肥胖基因研究进展

与肥胖有关的基因已发现很多，但ob基因是研究的重点，其表达产物Leptin的作用机制已有较深入的了解。本文主要介绍了ob基因及其产物Leptin的作用机制、生物学效应、Leptin抵抗现象，以及其它与肥胖有关的基因和蛋白。     

Identification of a Long Rice Spikelet Mutant

A spontaneously occurring rice (Oryza sativa L. ) mutant, characterized by homeotic conversion in glumes and stamens, was found in the progeny of a cross. The mutant showed long glumes and glumaceous lodicules and morphological transformation of stamens into pistils. Mutant florets consisted of 1 to 3 completely developed pistils, some pistilloid stamens with filaments, but tipped by bulged tissue and 0 to 3 stigmas. It seens that the mutant phenotype of the homeotic conversions in glumes and stamens is similar to that of the B loss-of-function mutants in Arabidopsis and Antirrhinum. The mutant is controlled by a single recessive gene as a segregation ratio of 3:1 (wild type to mutant plants) was observed in the F2 generation.     

黑杨无性系日CO2固定总量的测定及与苗木生产潜势的相关性研究

通过对不同基因型黑杨无性系植株净光合速率日变化曲线与光合主体叶面积在时间和光强上进行累计积分来计算植株日CO2固定总量P。由于净光合速率日变化曲线不易准确测得，故以光响应曲线和光强的日变化来推测净光合速率日变化。对P值和生物量的相关性研究表明，二者的相关性很高。且认为可以用计算出的P值作为评估苗木生产潜势的指标之一，这为育种中生产力的早期选择提供了有力的证据。     

高寒牧区牧鸡鸭治蝗效果试验

在青海湖北岸高寒牧区利用人工饲养的鸡鸭进行放牧防治草原蝗虫。结果表明,在60d的防治期内,牧鸡鸭平均灭治效果达91.4%,为人工利用天敌治虫技术在高寒牧区的应用提供了依据。     

极早熟大白菜新品种豫新50的选育

采用游离小孢子培养双单倍体技术快速育成早熟大白菜杂交一代新品种豫新50。结果表明:豫新50为极早熟耐热品种,从播种到采收50-56d,高抗病毒病和软腐病,抗霜霉病;平均667m2产净菜2716.87kg,株形紧凑,适宜密植;叶球矮桩叠抱,整齐度高,商品性好;质地柔嫩,风味佳,每100g鲜样含粗蛋白质1.19g,粗纤维0.40g,维生素C20.8mg,可溶性糖2.80g。     

猪生殖与呼吸综合征病毒M基因和N基因的表达

将猪生殖与呼吸综合征病毒（PRRSV）的M基因和N基因从重组质粒pMD18-T—M—N中亚克隆至pBV220原核表达栽体上，成功构建了重组表达质粒pBVM—N。将pBVM—N转化大肠埃希氏菌DH5α感受态细胞．重组菌经温度敏感诱导表达，其细菌裂解物经SDS—PAGE可检测到分子质量约为19ku的目的蛋白，与M基因表达产物一致；经蛋白质分析软件Bandscan分析，其表达量可达19．1％；Western-blotting分析表明，该重组蛋白可被兔抗PRRSV血清所识别，与预期的M基因表达产物相一致，而N基因未获表达。     

鸡球虫病免疫学研究进展

鸡球虫病是严重危害养禽业的一种寄生虫病。一直以来,鸡球虫病主要依靠药物来进行防治。但是,随着球虫抗药性问题的日益严重,抗球虫新药开发困难及人们对食品安全的关注,药物在球虫病控制中的作用越来越受到限制,因而用免疫方法来控制鸡球虫病日益受到重视,并有望成为主要的技术手段。目前鸡球虫免疫学研究主要集中在鸡球虫免疫原性、宿主免疫应答及寻找有效的鸡球虫保护性抗原等方面。近年来随着分子生物学和现代生物技术的发展,鸡球虫基因工程疫苗以其独特的优势显示其在未来球虫病控制中的诱人前景,并可能取代传统疫苗而成为鸡球虫病防治的主要手段。     

菌根与植被恢复

菌根不仅可促进植物的营养吸收、生长发育、抗病、抗逆,而且在保持良好的土壤结构、控制水土流失方面具有直接的作用。在我国西北许多地区,菌根菌与植物间的共生关系已被中断,要恢复该地区的植被,治本的方法应是重建植物与菌根菌的共生关系,形成健康的生态系统。文中还提出了菌根技术在植被恢复中的应用策略。     

乌鲁木齐区域水资源利用中的生态经济问题及其调控对策

本文在考察乌鲁木齐区域水资源利用现状的基础上 ,从生态经济学的角度总结和分析了该区域水资源利用中存在的问题 ;针对问题 ,提出了相应的调控对策。     

Effect of fibronectin on proliferation and collagen synthesis in cardiac fibroblasts from spontaneously hypertensive rats

AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFb_SHR) and WKY (CFb_WKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and[³H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm² of FN. Collagen synthesis was determined by [³H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×10⁴ cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in [³H]-proline incorporation in both CFb_SHR and CFb_WKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY.