早熟金针菇新品种G1的杂交选育

以金针菇菌株F3-31和FM-83为亲本,经过杂交选育出优良白色金针菇菌株G1.以菌丝生长速度、生长势、产量、子实体农艺性状及栽培周期等为指标,对金针菇菌株G1与其亲本进行比较.试验结果表明,G1菌丝生长速度快且生长势旺盛;平均单瓶产量达216 g,比亲本分别增产54 g、20 g,生物转化率也比亲本菌株高,达到72%;栽培周期43 d,比亲本分别缩短1 d、6 d;子实体各项农艺性状优良,是适于金针菇工厂化栽培的早熟白色优良菌株.     

西瓜EST-SSR分子标记的筛选

利用国际共享获得的820条EST序列,自主设计出79对EST-SSR引物,其中有32对引物经过PCR扩增出稳定清晰的电泳条带,可以作为西瓜和相关物种的新的EST-SSR分子标记。     

利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST

 以辣椒(Capsicum annuum L. ) 细胞质雄性不育系23A、121A, 和其相应的近等基因恢复系23C、121C为试验材料, 利用抑制消减杂交( SSH) 技术成功构建了CMS恢复基因诱导表达的消减cDNA文库。结合高密度点阵膜杂交差异筛选, 获得了282个阳性克隆。通过测序, 除去重复序列共得到175个Unique ESTs。在GenBank上进行BLAST分析, 55个EST片段未找到对应的同源序列, 可能代表了新基因;120个EST片段找到了对应的同源序列, 包括103个已知功能基因和17个未知功能基因。按照MIPS功能分类法, 将其分为14个功能组, 涉及代谢、胁迫应答、蛋白活性、转录因子、信号转导等多方面的功能。     

Relationship between zinc and zinc transporter expression in human lung cancer cells

AIM: To study the changes of zinc transporter gene expression in A-549 cell line exposed to ZnCl2 and N,N,N’,N’-tetrakis 2-pyridylmethyl ethylenediamine (TPEN).METHODS: Human lung cancer cell line A-549 was exposed to different concentrations of ZnCl2 (0, 50, 100, 150, 200 μmol/L) and TPEN (0, 5, 10, 15, 20 μmol/L), respectively. Twelve hours later, the cell viability was measured by MTT (methyl thiazolyl tetrazolium) and levels of zinc transporter mRNA was detected by RT-PCR. Zinquin was used to estimate the intracellular zinc concentrations.RESULTS: A-549 cell viability rate was significantly decreased when exposed to ZnCl2 at concentrations of 150 and 200 μmol/L, and to TPEN. The intracellular zinc concentration was significantly increased when exposed to ZnCl2 and decreased when exposed to TPEN. Zinc transporter (ZnT-1) mRNA level was increased along with the increase in the concentration of ZnCl2 but decreased when exposed to TPEN. The expressions of ZIP-1 and ZIP-10 (Zrt-and Irt-like protein) were increased along with the increase in the concentration of TPEN but decreased when exposed to ZnCl2.CONCLUSION: ZnT-1 expression is induced by zinc supplement. ZIP-1 and ZIP-10 expressions are induced by zinc deficiency and repressed by zinc supplement.     

Effect of inhibiting nuclear factor-kappa B on TNF-α-induced expression of angiotensinogen and production of angiotensinⅡ in glomerular mesangial cells

AIM: To study the effect of inhibiting nuclear factor-kappa B (NF-κB) activity on the expression of angiotensinogen (AGT) and the production of angiotensinⅡ (AngⅡ) induced by tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (MCs) of SD rats. METHODS: The MCs of SD rats were isolated and divided into three groups as follows: control; MCs treated with TNF-α, and the MCs treated with TNF-α + pyrrolidinedithiocarbamate (PDTC). The activity of nuclear factor-kappa B was measured by electrophoretic mobility shift assay. The expression of AGT was determined by RT-PCR for mRNA and Western blotting for protein. The concentration of angiotensinⅡ in supernatant was measured by RIA. RESULTS: The NF-κB activity in the MCs treated with TNF-α (20.67±9.14)×102 μg/cell was significantly higher than that in control cells ［(8.25±4.35)×102 μg/cell, P<0.01］ and the MCs treated with TNF-α+PDTC ［(7.20±4.57)×102 μg/cell, P<0.01］, and no significant difference was found between control and the MCs treated with TNF-α＋PDTC (P>0.05). The AGT mRNA level in the MCs treated with TNF-α (0.27±0.05) was higher than that in the control cells (0.20±0.05, P<0.05), and no significant difference was observed when compared with that in the MCs treated with TNF-α＋PDTC (0.22±0.06, P>0.05). The expression of AGT protein in the MCs treated with TNF-α (0.60±0.19) μg/cell was higher than that in the control ［(0.37±0.15)μg/cell, P＜0.05］ and the MCs treated with TNF-α＋PDTC ［(0.37±0.17)μg/cell, P<0.05］, and no significance was found between the MCs treated TNF-α＋PDTC and the control (P>0.05). The AngⅡ level in supernatant of cultured MCs treated with TNF-α ［(9.73±2.38)×10-5 ng·L-1/cell］ was significantly higher than that in the control ［(7.50±1.51)×10-5 ng·L-1/cell, P<0.05］ and in the MCs treated with TNF-α＋PDTC ［(6.94±1.46)×10-5 ng·L-1/cell, P<0.05］, however, the difference between the MCs treated with TNF-α＋PDTC and the control was of no significance (P>0.05). CONCLUSION: TNF-α activates the NF-κB in glomerular MCs, induces the AGT expression and the production of AngⅡ. Inhibition of NF-κB decreases the AGT expression and the production of AngⅡ. Therefore, the effects of TNF-α on AGT and AngⅡ may be mediated by NF-κB.     

一例奶牛皱胃溃疡伴低血钾综合征的临床治疗

2005年12月郑州牧业工程高等专科学校附属兽医院接诊了1例皱胃溃疡伴低血钾综合征的奶牛，该牛同时患有低血钙、酮病等。病情发展中患牛在血钾含量为2.28 mmol/L时四肢无力，卧地不起，通过补钾及综合治疗血钾升至3.45 mmol/L时又能自主站立，对皱胃溃疡采取消炎、抑制胃酸分泌、收敛止血等方法进行治疗。     

山葡萄‘双红’、‘双优’在戈壁滩上栽培的表现

报道我国西部栽培的‘双红’和‘双优’两个山葡萄品种不埋土能正常越冬，调查、测定和分析这两个山葡萄品种在冬季寒冷、冬春又极干旱的西部戈壁滩上栽培的生产表现。两个山葡萄品种根系耐低温能力比欧亚种酿酒葡萄品种强，枝条保水能力更强，因此，在干旱荒漠的戈壁石砾地上不埋土能安全越冬和抗御春日多风和干旱。一年生枝条长势好，花芽能正常形成，但在开花季节花序干枯和脱落现象严重。‘双红’、‘双优’山葡萄表现生产性能差、果穗少、果穗小、穗重轻、果粒小，故产量低，仅为原产地的1／3，但其品质好，可溶性固形物高出3．9～4．6个百分点。为使‘双红’、‘双优’在河西戈壁滩上栽培，既不埋土又有较高的产量，提出一些解决措施。     

玉米苗期耐碱性鉴定方法研究

在三叶一心期,用一定浓度梯度的Na2CO3溶液（5～25mmol&#183;L^-1）对玉米幼苗进行胁迫处理,观察其生长情况,7d后测定地上部分株高、鲜重、干重等生理指标。研究表明,各项生理指标均随Na2CO3溶液浓度的增加而降低,并在各碱浓度间、自交系间达极显著差异。综合苗情及各生理指标的变化,初步确定可以将25mmol&#183;L^-1 Na2CO3溶液作为玉米苗期耐碱性筛选的理想浓度,将该浓度下的苗情及株高、鲜重、干重及含水量变化率作为玉米苗期耐碱性筛选的鉴定指标。     

大红犀猎蝽对草地贪夜蛾3龄幼虫捕食功能反应

为探明天敌昆虫大红犀猎蝽Sycanus falleni对草地贪夜蛾Spodoptera frugiperda幼虫的捕食潜力,在室内条件下测定大红犀猎蝽3龄若虫和成虫对草地贪夜蛾3龄幼虫的捕食功能反应、搜寻效应,以及不同自身密度对捕食草地贪夜蛾的干扰反应。结果表明:大红犀猎蝽3龄若虫和成虫对草地贪夜蛾3龄幼虫的捕食功能反应符合Holling II模型;其中大红犀猎蝽3龄若虫对草地贪夜蛾3龄幼虫的日最大捕食量、瞬时攻击率和处理时间分别为4.049头、1.198和0.247 d;大红犀猎蝽雌成虫对草地贪夜蛾3龄幼虫的日最大捕食量、瞬时攻击率和处理时间分别为47.619头、0.717和0.021 d;大红犀猎蝽雄成虫对草地贪夜蛾3龄幼虫的日最大捕食量、瞬时攻击率和处理时间分别为43.478头、0.650和0.023 d;大红犀猎蝽的捕食量与害虫密度正相关,搜寻效应与害虫密度负相关。大红犀猎蝽自身密度对捕食作用的干扰效应符合Hassell模型,对草地贪夜蛾3龄幼虫的捕食作用率随自身密度的增加而降低。表明大红犀猎蝽3龄若虫和成虫对草地贪夜蛾3龄幼虫均有一定的捕食能力,大红犀猎蝽成虫的捕食作用大于3龄若虫。