoptotic cells. Microvilli were short and swelled. Mitochondria were swelled obviously with broken ridges. Endoplasmatic reticulum was severely dilated. In SND1 and SND2 groups, microvilli and epithelial cells were orderly arranged relatively, mitochondria was slightly swelled. (2) Structural parameters of mitochondria: In model group, there were the least mitochondria and the swelling of mitochondria was severe. In SND1 and SND2 groups, the mitochondria was more than that of model group and the swelling were slight. CONCLUSION: Sini decoction can protect small intestine from ischemia-reperfusion injury without dose-dependent effect.     

体外培养条件对延边黄牛受精卵体外发育的影响

从延边黄牛卵巢中采集未成熟的卵母细胞进行体外成熟培养、体外受精及受精卵体外培养。结果表明 :1将卵子用 2种不同的培养液进行体外成熟培养和受精卵体外培养 ,TCM199组的卵裂率 (5 6 .3% )极显著高于 D- PBS组(33.6 % ) (P<0 .0 1) ;TCM199组的囊胚发育率和孵化率 (15 .1%、13.7% )虽高于 D- PBS组 (10 .5 %、8.7% ) ,但 2个组之间无显著差异 (P>0 .0 5 )。2以 TCM199作为基础培养液 ,分别用含激素培养液和不含激素培养液进行成熟培养和受精卵体外培养 ,添加激素组的卵裂率、囊胚发育率及囊胚孵化率 (81.2 %、17.5 %、15 .3% )高于没有添加激素的对照组 (75 .8%、12 .1%、10 .5 % ) ,但 2个组之间无显著差异 (P>0 .0 5 )。 3体外受精卵与单层颗粒细胞共培养组的卵裂率、囊胚发育率及囊胚孵化率 (78.0 %、11.5 %、9.9% )显著高于非共培养组 (6 8.1%、5 .4 %、3.6 % ) (P<0 .0 5 )。     

RT-PCR技术检测猪瘟病毒的应用研究

本试验应用反转录-聚合酶链式反应(RT-PCR)对猪瘟进行诊断应用研究.应用RT-PCR对来自广西不同地区的135份疑似猪瘟病料进行检测,84份诊断为阳性,阳性率62.2%.从百色、柳州地区等采集的健康猪扁桃体和淋巴结共276份,经RT-PCR检测,37份为阳性,阳性率为13.4%.其中健康猪扁桃体带毒较高,246份扁桃体中有35份阳性,占14.2%.采自柳州健康猪的26份淋巴结材料全为阴性,只有邕宁县的1份健猪淋巴结阳性.结果表明,RT-PCR技术可应用于猪瘟的临床诊断.     

猪原始生殖嵴细胞(PGCs)建系因素的研究

从五指山猪(WSZP)近交系第8～13代培育群中，先后选用21头5～10月龄青年母猪，分别于授精后25～30d采集胎儿106个，进行原始生殖嵴(PGCs)细胞分离、培养等建系技术研究。以DMEM F10(1:1)为基础培养液，按添加或不添加生长因子，将培养液分为A、B、C3种，并以STO细胞作饲养层，在38℃、5.0％CO2和湿润的气相中进行培养建系。结果获得胚胎生殖嵴细胞(EG)细胞系6个细胞株，其中1个EG细胞株传至11代、2个传至5代、1个传至4代、2个传至3代冻存。并进行了AKP染色、体外分化、冷冻-解冻复苏和嵌合体制作等鉴定研究。研究发现：不同胚龄对EG细胞建系具有一定影响，不同培养液对EG细胞建系效果不同，STO细胞饲养层的质量是建株、传代、冷冻-解冻复苏的关键因素之一。EG细胞系的初步建立，为今后筛选进入种系的EG细胞系、实施体外基因操作提供了可能。     

浙江地区不同品种紫花苜蓿不同刈割期二茬性状表现

对不同秋眠级别的紫花苜蓿Medicago sativa品种进行适应性试验,研究不同品种不同刈割期株高、分蘖数、根系、茎叶比及生物学产量间的变化,为生产推广提供指导.     

科尔沁草原主要草地植物传播生物学简析

本研究论述了科尔沁草原主要草地植物的传播生物学特性.在141种植物中,44种以种子作为传播体,97种以果实作为传播体;传播体重量< 0.1 mg的植物12种,重量0.1～0.999 9 mg的植物61种,重量1～9.999 9 mg的植物55种,重量10～99.999 9 mg的植物12种,重量100～999.999 9 mg的植物1种;具有典型聚合传播体的植物4种;具有典型多态传播体的植物1种;传播体具附属物的植物66种(占总数的46%),其中19种带冠毛,10种带绢毛或丝毛,15种带芒或刚毛,6种带翅,6种带钩或刺,10种带其他附属物(花柱、花萼、结块和喙等).在124种植物中,11种植物(7种蒿属植物、2种车前、百里香1种和野亚麻1种)种子具有粘液,其种子或果实的干重＜1 mg.已发现23种植物具有明显的植冠种子库(植冠种子库种子达到总结种量的5%).8种植物植冠种子库种子超过结种量的30%,7种植物达到结种量的20%～30%,8种植物少于结种量的20%.具有植冠种子库的植物以菊科(尤其蒿属)、禾本科和藜科植物为多.具有较强或相对较强沙生适应性的植物多数具有植冠种子库.探讨了传播体类型和重量、利于(或阻滞)传播的传播体特征、传播体多态性与传播适应的多面性、延缓传播与植冠种子库等方面的问题.     

鸡AMPD1基因PCR-SSCP分析与相关性状的研究

5′-磷酸腺苷脱氨酶1(AMPD1)是嘌呤代谢中的一种重要酶类,它的功能是催化AMP(一磷酸腺苷)脱氨,生成肌苷酸(IMP),从而影响肉质风味。试验采用单链构象多态性(PCR-SSCP)技术,以隐性白羽肉鸡、白来航蛋鸡和两个地方品种(北京油鸡、三黄胡须鸡)纯系鸡为试验材料,对AMPD1基因进行SNPs检测和基因类型判别。卡方检验结果表明: 除三黄胡须鸡和隐性白羽肉鸡、北京油鸡和白来航鸡间差异不显著(P>0.05)外,其他各品种间差异极显著(P<0.01)。AMPD1基因多态性与北京油鸡生产和屠体性状(活重、胸肌率、肌苷酸含量等)相关分析结果表明:肌苷酸含量在三种基因型间差异显著(P<0.05)。初步推断AMPD1可能为影响鸡肉中肌苷酸生成的主效基因或与主效基因相连锁。     

赖氨酸采食量对早期断奶仔猪生长发育及胰腺内分泌机能的影响

将55头21日龄断奶的雄性仔猪随机分为5组，各组仔猪饲喂赖氨酸含量为0．60％、0．80％、1．00％、1．20％和1．40％的试验日粮21d。42日龄结束饲养试验时进行葡萄糖灌注试验，观察赖氨酸采食量对早期断奶仔猪生长发育和胰腺内分泌的影响。结果表明：早期断奶仔猪体增重与赖氨酸采食量为强正相关，根椐体增重指标，21～42日龄阶段，仔猪的赖氨酸采食量不应低于3．16g／d，日粮赖氨酸含量不应低于1.40％。赖氨酸严重缺乏(采食量小于1．45g／d)时，胰岛素的分泌量下降，对高血糖应激的反应能力降低。     

黄羽肉鸡育肥期维生素A、E适宜添加量研究

选体重和脚胫颜色度接近的56日龄黄羽肉鸡共960只,随机分为16个处理组,每处理60只鸡。采用双因子4×4水平的随机试验设计,4个维生素A添加水平(0、5000、10000、20000IU/kg)和4个维生素E添加水平(0、30、60、120IU/kg)。结果表明:在维生素A0～10000IU/kg、维生素E0～60IU/kg范围内,黄羽肉鸡的日增重和饲料报酬逐渐提高;日粮维生素A添加量超过10000IU/kg,血浆中维生素E含量有下降趋势。高水平维生素A(20000IU/kg),升高血浆TBARS值,大剂量维生素E(120IU/kg),提高机体脂质稳定性。日粮添加20000IU/kg维生素A对色素的沉积有抑制作用,随维生素E水平的上升,皮肤的颜色指数呈现上升趋势。维生素A和E含量及其互作对血清新城疫抗体效价有显著影响。综合考虑,黄羽肉鸡育肥期维生素A、E的适宜添加量分别为5000～10000IU/kg,30～60IU/kg。