HACCP体系在出口兔肉检验检疫备案养殖场中的应用

输欧盟兔肉一直是出口敏感商品之一,多次因药物残留等问题被欧盟通报,严重影响了我国兔业的出口,而问题的根源主要在肉兔的养殖过程。本文就肉兔养殖过程中建立和应用HACCP体系的可行性和必要性进行探讨,对养殖过程中的安全危害进行分析,确定关键控制点,提出控制措施,从源头保证输欧兔肉产品的质量安全卫生。     

白水生姜组培脱毒及离体快繁体系建立研究

以白水生姜茎尖为材料进行组培脱毒及离体快繁体系建立研究,结果表明,姜块催芽并在40～45℃光照箱中热处理30 d,再结合微茎尖（0.1～0.3 mm）分生组织培养,能有效脱除白水生姜烟草花叶病毒（TMV）和黄瓜花叶病毒（CMV）。以MS为基本培养基,添加6-BA 2.0 mg/L、NAA 0.1 mg/L有利于茎尖诱导分化;最适试管苗增殖培养基为MS＋6-BA 3.0 mg/L＋NAA 0.03 mg/L,增殖系数达7倍,丛芽叶绿、壮,长势好;生根培养基以MS＋NAA 0.5 mg/L＋IBA0.1 mg/L为宜,生根率达100%,纤维根多。     

基于Web Service的城市绿地有害生物PDA实时监控系统的开发与应用

基于Web Service技术,结合个人数字助理(personal digital assistant,PDA)的使用,开发了城市绿地有害生物实时监控系统,从而解决城市绿地有害生物预警监测中信息交流不畅和共享困难的问题.调查人员手持PDA进行实地信息采集,通过无线网络即时传输;系统管理平台实时接收调查结果,经分析处理后进行信息发布.该系统应用于上海世博园区绿化植物有害生物实时监控,共收集有害生物信息153次,发布预警信息16期.结果表明,基于Web Service的城市绿地有害生物PDA实时监控系统能即时获取并发布信息,实现对有害生物的实时化、信息化监控.     

Occurrence of Honeysuckle Yellow Vein Virus (HYVV) containing a monopartite DNA-A genome in Korea

Two newly emerged begomoviruses were isolated from naturally infected tomato (Solanum lycopersicum) plants grown in greenhouses at Jeju Island and Dangjin in Korea and their genomes were characterized. These viruses-infected plants had very small leaves that curled upward, yellow margins and a leathery appearance, and a bushy and stunted appearance with short internodes. Nucleotide (nt) sequence analysis of their genomes showed that they have a DNA-A component of a monopartite begomovirus. Their genomes comprised 2763 and 2764 nucleotides with six open reading frames. The results of nt sequence similarity analysis of DNA-A genome between the two Korean isolates and isolates of Tobacco leaf curl Japan virus (TbLCJV), Honeysuckle yellow vein virus (HYVV), Honeysuckle yellow vein mosaic virus (HYVMV), and Eupatorium yellow vein virus in Japan (EpYVV) showed that they are likely similar to HYVV-[Masuda] (89.4–92.8% nt identity). Consequently, we tentatively propose the two isolates’ names as HYVV-Jeju and -DJ according to the ICTV geminivirus rules. Phylogenetic relationship analysis of 33 DNA-A genome sequences using PAUP* 4.0b10 and MrBayes revealed that HYVV-Jeju and -DJ belong to the Far East Asian begomovirus species complex. Within the Far East Asian begomovirus species complex, HYVV-Jeju and -DJ are distantly related to EpYVV, HYVMV, and TbLCJV groups. Based on the presence of a recombination fragment spanning the C3 ORF, a recombinant origin was suggested for both HYVV-Jeju and –DJ, with parents close to Japanese isolates HYVMV-[SP1:00] and Eupatorium yellow vein virus (EpYVV)-[Suya]. In addition, the presence of a further recombination fragment spanning the IR suggested the parents of HYVV-DJ were close to HYVV-Jeju and EpYVV-[Suya].     

稀土元素在玉米体内的吸收及富集状况研究

利用仪器中子活化分析测定玉米不同生长期不同部位的单一稀土含量。结果表明：稀土元素在玉米植株体内主要是向结实及生命活动旺盛部位运转，玉米植株在生长各阶段对元素Ｌａ，Ｃｅ，Ｎｄ的吸收最为敏感。这３种元素可能是作物体内活性物质的主要激活剂。     

上海市老港垃圾填埋场潮滩重金属污染及评价研究

以上海市老港垃圾填埋场潮滩为例,研究了沉积物中重金属的分布特征,并采用化学法和潜在生态危害指数法评价了潮滩重金属的污染状况及其潜在生态危害,以期为保护潮滩环境以及利用潮滩进行垃圾渗滤液的净化提供科学依据。结果表明,受垃圾渗滤液排放和生物活动影响,老港潮滩表层沉积物中重金属(除Cu外)的含量在向海向上均表现为:高潮滩>低潮滩>中潮滩;Cr和Zn的含量在氧化池排污口处明显高于其它排污口,而Pb的含量在自然排污口处最高;老港潮滩沉积物受到一定程度的重金属污染,以Zn的污染最为严重,由这4种元素造成的潜在生态危害是中等的,其中,Cu和Pb的毒性贡献最大。     

温室发展进程初释

保护地栽培(设施园艺)做为一种农业生产方式的出现和发展,在古代都是为宫廷和贵族服务。西欧是从古罗马帝国的第五代皇帝尼禄(Nero)时期(公元54—68年)掘坑后覆盖云母或滑石板片进行保护地栽培。中国确切的记载是在公元前33年汉朝时期,采取燃柴草加温的方法在室内进行蔬菜生产。由于封建迷信思想(认为食之有害)的影响长期以来没有得到发展。到唐朝(公元7世纪)又有利用温泉为宫廷种植瓜类的记载。至于北京温室的历史可以追溯到明朝中叶(公元16世纪)。许多栽培技术究竟从什么时候开始至今无法肯定,但一定比文字记载要早,这是完全可以肯定的。     

Partial replacement of Fe(o,o‐EDDHA) by humic substances for Fe nutrition and fruit quality of citrus

The most widely used Iron (Fe) fertilizer in calcareous soils is the synthetic chelate Fe(o,o‐EDDHA). However, humic substances are occasionally combined with Fe chelates in drip irrigation systems in order to lower costs. We investigated the effect of various mixtures of Fe(o,o‐EDDHA) and a commercially available humic substance on Fe availability in a calcareous soil from Murcia, Spain (in vitro experiment) and on leaf Fe content and fruit‐quality attributes of Citrus macrophylla (field experiment). In the in vitro experiment, a calcareous soil was incubated for 15 d with solutions of sole Fe(o,o‐EDDHA) and humic substance and of a mixture of humic substance and Fe(o,o‐EDDHA) to determine the dynamics of available Fe. While the mixture did not significantly increase the available soil Fe, it did decrease the rate of Fe retention in the surface soil compared to sole Fe(o,o‐EDDHA). In the field experiment, the substitution in the application solution of 67% of Fe(o,o‐EDDHA) by commercial humic substance increased leaf P in lemon trees from 0.19% with sole Fe(o,o‐EDDHA) to 0.30% and leaf Fe from 94 mg kg^–1 to 115 mg kg^–1. Some quality parameters like vitamin C content and peel thickness were also improved with a partial substitution of Fe(o,o‐EDDHA) by humic substances. We conclude that a partial substitution of commercial Fe chelates by humic substance can improve crop Fe uptake and may thus be economically attractive. The underlying physiological mechanisms and ecological implications require further studies.     

我国东北地区土壤中的硒

硒是人和其它动物必需的微量元素,土壤和植物中硒含量过低,可引起牲畜的白肌病,如果土壤和植物中硒含量过高,又会引起牲畜的硒中毒现象。在国外,硒与动物健康关系的研究工作比较多^[7,5]。     

河南省典型淋溶土土系划分研究

在河南省选取8个典型土壤剖面,对其进行景观特征、剖面形态学特征的描述及其理化性质的分析,并确定其在土壤系统分类(Chinese Soil Taxonomy)中的归属,结果表明,供试土壤归属于壤质混合型石灰性温性普通简育干润淋溶土、壤质混合型非酸性温性斑纹简育湿润淋溶土、黏壤质混合型温性斑纹钙积干润淋溶土、黏壤质混合型温性普通钙积干润淋溶土、壤质混合型石灰性温性普通简育湿润淋溶土和壤质混合型非酸性温性普通简育湿润淋溶土等6个土族;并在此土族基础上,根据土系划分规则和河南地区淋溶土诊断特征及构型特征,尝试提出"土系对比检索逻辑图"以阐述土系参比划分的逻辑过程。通过对比,可将8个典型土壤剖面划分为7个土系:汤阴系、汲水系、神沟系、尚庄系、侯集系、枣林系和尚店系。