4株鸭源肠球菌的鉴定和致病性

对临床分离的4株鸭源肠球菌郑1株、郑2株、郑3株、北京株和1株粪肠球菌参考菌株进行了系统鉴定,并用SDS-PAGE和Western-blot技术对各菌株细胞壁蛋白图谱进行比较分析。结果5个菌株的形态、染色、生理生化特性均与粪肠球菌特性一致;它们均对青霉素、万古霉素和庆大霉素敏感而对四环素耐药;5个菌株人工感染雏鸭及小白鼠均有致病性,但各菌株间致病力存在差异,北京株最强,参考株最弱,其余3株介于北京株和参考株之间;各菌株的细胞壁蛋白经SDS-PAGE在相对分子质量33 370~131 690之间均显示数十条蛋白带,其中郑2株和北京株在相对分子质量66 840处均有1条染色较深的蛋白带,而用Western-blot分析显示抗北京株胞壁蛋白抗体只能检测到北京株相对分子质量为66 840的抗原蛋白。以上结果表明,这5个被检菌株为致病性粪肠球菌,且致病性以北京株最强。     

铝中毒对蛋鸡白细胞和红细胞免疫功能的影响

采用连续腹腔注射相同体积不同浓度梯度的三氯化铝，建立不同程度的鸡亚慢性铝中毒型，检测铝中毒雏鸡外周血白细胞数(WBC)、淋巴细胞总数、ANAE+T数及红细胞C3b受体花环率和红细胞免疫复合物花环率。结果表明，铝中毒雏鸡外周血白细胞数、淋巴细胞总数、ANAE+T数及红细胞C3b受体花环率均明显低于健康对照组雏鸡，而红细胞免疫复合物花环率明显高于健康对照组雏鸡。上述结果说明, 铝中毒对雏鸡细胞免疫和非特异性免疫功能有明显的抑制作用。     

应用单克隆抗体的免疫组织化学法研究雏鸭体内鸭瘟病毒的分布

本实验应用了免疫组织化学的单克隆抗体间接酶标染色法，对人工感染鸭瘟病毒雏鸭的组织切片进行染色观察。旨在研究病毒在鸭体内分布，对其进行定位。研究结果显示，鸭的心脏、肝脏、脾、胸腺、肠、法氏囊、胰、肺、肾等组织的细胞浆内均出现了染色的特异阳性反应物。结果表明，鸭瘟病毒广泛分布于感染雏鸭的各种组织器官，并造成一定的组织病理变化。     

日粮中添加半胱胺对肉鸡生产性能的影响

选择144只1日龄肉用仔鸡，随机分成4组．每组36只．适应饲养1周后，分别于其基础日粮中添加0、50、100、150mg／kg半胱胺（CS），并于饲喂添加CS日粮后2、4、6、8周屠宰．研究CS对肉仔鸡生长性能的影响。结果表明：（1）在饲料中持续添加CS的前期可影响饲料的适口性。（2）随时间的延长，屠宰率、半净膛率、全净膛率随剂量的加大而升高。（3）在连续添加CS的后期（第6周、第8周）添加50mg／kg的CS组的日增重及料肉比均最好，与对照组相比日增重提高了22．9％、17．8％，料肉比下降了15．7％和3．5％。     

NO、TNF和IL-2与新型鸭肝炎病毒感染雏鸭肝脑组织损伤的关系

用新型鸭肝炎病毒人工感染9日龄健康樱桃谷雏鸭，对感染后12、24、48、96、168h和14d雏鸭血液、肝和脑组织中一氧化氮（NO）含量，血液中肿瘤坏死因子（TNF）和白细胞介素2（IL-2）含量进行了测定，同时对感染雏鸭的组织病理学变化进行了观察。结果表明．血清中NO含量在接种后48h开始升高，一直持续到接种后96h，接种后7d恢复正常；肝脏组织中NO含量仅在接种后24h与对照组比较显著升高，在其他时间未表现有差异；脑组织中NO含量在整个试验期间没有变化。血清中的TNF和IL-2含量在接种后24h均表现升高，接种后96h降低，其他时间无改变。感染雏鸭的肝组织在接种后24h表现出血性坏死性肝炎变化，接种后48～96h呈增生性病变，而接种后各时期脑组织均呈非化脓性脑炎变化。由此表明，新型鸭肝炎病毒感染可导致雏鸭体内NO、TNF和IL-2发生变化，并且与肝组织损伤、疾病的发生发展有关。     

鸡白细胞介素-6活性MTT检测方法的建立

采用白细胞介素-6依赖细胞株B9建立了鸡白细胞介素-6活性的MTT检测方法。每孔培养细胞数在2.5×103~4×104范围内D值与细胞数显示有良好的线性关系,MTT的最佳保留时间为4 h,最低检测限为0.1 U/mL。应用该方法检测了健康艾维茵肉鸡25例,血清chIL-6活性为(4.33±0.75)U/mL,而25例葡萄球菌病患鸡血清chIL-6的活性为(14.05±6.87)U/mL,与健康肉鸡比较差异极显著(P<0.01),为该方法进一步临床应用奠定了基础。     

EAV/DNA指纹图谱条带J与OPAY02型标记对新扬州鸡体质量和生长影响的联合研究

随机选择新扬州鸡大型系和小型系交后代48只，联合研究EAV／DNA指纹图谱带J与OPAY02型2种分子遗传标记与新扬州鸡体质量和生长的关系，结果表明，8、10、26周龄新扬州鸡体质量在2种分子标记的不同组合间比较均存在极显著的差异，尤其是J^-S1^-S2^＋产条带组合遗传标记，8、10、26周龄新扬州鸡体质量均显著或极显著地高于其他JS1S2组合，根据数量性状多基因假说，可以推测J、S1和S2可能是影响鸡增重的多基因，2种分子遗传标记组合J^-S1^-S2^＋遗传标记有望成为鸡体质量选择的遗传标记。     

Architecture of FcαR1 gene in Chinese Han population in Guangdong province

AIM：To construct the SNP haplotypes and the haplotype blocks of FcαR1 gene in Chinese Han population in Guangdong province.METHODS：Genomic DNA was extracted from 100 healthy Chinese Han subjects.Genotypes of the SNPs in FcαR1 gene were determined by PCR and direct sequencing.Constructing the SNP haplotypes and the haplotype blocks of FcαR1 gene by 12 SNPs that is its minor allele frequency were more than 0.1 with Haploview soft.RESULTS：There are 3 haplotype blocks in FcαR1 gene in Chinese Han population in Guangdong province.The number of haplotypes in each haplotype blocks was from 3 to 7 (average 5.33).However,the number of common haplotypes in each blocks was from 2 to 3 (average 2.3).CONCLUSION：Our results show the existence of the block-like structures and the common haplotypes in each block in FcαR1 gene.It provides a useful tool for the genome research and the mapping of disease contributing genes/variants in this gene.     

Effect of endogenous H2S on pulmonary hypertension during acute lung injury induced by LPS and its interaction with NO

AIM: To investigate the effect of H2S on pulmonary artery hypertension during acute lung injury induced by LPS and the interaction between the systems of hydrogen sulfide (H2S)/cystathionine-β-lyase (CSE) and nitric oxide (NO)/nitric oxide synthase (NOS) in this process. METHODS: Seventy-two adult male rats were randomly divided into four groups: control group, LPS group, LPS+L-NAME group and LPS+propargylglycine (PPG) group. Mean pulmonary artery pressure (mPAP) of each rat was examined at 2 h, 4 h, 6 h and 8 h after treatment. H2S and NO contents in plasma, NO content, iNOS, cNOS and CSE activity in lung were measured at 4 h or 8 h after treatment, respectively. Expression of iNOS in lung tissue was also detected by immunohistochemistry technique, and the injury of lung was evaluated with morphological changes under microscope. RESULTS: LPS could induce severe lung injury, and mPAP, NO content, iNOS activity and its protein expression in LPS group significantly increased, but cNOS activity, H2S content and CSE activity decreased compared with those of control group. Administration of L-NAME before LPS could attenuate the changes induced by LPS. Pre-administration of PPG, a CSE inhibitor, exacerbated the injury by LPS, but there was no prominent variation in cNOS activity. CONCLUSION: Reduced endogenous H2S could increase pulmonary artery hypertension during acute lung injury induced by LPS. There is a negative effect between H2S/CSE system and NO/NOS system in this process.     

Effects of activated state of T cells from human peripheral blood on absorption of photosensitizer hematoporphyrin monomerthyl ether

AIM: To investigate the characterization of absorption of hematoporphyrin monomerthyl ether (HMME), a domestic new generation photosensitizer product, by activated T cells from human peripheral blood. METHODS: Evaluation was performed by flow cytometry on the effects of incubating concentration and time of HMME on absorption by activated T cells. Lymphocytes were separated from human peripheral blood by density gradient centrifugation with Ficoll and T cells were activated with polyclonal stimulators PHA and PDB+Ion. To analyze the effects of HMME incubating doses on the absorption of activated T cells, the cultural lymphocytes were incubated with a serial doses of HMME for 1 h and HMME absorption were measured by FACS after immuno-staining with anti-CD3 antibody. To test the impact of HMME incubating time on the absorption of activated T cells, the cultural lymphocytes were incubated with HMME for various times and HMME absorption were measured by FACS after immuno-staining with anti-CD3 antibody. RESULTS: The HMME absorption-dose curve and absorption-time curve were shifted to right and up in the activated T cells as compared to resting T cells. HMME absorptions of activated T cells were statistic significantly larger than that of resting T cells in the doses between 5 mg/L to 20 mg/L. HMME absorptions of either activated T cells or resting T cells underwent a gradual increase with the incubation-time in HMME at concentration of 10 mg/L. HMME absorptions of activated T cells were statistic significantly larger than that of resting T cells in the incubation-time between 15 to 60 min. CONCLUSION: The differences of HMME absorption between activated T cells and resting T cells depend on the incubation times and doses of HMME. HMME absorption of activated T cells are significantly larger than that of resting T cells in certain incubation-times and doses. These results suggest that incubation time and dose associated with HMME-PDT therapeutic windows will be created for selective deletion of activated T cells.