观赏植物养护专用缓释肥在3种植物上的应用研究初报

就一种新型观赏植物养护专用缓释肥在醉蝶花、罗汉松、莞香3种观赏植物上的应用效果进行初步探讨。结果表明:缓释肥可促进3种观赏植物的生长,与复合肥相比,该缓释肥具有肥效慢、肥效持续时间长、肥料利用效率高的特点。试验还发现,适量施用缓释肥可促进醉蝶花开花和延长开花期,且该缓释肥覆土施用要明显优于表土施用。     

森林灭火机具组合使用的研究

介绍了扑灭低、中、高强度林火时应使用的灭火机具,并给出了各种机具的合理组合建议。     

CD147 monoclonal antibody-mediated nanoparticles for gene therapy to target lung cancer cells

AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells.     

长白山区特色野菜合苞橐吾栽培技术

总结介绍了长白山特色野菜资源合苞橐吾品种特点、观赏性栽培种苗繁育技术和病虫害防治等关键栽培技术。合苞橐吾应用前景广泛、生产经济效益较大,规模化育苗、栽培与管理,可满足人们对优质合苞橐吾种苗的需求,实现合苞橐吾长白山特色山野菜的开发价值。     

兰州大尾羊解偶联蛋白3(UCP3)基因荧光定量PCR检测方法的研究

试验通过提取兰州大尾羊不同组织中总RNA,反转录获得总cDNA样品,建立了用SYBR荧光定量PCR法检测兰州大尾羊解偶联蛋白(UCP3)基因mRNA在不同组织中的相对表达量的检测方法,并进行批内、批间重复性检验。结果表明:UCP3和GADPH基因标准曲线回归方程分别为CtUCP3=-3.2343x+41.6198和CtGADPH=-3.2851x+38.0344,相关系数(r2)分别为0.9958和0.9986,扩增效率分别为103%和97%;批内、批间重复性测定的变异系数分别为小于1.4%和10%。     

头孢类抗生素对中黑防杨叶片分化的影响

探讨了头孢唑啉钠、头孢曲松钠、头孢霉素3种头孢类抗生素对农杆菌EHA105的抑制作用,以及对中黑防杨叶片生长和分化的影响。结果表明:头孢霉素和头孢曲松钠的抑菌效果优于头孢唑啉钠,但二者对叶片分化的抑制性太强,而750 mg/L的头孢唑啉钠对叶片分化影响很弱,仅比对照低3%,同时抑菌效果好。因此确定了由农杆菌介导的中黑防2号杨遗传转化时的抗生素为头孢唑啉钠其使用浓度为750 mg/L。     

探究读者心理提高服务质量——以海南大学图书馆为例

高校图书馆的服务对象是文化水平较高的读者,研究和掌握读者心理,了解读者的需求,有助于问题和矛盾的解决,使得图书馆的教育职能和情报职能充分发挥,更好地为高校读者服务。     

田面无水层条件下渠灌稻田土壤水分影响因素及预测模型研究

通过田间试验,研究了在田面无水层条件下拔节孕穗期、抽穗开花期及乳熟期等3个主要生育阶段渠灌稻田土壤水分的影响因子及预测模型.相关分析及多元逐步回归分析表明,田间小气候因子中,气温、空气相对湿度及风速对渠灌稻田土壤水分影响较大.误差分析结果也表明,对气温、相对湿度及风速等逐步回归建立的多元线性模型,最大测量误差分别为5.38%、3.35%和3.84%,拟合效果满意.研究成果为田面无水层条件下渠灌稻田土壤水分的预报奠定了基础.     

牛羊屠宰粪便优势发酵菌株的分离、培养及筛选

应用选择性培养基在牛羊屠宰粪便中进行纤维分解菌、固氮菌、解磷菌和解钾菌的分离、培养，分离得到201株菌株。对其中的18株纤维分解菌进行纤维素酶酶活力的测定，经测定1，6，7，13，15，18号菌株酶活力相对较高，每mL培养物中的酶活力均高于500U／g，为将来进一步进行发酵菌剂的研究提供了优势候选菌株。     

添加茭白鞘叶栽培双孢蘑菇初探

检测表明茭白鞘叶中含有丰富的有机质与氮源,分别是稻草的2倍和4倍,是栽培双孢蘑菇的理想替代原料。在传统稻草配方中添加茭白鞘叶栽培蘑菇的试验,研究结果表明:走菌速度随配方中茭白鞘叶含量的增加而减慢,茭白鞘叶配方的蘑菇现蕾期和开始采菇时间分别比稻草配方迟3、4d;稻草与茭白鞘叶各占50%配方为最佳,单季产量达到20.45kg·m-2,几乎和传统配方持平,而投入产出比可以达到4.479,超过传统配方的3.439,并且菇体单粒重和传统配方没有显著差异,而且鲜菇的品质经分析和传统配方几乎无异,具有良好的经济效益。本研究初步明确了茭白鞘叶栽培蘑菇的工艺流程、栽培技术与克服出现问题的方法,而且利用茭白鞘叶栽培蘑菇既缓解了蘑菇主产区稻草紧缺的局面,又是解决茭白鞘叶资源浪费和环境污染的可行途径。