Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

Informing Community Engagement in Sea Turtle Conservation by Examining Non-Conservation-Related Participation in Northeast Brazil

Participation in conservation programs is often viewed as a necessity, but understanding of this participation is limited. This research takes a different approach by also examining participation in non-conservation-related activities. Questionnaires (N = 339) were administered in four Brazilian coastal communities where the national sea turtle conservation project conducts monitoring and outreach. Employing social capital as a conceptual framework, measurements included participation in civic, community, and social activities along with group participation and collective action. Confirmatory factor analysis was used for validating measures of social capital and conservation perceptions. Structural equation modeling indicated that social capital has a positive relationship with participation in sea turtle conservation, suggesting that general levels of participation and community norms of participation could be just as influential as conservation perceptions to engage communities in conservation. Information gained by deconstructing participation may be used to design and target conservation programs, improving overall participation.     

贮藏温度对采后“金艳”猕猴桃品质和后熟的影响

以"金艳"猕猴桃为试材,采用不同温度贮藏的方法,研究了温度对果实品质和呼吸强度的影响。结果表明:1℃贮藏可以很好的保持果实品质,呼吸强度峰值出现晚,保持了较高的果肉硬度;5℃和10℃贮藏能够促进果实的后熟,加速呼吸强度峰值的到来、果实硬度的下降和可溶性固形物含量的上升;但35℃贮藏的果实7d后会失去商品价值,发生腐败;16℃贮藏果实后熟品质不佳。在整个贮藏过程中,果实干物质含量在上述温度条件下贮藏变化都不明显,但在一定范围内,干物质含量会随着温度的上升而有所增加。     

缺素胁迫对软枣猕猴桃幼苗生长的影响

以软枣猕猴桃幼苗为试材,研究了缺铁(Fe)、镁(Mg)、磷(P)、钙(Ca)、钾(K)、氮(N)6种元素对其幼苗生长及生理指标的影响。结果表明:不同缺素处理对其幼苗的生长发育及生理指标均能产生不同程度的影响,其缺钙(-Ca)、钾(-K)、氮(-N)对软枣猕猴桃幼苗根长、株质量、株高影响显著;游离脯氨酸(Pro)含量、丙二醛(MDA)含量、过氧化物酶(POD)活性及超氧化物歧化酶(SOD)活性均明显增加。     

三种地被菊种子萌发及幼苗生长的化感效应比较

以天人菊、金鸡菊和蛇鞭菊为试材,比较两两混种与单种在种子萌发和幼苗生长阶段相互间的化感效应强度。结果表明:两两混种下,金鸡菊、蛇鞭菊对天人菊生长起促进作用,化感效应强度为金鸡菊蛇鞭菊;天人菊、蛇鞭菊对金鸡菊生长起抑制作用,化感效应强度为天人菊蛇鞭菊;金鸡菊、天人菊对蛇鞭菊生长起抑制作用,化感效应强度为金鸡菊天人菊。     

甜瓜黄斑病毒SYBR Green Ⅰ实时荧光定量PCR方法

为建立检测甜瓜黄斑病毒(melon yellow spot virus, MYSV)的SYBR Green Ⅰ实时荧光定量PCR(qPCR)方法。基于MYSV核衣壳蛋白基因保守序列设计qPCR特异性引物对,针对引物退火温度、引物浓度、特异性和敏感性进行系列优化。结果显示,优化后的qPCR方法最适退火温度为61.3℃,最适引物浓度为0.65μmol·L^-1,特异性强,灵敏度高,比PCR高100倍。以携带目的基因片段的重组质粒为标准品,构建的qPCR标准曲线循环阈值与模板浓度呈良好的线性关系,相关系数为0.999 7。实验样品验证表明建立的qPCR方法可用于MYSV的定量检测。     

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析

为更好地利用老芒麦(Elymus sibiricus L.)野生资源,本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1 723份野生披碱草属种质资源,通过表型特征观测和流式细胞仪检测,共鉴定出了990份野生老芒麦种质,246份垂穗披碱草种质,并测得老芒麦的DNA含量在5.86～7.30 Gb之间,平均DNA含量为6.66 Gb;垂穗披碱草材料的DNA含量在9.50～10.36 Gb之间,平均DNA含量为9.97 Gb。研究结果显示,在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛,分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01),与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系,可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据,为老芒麦种质资源挖掘和育种应用提供了材料基础。     

植物基因启动子的克隆方法及其应用

植物基因的表达调控已成为分子生物学研究热点,启动子是基因表达调控的重要顺式元件,启动子的克隆对于研究基因表达调控、构建基因工程载体、表达目的蛋白有着重要的意义。启动子克隆的方法很多,从常用的启动子陷阱技术筛选启动子到PCR方法的应用,此后相继问世的一些基于PCR的克隆启动子技术,如载体锚定PCR、反向PCR、接头PCR、交错热不对称PCR等,为克隆启动子提供了更可靠,更合理的方法。本文着重介绍了几种植物基因启动子的克隆方法,分析了它们的优缺点,并展望了今后的研究前景。