全文获取类型
收费全文 | 1363篇 |
免费 | 66篇 |
国内免费 | 6篇 |
专业分类
林业 | 72篇 |
农学 | 77篇 |
基础科学 | 2篇 |
180篇 | |
综合类 | 65篇 |
农作物 | 40篇 |
水产渔业 | 104篇 |
畜牧兽医 | 783篇 |
园艺 | 13篇 |
植物保护 | 99篇 |
出版年
2022年 | 5篇 |
2021年 | 26篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 35篇 |
2017年 | 40篇 |
2016年 | 40篇 |
2015年 | 45篇 |
2014年 | 48篇 |
2013年 | 99篇 |
2012年 | 79篇 |
2011年 | 85篇 |
2010年 | 33篇 |
2009年 | 44篇 |
2008年 | 61篇 |
2007年 | 69篇 |
2006年 | 77篇 |
2005年 | 80篇 |
2004年 | 69篇 |
2003年 | 57篇 |
2002年 | 38篇 |
2001年 | 42篇 |
2000年 | 49篇 |
1999年 | 42篇 |
1998年 | 6篇 |
1997年 | 11篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 14篇 |
1991年 | 15篇 |
1990年 | 16篇 |
1989年 | 15篇 |
1988年 | 16篇 |
1987年 | 18篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1979年 | 9篇 |
1977年 | 7篇 |
1974年 | 5篇 |
1972年 | 7篇 |
1970年 | 3篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1965年 | 3篇 |
排序方式: 共有1435条查询结果,搜索用时 421 毫秒
1.
F Urayama T Sato H Shibuya W Shirai M Matsutani R Yamazaki 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1249-1252
An apocrine adenocarcinoma was observed in the subcutis of the abdomen of golden hamster. Histologically, the tumor cells irregularly formed multiple layers of cysts and some detached cells were presented in the cystic space. PAS stain with alpha-amylase digestion revealed PAS-positive alpha-amylase-resistant granules in the cytoplasm. Immunohistochemically, cytokeratin was demonstrated in the tumor cells. By electron microscopy, the tumor cells had an oval nucleus with invagination, abundant cytoplasmic organelles and microvilli protruding into the intercellular spaces. 相似文献
2.
Fluid accumulation in mouse ligated intestine inoculated with the vascular permeability factor produced by Bacillus cereus. 总被引:1,自引:0,他引:1
K Shinagawa K Sato H Konuma N Matsusaka S Sugii 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(2):167-171
Partially purified vascular permeability (VP) factor (VPF) of Bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (MIL) and rabbit (RIL), suggesting that the VP activity may correlate with fluid accumulation in ligated intestinal loops of these animals. Fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (IDU) of partially purified VPF, whereas it was found at 2 hr in all loops with 400-600 IDU of partially purified VPF. In rabbit intestinal loops with 120-190 IDU of partially purified VPF, fluid accumulation was observed at 6 hr. From these findings, VPF produced by B. cereus can be easily detected in both MIL and RIL. The intestinal tissue of mouse intestinal loops was histopathologically damaged at different concentrations of the VPF to induce fluid accumulation. With 50 IDU of partially purified VPF, severe edema was found in the laminia proprial layer and submucosa. With 600 IDU of partially purified VPF, on the other hand, severe necrosis in the surface epithelium of villus and laminia proprial layer, and hyperemia in the submucosa were observed, suggesting that partially purified VPF may be cytotoxic and/or intestinecrotic. 相似文献
3.
4.
5.
Keisuke SASAKI Tomotaro YOSHIDA Kengo KOGA Takashi HARAGUCHI Kazunori OHASHI Yosuke AOYAGI 《Animal Science Journal》2004,75(6):557-562
The effect of insulin on the ascorbate recycling system in the chicken liver was examined. First, insulin was injected subcutaneously into the chicken, and liver glutathione‐dependent dehydroascorbate reductase (GSH‐DHAR) activity was determined. Insulin increased liver GSH‐DHAR activity, but did not affect plasma and liver ascorbate concentration. Dehydroascorbate increased plasma and liver ascorbate levels, and liver GSH‐DHAR activity. However, distinct changes in plasma insulin level were not observed by dehydroascorbate injection. In addition, reduction of external dehydroascorbate in cultured chicken hepatocytes could not be observed in an insulin‐deprived culture, although the cells reduced external dehydroascorbate in a serum‐free culture with insulin. We concluded that insulin affects the ascorbate recycling system as an essential factor in the chicken liver. 相似文献
6.
Familial Congenital Methemoglobinemia in Pomeranian Dogs Caused by a Missense Variant in the NADH‐Cytochrome B5 Reductase Gene 下载免费PDF全文
H. Shino Y. Otsuka‐Yamasaki T. Sato K. Ooi O. Inanami R. Sato M. Yamasaki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):165-171
Background
In veterinary medicine, congenital methemoglobinemia associated with nicotinamide adenine dinucleotide (NADH)‐cytochrome b5 reductase (b5R) deficiency is rare. It has been reported in several breeds of dogs, but little information is available about its etiology.Objectives
To analyze the NADH‐cytochrome b5 reductase gene, CYB5R3, in a Pomeranian dog family with methemoglobinemia suspected to be caused by congenital b5R deficiency.Animals
Three Pomeranian dogs from a family with methemoglobinemia were analyzed. Five healthy beagles and 5 nonrelated Pomeranian dogs without methemoglobinemia were used as controls.Methods
Methemoglobin concentration, b5R activity, and reduced glutathione (GSH) concentration were measured, and a turbidity index was used to evaluate Heinz body formation. The CYB5R3 genes of the affected dog and healthy dogs were analyzed by direct sequencing.Results
Methemoglobin concentrations in erythrocytes of the affected dogs were remarkably higher than those of the control dogs. The b5R activity of the affected dogs was notably lower than that of the control dogs. DNA sequencing indicated that this Pomeranian family carried a CYB5R3 gene missense variant (ATC→CTC at codon 194) that resulted in the replacement of isoleucine (Ile) by leucine (Leu).Conclusions and Clinical Importance
This dog family had familial congenital methemoglobinemia caused by b5R deficiency, which resulted from a nonsynonymous variant in the CYB5R3 gene. This variation (c.580A>C) led to an amino acid substitution (p.Ile194Leu), and Ile194 was located in the proximal region of the NADH‐binding motif. Our data suggested that this variant in the canine CYB5R3 gene would affect function of the b5R in erythrocytes. 相似文献7.
8.
Yosuke SASAKI Satoshi SEKIGUCHI Ryoko UEMURA Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):333-335
This study compared the reproductive and growth performances of pigs before and after depopulation and
restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after
depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and
restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and
respiratory syndrome and Aujeszky’s disease, remained undetected on the farms. Compared with before
depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born
was higher, the postweaning mortality rate was lower, and the age at slaughter was lower
(P<0.05). In summary, depopulation and restocking improved the reproductive and growth
performances of pigs. 相似文献
9.
Oriental persimmon (Diospyros kaki) originated in Eastern Asia, and many indigenous cultivars have been developed in China, Japan, and Korea. These cultivars are classified into four groups based on their natural astringency loss on the tree and seed formation: pollination-constant non-astringent (PCNA), pollination-variant non-astringent (PVNA), pollination-constant astringent (PCA), and pollination-variant astringent (PVA). PCNA is the most desirable type because the fruit can be eaten without any postharvest treatment; therefore, one of the goals of our persimmon breeding programs is to release superior PCNA cultivars. The PCNA genotype is recessive to the other three non-PCNA genotypes, and PCNA-type F1 offspring are obtained exclusively from crosses among PCNA genotypes. Moreover, the number of superior PCNA cross-parents have been limited. In the late 1980s, inbreeding depression became obvious, especially in terms of fruit size, tree vigor, and productivity. To mitigate the inbreeding, a backcross program using PCNA [(non-PCNA × PCNA) × PCNA] was started in 1990. This process, however, was inefficient because only 15% of the offspring were PCNA, and all offspring had to be grown to the fruiting stage. Therefore, molecular markers linked to the PCNA locus were developed for discriminating PCNA offspring. A molecular marker linked to Chinese PCNA has also been developed. 相似文献
10.
Chie SUZUKI Yosuke SAKAGUCHI Hiroyoshi HOSHI Koji YOSHIOKA 《The Journal of reproduction and development》2016,62(1):79-86
The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced
in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium
(PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates
of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured
with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of
LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with
other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and
Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured
with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes
involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3,
CPT1, CPT2 and KAT) in Day-7 blastocysts were
significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching
ability and quality of porcine blastocysts produced in vitro, as determined by ATP content,
blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of
LR-BSA arise from lipids bound to albumin. 相似文献