苦瓜强雌系Q03的选育及利用研究

对回交转育的中间材料定向选择,选育出了草白色果皮的强雌系Q03苦瓜,其雌花率90%以上,且雌性稳定,以其作母本配制的川苦77,早期产量和总产量杂种优势明显,与适宜的亲本配组表现出正向的强雌性杂种优势。同时便于杂交种子生产。     

生物药剂防治苹果树腐烂病的田间试验

河南省自1987年开始在孟县、卢氏等县265亩苹果园中进行生物药剂治疗苹果树腐烂病的试验示范。以刮皮法和划刻法用农抗120、S-921和843康复剂涂抹病疤,一年后在发病季节调查。以刮皮法用农抗120处理的病疤,治愈率达100％,无一复发。以划刻法用农抗120处理的病疤治愈率为78-90％,以S-921和843康复剂处理的治疗效果达100％。作者认为这些生物药剂可取代福美砷治疗苹果树腐烂病。     

鸡CD4蛋白基因的克隆及其mRNA在淋巴器官中的表达

从鸡胸腺、脾脏、哈德氏腺、外周血液和法氏囊等免疫器官中分离淋巴细胞,应用RT-PCR对这些免疫器官中鸡CD4分子mRNA的表达进行了研究。同时,用RT-PCR对鸡脾淋巴细胞CD4分子cDNA进行了克隆和序列测定。结果表明,在上述器官中,法氏囊淋巴细胞不表达鸡CD4分子mRNA,其他器官中均有表达。通过序列分析表明,本试验扩增得到的基因为编码鸡CD4分子的基因。     

农艺措施对大豆籽粒蛋白质和脂肪含量的影响

探讨了栽培密度、撒可富专用肥、思德富多元长效肥等农艺措施对大豆籽粒蛋白质和脂肪含量的影响。结果表明 ,密度对蛋白质含量、脂肪含量和蛋白脂肪总含量的影响不大。在各种栽培密度中 ,品种九三 97- 6 0是在 30万株 /hm2 时籽粒的蛋白质含量、脂肪含量和蛋白脂肪总含量均达到最高值 ,而对于品种九三 96 - 12 7来说 ,是在 2 5万株 /hm2 时三者达到最高。并且 ,蛋白质含量、脂肪含量及蛋白脂肪总含量对栽培密度敏感程度因品种而异。撒可富专用肥和思德富多元长效肥均使蛋白质含量增加 ,使脂肪含量降低。施用撒可富专用肥后 ,蛋白脂肪总含量变化因处理而异。施用思德富后 ,蛋白脂肪总含量有所增加。     

Alteration of serum interleukin-6 and tumor necrosis factor-α after ischemic stimulation of coronary artery in PTCA

AIM: Inflammatory responses play an important role in the post- percutaneous transluminal coronary angioplasty (PTCA) restenosis and has been demonstrated occuring immediately after PTCA. Interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) are the main inflammatory cytokines. We try to compare the changes in interleukin-6(IL-6) and TNF-α after PTCA in the patients with and without collateral circulation to probe into the pathogenesis of early inflammatory response. METHODS: The extent of myocardial ischemia induced by balloon inflation was quantified by a scoring system referring to the Leaman coronary score. The IL-6、TNF-α levels of coronary heart disease group and control group before and after PTCA are calculated. RESULTS: The concentrations of IL-6 and TNF-α were (9.592±1.847) ng/L and (26.959±1.967) ng/L, respectively, and were significantly increased 4 hours after PTCA. CONCLUSION: IL-6 and TNF-α are sensitive indicators of the early inflammatory response after PTCA. Ischemia scores reflected the extent of ischemia reperfusion injury during PTCA. Collateral circulation decreased the early inflammatory response after PTCA.     

中药甜菜碱复方对高温环境下三黄鸡某些血液生化指标的影响

试验选取15日龄的三黄鸡288只,随机分为8组。高温对照组仅喂基础日粮,中药Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.4%、0.8%、1.2%的中药,中药-甜菜碱Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.4%中药-0.2%甜菜碱、0.8%中药-0.2%甜菜碱、1.2%中药-0.2%甜菜碱,甜菜碱组在基础日粮中添加0.2%甜菜碱,探讨中药甜菜碱复方对高温环境下三黄鸡血液生化指标的影响。结果:①42日龄,在日粮中添加1.2%中药-0.2%甜菜碱试验组0.8%中药-0.2%甜菜碱试验组母鸡血清血糖含量分别比高温对照组高26.5%(P<0.05)、19.4%(P<0.05);在日粮中添加1.2%中药-0.2%甜菜碱试验组公鸡血清血糖含量比高温对照组高24.3%(P<0.05);②42日龄,在日粮中添加1.2%中药-0.2%甜菜碱试验组母鸡血清白蛋白含量比高温对照组高34.5%(P<0.05);在日粮中添加1.2%中药-0.2%甜菜碱试验组公鸡血清白蛋白含量比高温对照组高18.8%(P<0.05)。试验结果表明,中药甜菜碱复方能提高高温环境下三黄鸡血清总蛋白、白蛋白、血糖的含量,以在日粮中添加1.2%中药-0.2%甜菜碱复方效果最明显。     

甲壳素、壳聚糖对家禽生产性能及脂肪代谢的影响

本文就甲壳素、壳聚糖的发现、来源、制备、结构和性质;甲壳素、壳聚糖对家禽生产性能及脂肪代谢的影响;甲壳素和壳聚糖的降脂机理及今后的研究发展方向进行综述。     

胸膜肺炎放线杆菌ApxⅡ毒素结构基因的原核表达以及重组蛋白的免疫原性分析

猪传染性胸膜肺炎（Porcine infectious pleuropneumonia）是由胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae，APP）引起的猪的一种呼吸道疾病。本实验以APP血清7型的一株现地分离株L25—4株为研究对象，对其分泌的ApxⅡ毒素的结构基因apxⅡA进行了全基因克隆和测序，并利用原核表达载体pGEX-6P-1在E．coli中进行了原核表达。表达产物以包涵体形式存在，包涵体蛋白经过洗涤后作为抗原用于Western—blotting免疫印迹实验，结果表明重组的ApxⅡA蛋白具有良好的免疫原性。     

0.13%百瓒防蛀气雾剂毒理学研究

对0.13%百瓒防蛀气雾剂及其主要有效成分辛硫磷进行了毒理学研究。结果表明:急性吸入毒性(LC50):雌、雄小鼠均大于2150mg/m3;急性经口毒性(LD50):小鼠为9 210mg/kg;皮肤刺激实验:无刺激作用;皮肤变态反应:致敏率为“0”;眼刺激试验:无刺激性;辛硫磷原药(85%)对大鼠28d亚急性吸入毒性:对雄、雌性大鼠无作用浓度(NOAEL)均为10mg/m3。     

Effect of basil polysaccharide on rat model bearing NuTu-19 cells

AIM：To study the effect of basil polysaccharide on the rat model bearing NuTu-19 cells.METHODS：The rat model bearing NuTu-19 cells was treated with basil polysaccharide.The changes of the tumor cell biology and histopathology were observed.Total RNA was isolated from the tumor tissue.Hybridization assay was performed using gene expression microarray.The results were analyzed by photo disposal software-GenePix Pro3.0.RESULTS：Compared with control,the amounts of ascites in the rats treated with basil polysaccharide were decreased (P<0.05) and the scores of abdominal carcinoma metastasis were changed significantly (P<0.01).268 differentially expressed genes were found in the tumor specimen from the rats exposed to basil polysaccharide.CONCLUSION：Basil polysaccharide may be a new anti-tumor drug and it has multi-targets.Gene expression microarray has huge advantage and value in the study of the drug target.